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公开(公告)号：US20210246483A1
公开(公告)日：2021-08-12
申请号：US17240093
申请日：2021-04-26
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Tino Alavie , Stephen Wesley Leonard
Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.
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公开(公告)号：US10988794B2
公开(公告)日：2021-04-27
申请号：US16280842
申请日：2019-02-20
Applicant: Qvella Corporation
Inventor： Samad Talebpour , Aye Aye Khine , Tino Alavie , Stephen Wesley Leonard
Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.
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13.发明授权公开(公告)号：US09063136B2
公开(公告)日：2015-06-23
申请号：US13388654
申请日：2010-07-30
Applicant: Samad Talebpour , Aye Aye Khine , Stephen W. Leonard , Robert Maaskant , Tino Alavie
Inventor： Samad Talebpour , Aye Aye Khine , Stephen W. Leonard , Robert Maaskant , Tino Alavie
IPC: G01N33/543 , B01L3/00 , C12M3/06 , C12M1/12 , C12M1/00 , C12M1/42 , C12M1/34 , C12N1/06 , C12N11/14 , C12N13/00 , G01N33/50
CPC classification number: G01N33/5438 , B01L3/502715 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0645 , B01L2300/0809 , C12M23/16 , C12M25/00 , C12M25/04 , C12M29/10 , C12M35/02 , C12M41/38 , C12N1/066 , C12N11/14 , C12N13/00 , G01N33/5005
Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.
Abstract translation: 本发明提供一种微流体装置及其用于浓缩和捕获细胞的方法。 在层流下相对于样品施加脉冲非法拉第电场，这导致带电分析物的浓度和捕获。 有利地，选择脉冲定时以避免与通道内的离子屏蔽相关的问题。 通道内的至少一个电极涂有绝缘层，以防止法拉第电流在通道中流动。 在脉冲施加单极电压给电极的情况下，样品内的带电分析物经由瞬时电泳力朝向其中一个电极移动。
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公开(公告)号：US11788114B2
公开(公告)日：2023-10-17
申请号：US16510253
申请日：2019-07-12
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Robert Maaskant , Tino Alavie
IPC: C12M1/42 , C12M1/00 , C12N1/06 , C12N1/08 , C12N13/00 , C12Q1/6806 , C12Q3/00 , C12Q1/686 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/6806 , B01L3/502715 , B01L3/502738 , B01L3/502753 , B01L7/52 , C12M35/02 , C12M47/06 , C12N1/066 , C12N1/08 , C12N13/00 , C12Q1/686 , C12Q3/00 , B01L2200/0631 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/087 , B01L2300/0809 , B01L2300/0858 , B01L2300/0874 , B01L2300/0887 , B01L2300/14 , B01L2300/1833 , B01L2400/0694
Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
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公开(公告)号：US11702647B2
公开(公告)日：2023-07-18
申请号：US16881479
申请日：2020-05-22
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Robert Maaskant , Tino Alavie
IPC: C12N15/10 , B01L3/00 , C12Q1/6806 , C12Q1/24 , C08F220/28
CPC classification number: C12N15/1003 , B01L3/5021 , B01L3/5027 , C08F220/286 , C12Q1/24 , C12Q1/6806 , C08G2261/1452
Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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Patent Agency Ranking
公开(公告)号：US10662420B2
公开(公告)日：2020-05-26
申请号：US15612091
申请日：2017-06-02
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Robert Maaskant , Tino Alavie
Abstract: Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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公开(公告)号：US10233483B2
公开(公告)日：2019-03-19
申请号：US14901200
申请日：2014-07-03
Applicant: Qvella Corporation
Inventor： Samad Talebpour , Aye Aye Khine , Tino Alavie , Stephen Wesley Leonard
Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.
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公开(公告)号：US09707555B2
公开(公告)日：2017-07-18
申请号：US14648161
申请日：2013-11-26
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Robert Maaskant , Tino Alavie
CPC classification number: B01L3/5021 , B01L3/50215 , B01L3/5027 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/0816 , B01L2300/0864 , B01L2300/1833 , C12Q1/6806 , C12Q2523/307 , C12Q2527/125 , C12Q2527/137
Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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19.发明授权公开(公告)号：US12253513B2
公开(公告)日：2025-03-18
申请号：US17057088
申请日：2019-05-24
Applicant: QVELLA CORPORATION
Inventor： Samad Talebpour , Aye Aye Khine , Vilcy Parmar , Sukhdev Manku , Alaleh Samiei
Abstract: Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.
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公开(公告)号：US20230416722A1
公开(公告)日：2023-12-28
申请号：US18208713
申请日：2023-06-12
Applicant: QVELLA CORPORATION
Inventor： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
IPC: C12N15/10 , B01L3/00 , C12Q1/6806 , C12Q1/24 , C08F220/28
CPC classification number: C12N15/1003 , B01L3/5021 , B01L3/5027 , C12Q1/6806 , C12Q1/24 , C08F220/286 , C08G2261/1452
Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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