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    Protein-protein interactions in neurodegenerative diseases
    11.
    发明申请
    Protein-protein interactions in neurodegenerative diseases 审中-公开
    蛋白质 - 蛋白质在神经退行性疾病中的相互作用

    公开(公告)号：US20020115119A1

    公开(公告)日：2002-08-22

    申请号：US09973063

    申请日：2001-10-10

    Applicant: Myriad Genetics, Inc.

    Inventor： Jean-Mark Roch ,  Paul L. Bartel ,  Karen Heichman

    IPC: G01N033/567

    CPC classification number: G01N33/6896 ,  A61K38/1709 ,  C07K14/47 ,  C07K14/4711 ,  C07K16/18 ,  C07K16/2833 ,  C07K2317/32 ,  C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/156 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/28 ,  C12Q2565/201

    Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.

    Abstract translation: 本发明涉及发现包括阿尔茨海默氏病（AD）在内的神经变性疾病的发病机理中的蛋白质 - 蛋白质相互作用。 因此，本发明涉及这些蛋白质和/或其片段的复合物，复合物的抗体，神经变性疾病的诊断（包括诊断病因的倾向和诊断），药物筛选调节剂 本文所述的蛋白质的相互作用以及在本文所述的蛋白质共有的途径中鉴定另外的蛋白质。

    Method for screening anticancer agent
    12.
    发明授权
    Method for screening anticancer agent 失效
    抗癌剂筛选方法

    公开(公告)号：US07407745B1

    公开(公告)日：2008-08-05

    申请号：US09937162

    申请日：2000-03-23

    Applicant: Yoshihiro Sowa ,  Tetsuro Orita

    Inventor： Yoshihiro Sowa ,  Tetsuro Orita

    IPC: C12Q1/68

    CPC classification number: C12Q1/6897 ,  C12Q2565/201

    Abstract: By analyzing the mechanism for inducing a histone deacetylase inhibitor (Trichostatin A)-mediated expression of cyclin-CDK inhibitory factor having a proliferation suppressing effect (tumor-suppressing effect), it was revealed that the binding of Sp3 to the Sp1 binding sequence within a promoter is important in the expression of the above factor. Thus, a novel antitumor agent can be developed by screening a pharmaceutical agent capable of elevating Sp3 activity.

    Abstract translation: 通过分析诱导组蛋白脱乙酰酶抑制剂（曲古抑菌素A）介导的具有增殖抑制作用（肿瘤抑制作用）的细胞周期蛋白CDK抑制因子的表达机制，揭示了Sp3与Sp1结合序列的结合 启动子在上述因子的表达中是重要的。 因此，可以通过筛选能够提高Sp3活性的药剂来开发新的抗肿瘤剂。

    Genetic Screen for Interaction Interface Mapping
    13.
    发明申请
    Genetic Screen for Interaction Interface Mapping 审中-公开
    交互界面映射的遗传屏幕

    公开(公告)号：US20080044815A1

    公开(公告)日：2008-02-21

    申请号：US10558863

    申请日：2004-05-31

    Applicant: Paul Michael Watt ,  Richard Hopkins ,  Marie Bogoyevitch

    Inventor： Paul Michael Watt ,  Richard Hopkins ,  Marie Bogoyevitch

    IPC: C12Q1/68

    CPC classification number: C12N15/1055 ,  C12Q1/6897 ,  C12Q2565/201

    Abstract: The present invention provides improved reverse hybrid assay methods for identifying amino acid residues within a protein that are required for its interaction or physical association with another protein, wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single step, thereby reducing the incidence of uninformative mutations in the protein of interest that are detected.

    Abstract translation: 本发明提供了改进的用于鉴定蛋白质内与氨基酸残基相互作用或与另一种蛋白质相互作用所必需的氨基酸残基的改进的反向杂交测定方法，其中分析目标蛋白质与其结合配偶体蛋白质之间相互作用的破坏， 的所述目的蛋白质的突变，以及在一个步骤中同时测定目的蛋白质和另一个结合配偶体之间的相互作用的维持，从而降低检测到的感兴趣的蛋白质中的不知情突变的发生率。

    Two hybrid assay that detects HIV-1 reverse transcriptase dimerization
    17.
    发明授权
    Two hybrid assay that detects HIV-1 reverse transcriptase dimerization 失效
    检测HIV-1逆转录酶二聚化的两种杂交测定法

    公开(公告)号：US06812025B2

    公开(公告)日：2004-11-02

    申请号：US10009433

    申请日：2002-06-12

    Applicant: Stephen P. Goff ,  Gilda Tachedjian

    Inventor： Stephen P. Goff ,  Gilda Tachedjian

    IPC: C12N516

    CPC classification number: C12Y207/07049 ,  C07K2319/00 ,  C12N9/1276 ,  C12Q1/6897 ,  C12Q2565/201

    Abstract: This invention provides methods of determining whether a compound inhibits HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides a method of determining whether a compound enhances formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound enhances formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase.

    Abstract translation: 本发明提供了确定化合物是否抑制HIV-1逆转录酶的方法。 本发明提供了确定化合物是否抑制HIV-1逆转录酶的p66和p51亚基多肽之间复合物形成的方法。 本发明提供了确定化合物是否增强HIV-1逆转录酶的p66和p51亚基多肽之间的复合物形成的方法。 本发明提供了确定化合物是否抑制HIV-1逆转录酶的两个p66亚基多肽之间复合物形成的方法。 本发明提供了确定化合物是否增强HIV-1逆转录酶的两个p66亚基多肽之间复合物形成的方法。

    Two-hybrid double screening system and method
    18.
    发明申请
    Two-hybrid double screening system and method 审中-公开
    双杂交双筛选系统及方法

    公开(公告)号：US20030211523A1

    公开(公告)日：2003-11-13

    申请号：US10354931

    申请日：2003-01-30

    Inventor： Shuang Zhang ,  Edward B. Ziff ,  Jun Zhong

    IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  C12N001/18

    CPC classification number: C40B30/04 ,  C12Q1/6897 ,  C12Q2565/201

    Abstract: A method of identifying proteins able to interact via protein-protein interactions with a protein of interest, comprising (a) performing a first assay to identify cells expressing a reporter gene, wherein the first assay is transcription-based two-hybrid screening system; (b) obtaining DNA from the cells identified in step (a) via a PCR procedure; and (c) performing a second assay on the DNA obtained in step (b), wherein the second assay is a non-transcription-based two-hybrid screening system, to identify cells expressing a reporter gene, wherein positive cells identified in step (a) that are found to be positive in step (c) express a protein able to interact via protein-protein interactions with a protein of interest.

    Abstract translation: 一种鉴定能够通过蛋白质 - 蛋白质相互作用与感兴趣的蛋白质相互作用的蛋白质的方法，其包括（a）进行第一测定以鉴定表达报告基因的细胞，其中所述第一测定是基于转录的双杂交筛选系统; （b）通过PCR方法从步骤（a）中鉴定的细胞获得DNA; 和（c）对步骤（b）中获得的DNA进行第二次测定，其中第二次测定是基于非转录的双杂交筛选系统，以鉴定表达报道基因的细胞，其中在步骤 a）在步骤（c）中发现是阳性的，表达能够通过蛋白质 - 蛋白质与感兴趣的蛋白质相互作用相互作用的蛋白质。

    Reverse n-hybrid screening method
    19.
    发明申请
    Reverse n-hybrid screening method 审中-公开
    反向杂交筛选法

    公开(公告)号：US20030211495A1

    公开(公告)日：2003-11-13

    申请号：US10221276

    申请日：2003-01-14

    Inventor： Richard Hopkins ,  Ilya Serebriiskii ,  Paul Michael Watt ,  Erica Golemis

    IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  C12N015/85

    CPC classification number: G01N33/5011 ,  C12Q1/025 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/502 ,  C12Q2565/207 ,  C12Q2565/201

    Abstract: The present invention relates provides an improved reverse n-hybrid screening method for identifying antagonists or inhibitors of biological interactions, wherein multiple reporter genes are employed to distinguish antagonists or inhibitors of one interaction from those antagonists or inhibitors of other interactions, particularly where each of said interactions involves one or more common interacting partners. A further aspect of the invention provides novel expression vectors for performing the inventive method, particularly in high throughput screens.

    Abstract translation: 本发明涉及提供用于鉴定生物相互作用的拮抗剂或抑制剂的改进的反向杂交筛选方法，其中使用多个报告基因来区分一些相互作用的拮抗剂或抑制剂与那些拮抗剂或其它相互作用的抑制剂，特别是其中每个所述 交互涉及一个或多个常见的互动伙伴。 本发明的另一方面提供用于执行本发明方法的新型表达载体，特别是在高通量筛选中。

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