公开(公告)号：US11499143B2

公开(公告)日：2022-11-15

申请号：US15521104

申请日：2015-10-23

Applicant: Kikkoman Corporation

Inventor： Yosuke Masakari ,  Airi Komatsuzaki ,  Atsushi Ichiyanagi

IPC: C12N9/06 ,  C12Q1/26 ,  C12Q1/00 ,  G01N27/416 ,  C12M1/40 ,  G01N27/327

Abstract: Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.

公开(公告)号：US11447766B2

公开(公告)日：2022-09-20

申请号：US16064644

申请日：2016-12-21

Applicant: Daesang Corporation

Inventor： Baek Joong Kim ,  Tae Gun Kim ,  Min Su Kim ,  Tae Yong Kim ,  Eun Bum Song

IPC: C12M1/00 ,  C12N11/00 ,  C12N11/10 ,  C12N9/90 ,  C12M1/12 ,  C12M1/26 ,  C12M1/02 ,  C12M1/04 ,  C12N15/77 ,  C12M1/40

Abstract: An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.

公开(公告)号：US20220195490A1

公开(公告)日：2022-06-23

申请号：US17578802

申请日：2022-01-19

Applicant: Q-linea AB

Inventor： Markus Klintstedt ,  Harer Osman ,  Mats Gullberg

IPC: C12Q1/24 ,  C12M1/40 ,  C12M1/26 ,  G01N1/40

Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.

公开(公告)号：US20220105475A1

公开(公告)日：2022-04-07

申请号：US17551826

申请日：2021-12-15

Applicant: UNM RAINFOREST INNOVATIONS ,  NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC

Inventor： Susan Lynne REMPE ,  Ying-Bing JIANG ,  Juan VANEGAS ,  C. Jeffrey BRINKER ,  Joseph L. CECCHI

IPC: B01D69/14 ,  B01D53/22 ,  B01D53/62 ,  B01D67/00 ,  B01D69/10 ,  C12M1/40 ,  C12M1/12

Abstract: A membrane structure for moving a gaseous object species from a first region having an object species first concentration, through the membrane structure, to a second region having an object species second concentration different from the first concentration is described. The membrane includes a supporting substrate having a plurality of pores therethrough, each of the plurality of pores defined by a first end, a second end and a surface of the supporting substrate extending between the first end and the second end as well as a nanoporous layer within the plurality of pores, wherein the nanoporous layer comprises a hydrophilic layer and a hydrophobic layer. The membrane also includes a liquid transport medium within the hydrophilic layer. The liquid transport medium includes a liquideous permeation medium and at least one enzyme within the liquideous permeation medium. The at least one enzyme is reinforced by at least one stabilizing component.

公开(公告)号：US11077413B2

公开(公告)日：2021-08-03

申请号：US16083733

申请日：2017-03-17

Applicant: ALKYMAR AS

Inventor： Olav Asle Djupevåg ,  Arnstein Haugen ,  Ole Jørgen Marvik ,  Kjartan Sandnes

IPC: B01F9/02 ,  B01F9/00 ,  B01F9/06 ,  C12M1/40 ,  C12M3/04 ,  B01F7/00 ,  B01F9/16

Abstract: A rotating drum apparatus for the mixing and processing of materials, the rotating drum apparatus comprising: a rotating drum (12) arranged with the length of the drum and the axis of rotation of the drum extending along the horizontal; an inlet at a first point on the drum (12) for receiving materials prior to mixing and/or processing; a screw (14) within the drum (12) for mixing the materials whilst conveying them lengthwise along the drum (12), wherein the screw (14) includes a helical blade extending along the length of the drum (12) with the outer edge of the helical blade being fixed to the inner surface of the drum (12) such that material can be conveyed and mixed in separated volumes (16) between each turn of the screw blade (14); an outlet at a second point along the drum for discharge of materials after mixing and/or processing; and a plurality mixing devices (18) for promoting mixing of the material in each of the separated volumes (16) of material as the material is conveyed along the screw (14), wherein the plurality of mixing devices (18) are spaced apart along the blade of the screw (14), and wherein there is at least one mixing device (18) for each turn of the screw blade (14).

公开(公告)号：US11072770B2

公开(公告)日：2021-07-27

申请号：US15517242

申请日：2015-10-05

Applicant: NUAS TECNOLOGY AS

Inventor： Lars Aglen

IPC: C12M1/40 ,  C12M1/12 ,  C12M1/00 ,  C12M1/02 ,  C12M1/04

Abstract: Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.

公开(公告)号：US20210115430A1

公开(公告)日：2021-04-22

申请号：US16613257

申请日：2018-05-16

Applicant: Ronald WOOD ,  The Curators of the University of Missouri

Inventor： Ronald Wood ,  Hsin-Yeh HSIEH ,  Chung-Ho Lin ,  George Stewart ,  Mason Schellenberg ,  Kattesh Katti ,  Sagar Gupta ,  Shibu Jose

IPC: C12N11/089 ,  C12N11/14 ,  C12M1/40 ,  C12P19/14 ,  A61K9/70 ,  C12P19/02 ,  C12P7/64 ,  A61K38/48 ,  A61K47/42

Abstract: Immobilized enzyme complexes (IEC) with enzymes that are non-covalently linked to matrices are provided along with methods for making the same. Methods of using the IEC for a wide variety of industrial enzymatic processes are also provided. Methods of converting cellulosic biomass and methods of effecting blood type conversions with the IEC are amongst the methods disclosed.

公开(公告)号：US20210062179A1

公开(公告)日：2021-03-04

申请号：US17073647

申请日：2020-10-19

Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY

Inventor： Sadagopan KRISHNAN ,  Charuksha Thameera WALGAMA ,  Rajasekhara Reddy NERIMETLA

IPC: C12N11/14 ,  C12M1/40 ,  C12M1/42 ,  C12P33/06

Abstract: Reusable microsomal biocatalytic systems (bioreactors) constructed on carbon nanostructure modified electrodes are provided. The bioreactors comprise stable, biologically active immobilized enzymes such as human cytochromes P 450 (CYPs) and their redox partner proteins, e.g. CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPR), on the carbon nanostructure surface. The immobilized enzymes may be present in liver microsomes, such as human liver microsomes (HLM) or as bactosomes, S9 fractions, etc. The bioreactors are used, for example, for synthesizing metabolites of interest from compounds such as drugs that are catabolized by the enzymes.

公开(公告)号：US10683480B2

公开(公告)日：2020-06-16

申请号：US15413201

申请日：2017-01-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: C40B60/14 ,  C12M1/00 ,  G01N1/28 ,  C12M1/40 ,  C12M3/00 ,  B01L3/00 ,  C12M1/33

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

公开(公告)号：US10655153B2

公开(公告)日：2020-05-19

申请号：US15921442

申请日：2018-03-14

Applicant: Timothy A. Wencewicz ,  Jason E. Schaffer ,  Margaret R. Reck

Inventor： Timothy A. Wencewicz ,  Jason E. Schaffer ,  Margaret R. Reck

IPC: C12P17/02 ,  C12P17/08 ,  C12N9/10 ,  C12M1/40 ,  C12M1/00

Abstract: Methods of producing peptide beta-lactones and beta-hydroxy acids are disclosed that include contacting a beta-hydroxy-alpha-amino acid, an aryl carrier protein (ObiD), and ATP with a non-ribosomal protein synthetase. A continuous flow reactor is disclosed that includes an elongate conduit with at least one region that includes a first region with a non-ribosomal protein synthetase immobilized to a substrate. The non-ribosomal protein synthetase of the continuous flow reactor is configured to contact a flow of a reaction mixture that includes a beta-hydroxy-alpha-amino acid and an aryl carrier protein. The non-ribosomal protein synthetase is further configured to release a peptide beta-lactone into the flow of the reaction mixture.