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公开(公告)号:US20200216893A1
公开(公告)日:2020-07-09
申请号:US16824920
申请日:2020-03-20
Applicant: Life Technologies Corporation
Inventor: Phil Waggoner , James A. Ball , Wolfgang Hinz , Michael L. Minto , Scott Parker , David M. Cox , Alexander Mastroianni , Jeremy Gray , Marc Glazer , Kimberly Gorrell
IPC: C12Q1/6869 , G01N27/414
Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
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公开(公告)号:US10688429B2
公开(公告)日:2020-06-23
申请号:US14587976
申请日:2014-12-31
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jason D. Brown , Clinton C. Staheli , Nephi D. Jones
IPC: B01D46/00 , C12M1/00 , B01F3/04 , C12M1/12 , B01F7/16 , B01F7/18 , B01F7/00 , B01F15/00 , C12M1/06
Abstract: A filter assembly includes a casing having of a polymeric film and bounding a compartment, the casing having an inlet opening communicating with the compartment and a first outlet opening communicating with the compartment. A first filter includes a porous filter body through which gas can pass and has a pore size smaller than 1 μm, the first filter being coupled with the casing so that the filter body is at least partially disposed within compartment of the casing and so that gas passing through the compartment of the casing from the inlet opening to the first outlet opening must pass through the filter body.
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公开(公告)号:US10683539B2
公开(公告)日:2020-06-16
申请号:US15882789
申请日:2018-01-29
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Parul Angrish , Zhiwei Yang
IPC: C12Q1/68 , C12Q1/686 , C07D207/16 , C07C41/22 , C07C211/63 , C07C303/28 , C07D211/60 , C11D1/88 , C12N9/12 , C12N9/96
Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.
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公开(公告)号:US10676787B2
公开(公告)日:2020-06-09
申请号:US14880439
申请日:2015-10-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Benjamin Donnet
IPC: C12Q1/6869 , G16B25/00
Abstract: Embodiments disclose methods, systems, and computer-readable media for accelerated base calling of sequencing data. These methods may be adapted to accelerate sequence determination for data arising from a variety of different nucleic acid sequencing platforms. In various embodiments, configurable logic circuits such as FPGAs and GPUs may be adapted to perform raw signal processing, basecalling, and/or sequence determination operations providing further enhancements to the sequence analysis methods.
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195.
公开(公告)号:US10669515B2
公开(公告)日:2020-06-02
申请号:US15392906
申请日:2016-12-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Nephi D. Jones , Christopher D. Brau
Abstract: A fluid mixing system includes a container bounding a compartment and extending between a first end and an opposing second end. An elongated first drive line and second drive line are disposed within the compartment of the container and are rotatable therein. At least one tie extends between the first drive line and the second drive line so as to maintain at least a portion of the first drive line and the second drive line at lateral spaced apart positions within the compartment. An impeller or other mixing element can be coupled to the drive lines.
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公开(公告)号:US20200158684A1
公开(公告)日:2020-05-21
申请号:US16677662
申请日:2019-11-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Keith G. FIFE
IPC: G01N27/414 , G01N27/30
Abstract: An ion-sensitive circuit can include a charge accumulation device, to accumulate a plurality of charge packets as a function of an ion concentration of a fluid, and at least one control and readout transistor, to generate an output signal as a function of the accumulated plurality of charge packets, the output signal representing the ion concentration of the solution. The charge accumulation device can include a first charge control electrode above a first electrode semiconductor region, an electrically floating gate structure above a gate semiconductor region and below an ion-sensitive passivation surface, a second charge control electrode above a second electrode semiconductor region, and a drain diffusion region. The first control electrode can control entry of charge into a gate semiconductor region in response to a first control signal. The ion-sensitive passivation surface can be configured to receive the fluid. The second charge control electrode can control transmission of the plurality of charge packets out of the gate semiconductor region and into the drain diffusion region in response to a second control signal. The drain diffusion region can receive the plurality of charge packets from the gate semiconductor region via the second electrode semiconductor region.
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公开(公告)号:US10655175B2
公开(公告)日:2020-05-19
申请号:US16153898
申请日:2018-10-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Todd Rearick , Mark Milgrew , Jonathan Schultz , Chris Papalias , Kim Johnson
IPC: C12Q1/6874 , G16B30/00
Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.
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公开(公告)号:US20200149091A1
公开(公告)日:2020-05-14
申请号:US16741471
申请日:2020-01-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Chunmei LIU , Shoulian DONG , Linda WONG
IPC: C12Q1/686 , C12Q1/6851 , C12Q1/6806
Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.
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公开(公告)号:US20200132622A1
公开(公告)日:2020-04-30
申请号:US16677666
申请日:2019-11-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan ROTHBERG , Wolfgang HINZ , Kim JOHNSON , James BUSTILLO
IPC: G01N27/414 , G01N33/543 , C12Q1/6818 , H01L27/088 , H01L29/78 , C12Q1/6874
Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
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公开(公告)号:US10597642B2
公开(公告)日:2020-03-24
申请号:US15690945
申请日:2017-08-30
Applicant: Life Technologies Corporation
Inventor: Peter Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett Rosenblum , Michael Phelan
IPC: C12Q1/68 , C12N9/12 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Q1/6804 , C12Q1/6818
Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
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