公开(公告)号：US20010054580A1

公开(公告)日：2001-12-27

申请号：US09905338

申请日：2001-07-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael I. Watkins ,  Richard B. Edwards

IPC: G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  C02F001/48 ,  C12Q001/70 ,  B03C001/02 ,  B01D035/06 ,  H01L021/425

CPC classification number: G01N33/54326 ,  G01N33/537 ,  G01N33/54333 ,  G01N33/545 ,  G01N33/56994 ,  G01N33/582 ,  G01N33/6854 ,  Y10S435/973 ,  Y10T428/2982 ,  Y10T428/2984 ,  Y10T428/2987 ,  Y10T428/2989 ,  Y10T428/2991

Abstract: Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles are distinguishable from each other by characteristics that permit them to be differentiated into groups, each group carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other groups. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment. Assays are also disclosed for the simultaneous detection of antibodies of different classes and a common antigen specificity or of a common class and different antigen specificities. Each type is accomplished by immunological binding at the surfaces of two distinct solid phases in a sequential manner with dissociation of the binding and washing of the solid phase in between the binding steps.

公开(公告)号：US20250168526A1

公开(公告)日：2025-05-22

申请号：US18943873

申请日：2024-11-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Evan Paul Thrush ,  Kevin Alan McDonald ,  Keith V. Kotchou

IPC: H04N25/633 ,  H04N25/46

Abstract: An imaging system dynamically computes an offset for an image based on an active region and a reference region of an image sensor used to generate the image. The imaging system applies the offset to pixels of the image. The imaging system further processes the image by performing flat-fielding, binning, or both.

公开(公告)号：US20250166749A1

公开(公告)日：2025-05-22

申请号：US18952032

申请日：2024-11-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jasmina DESAI ,  Shakila POTHINI

IPC: G16H10/40 ,  G16H40/67

Abstract: Systems and methods for automating hands-free workflow of laboratory procedures. The system includes a wearable device with a display and a workflow controller in communication with the wearable device. The workflow controller can be configured to extract a workflow for a specified laboratory procedure from a workflow repository. The workflow can include a plurality of instructions arranged in a specified sequence. One or more instructions of the plurality of instructions can be sequential delivered to the wearable device for an operator to follow. The one or more instructions can include a visual indicator configured to be displayed within the display of the wearable device. The wearable device can be configured to receive voice commands and the workflow controller can be configured to navigate the workflow based on the voice commands. The wearable device can be used to scan codes and display additional information during workflow implementation.

公开(公告)号：US12290813B2

公开(公告)日：2025-05-06

申请号：US17330663

申请日：2021-05-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky ,  Josh Shinoff

IPC: C12Q1/00 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806

Abstract: Methods and systems for sample target molecules are provided.

公开(公告)号：US20250137039A1

公开(公告)日：2025-05-01

申请号：US18952433

申请日：2024-11-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY

IPC: C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US20250129413A1

公开(公告)日：2025-04-24

申请号：US18983065

申请日：2024-12-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sean CATER ,  Lucas FRENZ ,  Andrew KOHLWAY ,  Jennifer CHEW ,  Rob RAY ,  Ronald LEBOFSKY

IPC: C12Q1/6853 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods of assessing whether multiple differently-barcodes primers are in partitions are provided.

公开(公告)号：US12275989B2

公开(公告)日：2025-04-15

申请号：US18386975

申请日：2023-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi Xue ,  Joshua Ritchey ,  Robert Meltzer

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6816

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US20250101391A1

公开(公告)日：2025-03-27

申请号：US18974072

申请日：2024-12-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12 ,  C12N1/20 ,  C12R1/01 ,  C40B40/06

Abstract: Hybrid reverse transcriptases are provided that comprise a non-retroviral retrotransposon, or a fragment of the non-retroviral retrotransposon having reverse transcriptase activity, joined to a nucleic acid binding protein. Also provided are methods of using the hybrid reverse transcriptases to prepare a cDNA molecule library.

公开(公告)号：US12241116B2

公开(公告)日：2025-03-04

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US12239987B2

公开(公告)日：2025-03-04

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: B01L3/00 ,  B01F23/41 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1434

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.