公开(公告)号：US09600679B2

公开(公告)日：2017-03-21

申请号：US13458369

申请日：2012-04-27

Applicant: William Calero ,  Glen Davis ,  Richard Brian Fowler ,  Richard Lindstedt ,  Umadevi Santhanam ,  Kalyanasundaram Subramanivan ,  Krishna Mitter ,  Kalidas Balakrishnan ,  Mahabaleshwar Mallappa Asundi ,  Ramesh Sunder ,  Nithya Balachandran ,  Praveen Kumar Sivapuram ,  Anil Kumar ,  Mandhakini Subramanian

Inventor： William Calero ,  Glen Davis ,  Richard Brian Fowler ,  Richard Lindstedt ,  Umadevi Santhanam ,  Kalyanasundaram Subramanivan ,  Krishna Mitter ,  Kalidas Balakrishnan ,  Mahabaleshwar Mallappa Asundi ,  Ramesh Sunder ,  Nithya Balachandran ,  Praveen Kumar Sivapuram ,  Anil Kumar ,  Mandhakini Subramanian

IPC: G06F7/04 ,  G06F15/16 ,  G06F17/30 ,  H04L29/06 ,  G06F21/62

CPC classification number: G06F21/6218 ,  G06F2221/2141 ,  G06F2221/2147 ,  H04L63/0884

Abstract: Techniques for resource operation based on usage, sharing, and recommendations with modular authentication are provided. A resource space is associated with a principal. The resource space comprises resources local to a device of the principal and remote from the device. The resources presented in a merged view within a local file system and operating system of the device. In an embodiment, the device negotiates authentication with an intermediary for access to a legacy service where authentication is performed by the intermediary on behalf of the device, and the intermediary establishes an authentication session between the principal and the legacy service.

公开(公告)号：US20120021523A1

公开(公告)日：2012-01-26

申请号：US13168041

申请日：2011-06-24

Applicant: Brian Fowler ,  Andrew May

Inventor： Brian Fowler ,  Andrew May

IPC: G01N23/20

CPC classification number: G01N23/20025

Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

公开(公告)号：US08058630B2

公开(公告)日：2011-11-15

申请号：US12688462

申请日：2010-01-15

Applicant: Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: G01N21/64

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20090257920A1

公开(公告)日：2009-10-15

申请号：US12422612

申请日：2009-04-13

Applicant: Geoffrey Facer ,  Brian Fowler ,  Emerson Cheung Quan ,  Marc Unger

Inventor： Geoffrey Facer ,  Brian Fowler ,  Emerson Cheung Quan ,  Marc Unger

IPC: B01L3/00 ,  G05D11/02

CPC classification number: F16K99/0015 ,  B01F5/02 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  B33Y80/00 ,  C12Q1/686 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2021/0346 ,  Y10T137/0329 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2559 ,  Y10T137/2655 ,  Y10T137/2688 ,  Y10T137/87249

Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.

Abstract translation: 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。

公开(公告)号：US20090147918A1

公开(公告)日：2009-06-11

申请号：US12118185

申请日：2008-05-09

Applicant: Brian Fowler ,  Andrew May

Inventor： Brian Fowler ,  Andrew May

IPC: G01N23/207

CPC classification number: G01N23/20025

Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

公开(公告)号：US20080109553A1

公开(公告)日：2008-05-08

申请号：US11595787

申请日：2006-11-08

Applicant: Brian Fowler

Inventor： Brian Fowler

IPC: G06F15/16

CPC classification number: G06Q30/02

Abstract: The present invention provides a way to reduce click fraud by verifying that a human user is making URI requests. In a preferred embodiment, the present invention comprises a client-side plug-in or other suitable component adapted to: (a) assemble a list of enabled domains; (b) when a user first requests a resource from an enabled domain, modify hyperlinks in the response that target resources in any enabled domain; and (c) when a user attempts to follow a modified hyperlink, present a challenge to the user that the user must successfully negotiate before the user is granted access to the resource targeted by the hyperlink.

Abstract translation: 本发明提供了一种通过验证人类用户正在进行URI请求来减少点击欺诈的方法。 在优选实施例中，本发明包括客户端插件或其他适合的组件，其适于：（a）组合启用域的列表; （b）当用户首先从启用的域请求资源时，修改在任何启用的域中的目标资源的响应中的超链接; 和（c）当用户尝试遵循经修改的超链接时，在用户被授予对超链接所针对的资源的访问之前向用户呈现用户必须成功协商的挑战。

公开(公告)号：US06999458B2

公开(公告)日：2006-02-14

申请号：US10366372

申请日：2003-02-14

Applicant: Brian Fowler

Inventor： Brian Fowler

IPC: H04L12/28

CPC classification number: H04L61/157 ,  H04L29/06027 ,  H04L29/12018 ,  H04L29/1216 ,  H04L61/10 ,  H04L65/103 ,  H04L65/104 ,  H04L65/1069 ,  H04M7/128 ,  H04M15/56 ,  H04M2215/202

Abstract: A telephony network for enabling the origination and termination of telephone calls between one or more subscriber terminals connected to a public packet network with an open addressing protocol and one or more non-subscriber terminals connected to a PSTN network. Subscribers to the telephony network have E.164 numbers mapped to their current IP address and published by a mapping server so other subscribers and non-subscribers can call the subscriber. The mapped information is published on either a HTML or XML page for direct use over the Internet by other subscribers or for use by a gateway device. The gateway device enables non-subscribers on the PSTN to directly call subscribers. Calls between subscribers completely by-pass the PSTN. Subscribers can also call anyone with a phone number whether they are a subscriber or not.

Abstract translation: 一种电话网络，用于使连接到具有开放寻址协议的公共分组网络的一个或多个用户终端和连接到PSTN网络的一个或多个非用户终端之间的电话呼叫的发起和终止。 电话网络的订户具有映射到其当前IP地址并由映射服务器发布的E.164号码，因此其他用户和非订户可以呼叫用户。 映射的信息在HTML或XML页面上发布，供其他用户在Internet上直接使用或由网关设备使用。 网关设备使得PSTN上的非用户能够直接呼叫用户。 用户之间的呼叫完全绕过PSTN。 用户也可以拨打电话号码的任何人，无论他们是否是订户。

公开(公告)号：US5987322A

公开(公告)日：1999-11-16

申请号：US835156

申请日：1997-04-03

Applicant: Sudeep Gupta ,  Charles Brian Fowler

Inventor： Sudeep Gupta ,  Charles Brian Fowler

IPC: H04W8/24 ,  H04W48/12 ,  H04W48/16 ,  H04B1/00

CPC classification number: H04W48/16 ,  H04W48/12 ,  H04W8/245

Abstract: Each cell area within a mobile telecommunications service area is categorized in accordance with its associated terrestrial conditions and irregularities. As an illustration, each cell area is categorized as either a urban, suburban or rural area. Data indicative of the assigned category are then broadcast over a control channel to all mobile stations currently traveling within the respective cell area. The mobile stations then utilize the received data to determine appropriate air-interface settings and parameters and effectuate location adaptive air-interfaces with a serving mobile telecommunications network.

Abstract translation: 移动电信服务区域内的每个小区区域根据其相关的地面条件和不规则性进行分类。 作为示例，每个细胞区域被分类为城市，郊区或农村地区。 然后，通过控制信道将指示分配类别的数据广播到当前在相应小区区域内行进的所有移动台。 然后，移动站利用所接收的数据来确定适当的空中接口设置和参数，并且利用服务的移动电信网络来实现位置自适应空中接口。

公开(公告)号：US09074204B2

公开(公告)日：2015-07-07

申请号：US13476911

申请日：2012-05-21

Applicant: Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC: C12Q1/68 ,  C12N15/10 ,  C12N15/65 ,  C12N15/66

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

公开(公告)号：US09039997B2

公开(公告)日：2015-05-26

申请号：US13499879

申请日：2010-09-29

Applicant: David Cohen ,  Andrew May ,  Martin Pieprzyk ,  Brian Fowler ,  Kim Huat Lee ,  Jun Yan ,  Ming Fang Zhou ,  Seng Beng Ng

Inventor： David Cohen ,  Andrew May ,  Martin Pieprzyk ,  Brian Fowler ,  Kim Huat Lee ,  Jun Yan ,  Ming Fang Zhou ,  Seng Beng Ng

IPC: B01L99/00 ,  B01L3/00 ,  B01L9/00 ,  B01L7/00 ,  G01N1/40

CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

Abstract translation: 本发明包括具有微型空腔的​​微流体系统，其可以被可移除的盖覆盖，其中可移除的盖允许微加工腔的至少一部分开口被操作者暴露或直接访问。 微流体系统包括在可包括PDMS的弹性体层中形成的腔室，流动和控制通道。 可移除的盖包括通过粘合剂层结合到弹性体层的热塑性基底膜。 当可移除的盖被剥离时，室至少部分地打开以允许从室中取样。 该室可能在PCR反应内部形成大分子晶体或产生内含物。 本发明还包括通过使用可移除盖制造弹性体层中的通孔的方法。 本发明还包括用于剥离可剥离盖或诸如集成式加热器撒布机的可移除部件的方法和装置。