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    HIGH THROUGHPUT GENOME SEQUENCING ON DNA ARRAYS
    23.
    发明申请
    HIGH THROUGHPUT GENOME SEQUENCING ON DNA ARRAYS 审中-公开

    公开(公告)号：US20190010542A1

    公开(公告)日：2019-01-10

    申请号：US15940771

    申请日：2018-03-29

    Applicant: Complete Genomics Inc.

    Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac

    IPC: C12Q1/6874 ,  C12N15/66 ,  C12N15/64

    Abstract: The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

    Preparing a DNA fragment library for sequencing using tagged primers
    24.
    发明授权
    Preparing a DNA fragment library for sequencing using tagged primers 有权

    公开(公告)号：US10125392B2

    公开(公告)日：2018-11-13

    申请号：US13971797

    申请日：2013-08-20

    Applicant: Complete Genomics, Inc.

    Inventor： Radoje Drmanac

    IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14 ,  C12Q1/6806

    Abstract: The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

    Flow cells for high density array chips
    29.
    发明授权
    Flow cells for high density array chips 有权

    公开(公告)号：US09803239B2

    公开(公告)日：2017-10-31

    申请号：US13840482

    申请日：2013-03-15

    Applicant: Complete Genomics, Inc.

    Inventor： Bill J. Peck ,  Mark Fuller ,  Daniel West ,  Anthony Delacruz

    IPC: B01L3/00 ,  C12Q1/68 ,  G01N21/05

    CPC classification number: C12Q1/6874 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/565 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  G01N21/05

    Abstract: Biochemical flow cells having sealed inlets and outlets are provided for performing high-volume assays on macromolecules. In one example embodiment, a flow cell with detachable inlet and outlet connectors comprises an inlet manifold, a coverslip, and a substrate disposed below the coverslip to form a reaction chamber, where the substrate is disposed to partially cover the inlet manifold such that a slit is formed along an entire edge of the substrate where fluids can flow from the inlet manifold through the slit, around substantially the entire edge of the substrate, and into the reaction chamber at equalized pressure and without bubbles. In another embodiment, a flow cell comprises an outlet manifold, two or more flow regions each connected to its own loading port via its own flow distribution funnel, each loading port connected to the outlet manifold, and plugs in a wall of the outlet manifold opposite each loading port, such that when a plug is absent from the wall of the outlet manifold, a loading tip may be inserted in its place, passing through the outlet manifold and connecting directly to a loading port.

    High Throughput Genome Sequencing on DNA Arrays
    30.
    发明申请
    High Throughput Genome Sequencing on DNA Arrays 审中-公开

    公开(公告)号：US20170226577A1

    公开(公告)日：2017-08-10

    申请号：US15267514

    申请日：2016-09-16

    Applicant: Complete Genomics Inc.

    Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12N15/64 ,  C12N15/66 ,  Y10T436/143333 ,  C12Q2521/313 ,  C12Q2525/191 ,  C12Q2525/151 ,  C12Q2525/131 ,  C12Q2565/518 ,  C12Q2533/107 ,  C12Q2531/125

    Abstract: The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

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