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21.发明授权Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof 有权具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US08241878B2
公开(公告)日：2012-08-14
申请号：US13296944
申请日：2011-11-15
申请人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
发明人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
IPC分类号： C12P7/58
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20110313206A1
公开(公告)日：2011-12-22
申请号：US12966333
申请日：2010-12-13
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
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公开(公告)号：US20090203097A1
公开(公告)日：2009-08-13
申请号：US12330534
申请日：2008-12-09
CPC分类号： C12P7/16 , C12N9/1007 , Y02E50/10
摘要： Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol.
摘要翻译： 筛选不是天然丁醇生产者的脂肪酸饲养细菌增加膜环丙烷脂肪酸提供改善的丁醇耐受性。 在酶底物存在下增加环丙烷脂肪酸合酶的表达，该酶底物是细胞内源的或进入细胞，提高了丁醇耐受性。 具有增加的环丙烷脂肪酸合成酶并具有丁醇生物合成途径的细菌菌株可用于生产丁醇。
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公开(公告)号：US20080274526A1
公开(公告)日：2008-11-06
申请号：US12110526
申请日：2008-04-28
申请人： Michael G. Bramucci , Dennis Flint , Edward S. Miller , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
发明人： Michael G. Bramucci , Dennis Flint , Edward S. Miller , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1022 , C12N9/1096 , C12N9/88 , Y02E50/10
摘要： A method for the production of isobutanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
摘要翻译： 公开了通过使用微生物生产宿主发酵生产异丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。
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公开(公告)号：US20080274524A1
公开(公告)日：2008-11-06
申请号：US12110503
申请日：2008-04-28
申请人： MICHAEL G. BRAMUCCI , Dennis Flint , Edward S. Miller , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
发明人： MICHAEL G. BRAMUCCI , Dennis Flint , Edward S. Miller , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
IPC分类号： C12P7/16
CPC分类号： C12N15/74 , C12N15/70 , C12P7/16 , C12Y103/01038 , C12Y103/01044 , C12Y402/01017 , C12Y402/01055 , Y02E50/10
摘要： A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
摘要翻译： 公开了通过使用微生物生产宿主发酵生产1-丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。
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公开(公告)号：US20080096276A1
公开(公告)日：2008-04-24
申请号：US11843676
申请日：2007-08-23
申请人： David Hallahan , Christopher Coldren , Dennis Flint , Hong Wang
发明人： David Hallahan , Christopher Coldren , Dennis Flint , Hong Wang
CPC分类号： C12N9/1085 , C12N15/8243 , C12N15/8279
摘要： This invention pertains to nucleic acid fragments encoding plant proteins that are homologs to the cis-prenyltransferases UPP synthase from the bacterium Micrococcus luteus or Dedol-PP synthase from yeast Saccharomyces cerevisiae. More specifically, this invention pertains to cis-prenyltransferase homologs from wheat, grape, soybean, rice, African daisy, rubber tree latex and pot marigold.
摘要翻译： 本发明涉及编码植物蛋白质的核酸片段，其与来自酵母酿酒酵母的细菌微球菌或Dedol-PP合酶的顺式 - 异戊烯基转移酶UPP合酶的同系物。 更具体地说，本发明涉及来自小麦，葡萄，大豆，大米，非洲雏菊，橡胶树乳胶和万寿菊的顺式 - 异戊烯基转移酶同源物。
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公开(公告)号：US20070092957A1
公开(公告)日：2007-04-26
申请号：US11586315
申请日：2006-10-25
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
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28.发明授权Strain for butanol production with increased membrane unsaturated trans fatty acids 有权用不饱和反式脂肪酸增加的丁醇生产菌株公开(公告)号：US08652823B2
公开(公告)日：2014-02-18
申请号：US12625593
申请日：2009-11-25
申请人： Dennis Flint , Tina K. Van Dyk
发明人： Dennis Flint , Tina K. Van Dyk
摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.
摘要翻译： 发现当不饱和反式脂肪酸的膜含量增加时，不是天然丁醇生产者的细菌具有增加的对丁醇的耐受性。 用不饱和反式脂肪酸喂养细胞增加其在膜中的浓度，这也可以通过表达脂肪酸顺反异构酶来实现。
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公开(公告)号：US20120064561A1
公开(公告)日：2012-03-15
申请号：US13029558
申请日：2011-02-17
申请人： DENNIS FLINT , Brian James Paul , Rick W. Ye
发明人： DENNIS FLINT , Brian James Paul , Rick W. Ye
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12N15/81 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , C12Y402/01009 , G01N33/573 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
摘要翻译： 本发明涉及包含二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。 本发明还涉及由于多肽的表达增加，细胞的Fe-S簇生物合成的调节或其组合而导致的具有增加的二羟基酸脱水酶多肽的比活性的重组宿主细胞。 本发明还包括使用宿主细胞的方法，以及用于鉴定增加宿主细胞中Fe-S簇生物合成途径中的通量的多肽的方法。
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公开(公告)号：US07993889B1
公开(公告)日：2011-08-09
申请号：US12018216
申请日：2008-01-23
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
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