公开(公告)号：US20090305326A1

公开(公告)日：2009-12-10

申请号：US12135629

申请日：2008-06-09

Applicant: David J. Beebe ,  Jay W. Warrick

Inventor： David J. Beebe ,  Jay W. Warrick

IPC: C12Q1/02 ,  B01J19/00 ,  C12N5/08

CPC classification number: B01J19/0093 ,  B01J2219/00804 ,  B01J2219/00833 ,  C12M23/16 ,  C12M29/10 ,  C12M35/08 ,  Y10T29/49826 ,  Y10T436/11 ,  Y10T436/2575

Abstract: A microfluidic device and method is provided for coupling discrete channels and for co-culture. The microfluidic device includes first and second bodies. Each body has a bottom surface and defines a channel. The channel in each body includes an inlet and an outlet communicating with the bottom surface. A first fluid, such as a first cell suspension, is provided within the channel of the first body and a second fluid, such a second cell suspension, is provided within the channel of the second body. The first and second bodies are movable between a first position wherein the outlet of the channel of the first body is spaced from the inlet of the channel of the second body and a second position wherein the fluid at the outlet of the channel of the first body communicates with the fluid at the inlet of the channel of the second body.

Abstract translation: 提供微流体装置和方法用于耦合离散通道并用于共培养。 微流体装置包括第一和第二体。 每个身体都有一个底部表面，并定义一个通道。 每个主体中的通道包括与底面连通的入口和出口。 第一流体，例如第一细胞悬浮液，设置在第一体的通道内，第二流体，例如第二细胞悬浮液，设置在第二体的通道内。 第一和第二主体可在第一位置和第二位置之间移动，第一位置的第一主体的通道的出口与第二主体的通道的入口间隔开，而第二位置，其中第一主体的通道出口处的流体 与第二主体的通道入口处的流体连通。

公开(公告)号：US07562562B2

公开(公告)日：2009-07-21

申请号：US11745855

申请日：2007-05-08

Applicant: David J. Beebe ,  Dongshin Kim

Inventor： David J. Beebe ,  Dongshin Kim

IPC: G01N33/48 ,  G01N3/08

CPC classification number: G01N19/00 ,  G01N19/10 ,  G01N33/48

Abstract: A method and device is provided for detecting a predetermined material. The device includes a first inner layer having first and second surfaces and a volume responsive to a first predetermined stimuli. A second inner layer has first and second surfaces. An adhesive bonds at least a portion of the first surface of the first inner layer to the first surface of the second inner layer with a bonding force. A change in the volume of the first layer generates an elastic force on the adhesive material. As a result, the first inner layer delaminates from the second inner layer in response to the elastic force overcoming the bonding force.

Abstract translation: 提供了用于检测预定材料的方法和装置。 该装置包括具有第一和第二表面的第一内层和响应于第一预定刺激的体积。 第二内层具有第一和第二表面。 粘合剂通过结合力将第一内层的第一表面的至少一部分粘合到第二内层的第一表面。 第一层的体积变化在粘合剂材料上产生弹性力。 结果，第一内层响应于克服接合力的弹力而从第二内层分层。

公开(公告)号：US20090155840A1

公开(公告)日：2009-06-18

申请号：US11958028

申请日：2007-12-17

Applicant: David J. Beebe ,  Nisha A. McConnell ,  Hongmei Yu

Inventor： David J. Beebe ,  Nisha A. McConnell ,  Hongmei Yu

IPC: C12Q1/06 ,  C12M1/00

CPC classification number: G01N33/5005 ,  B01L3/502761 ,  G01N15/1484 ,  G01N2015/1486

Abstract: A microfluidic device and method is provided for determining a cell concentration in a sample. The microfluidic device includes a body having a channel therethough that extends along an axis. The channel includes an input and an output, and is at least partially defined by a surface. Indicia overlaps the surface. The channel has a predetermined volume. A portion of the sample is provided in the channel and the cells in the predetermined portions of the channel defined by the indicia are counted.

Abstract translation: 提供微流体装置和方法来确定样品中的细胞浓度。 微流体装置包括具有沿轴线延伸的通道的主体。 通道包括输入和输出，并且至少部分地由表面限定。 标记与表面重叠。 通道具有预定的音量。 样本的一部分被提供在通道中，并且由标记限定的通道的预定部分中的单元被计数。

公开(公告)号：US20080276718A1

公开(公告)日：2008-11-13

申请号：US11745855

申请日：2007-05-08

Applicant: David J. Beebe ,  Dongshin Kim

Inventor： David J. Beebe ,  Dongshin Kim

IPC: G01N3/08

CPC classification number: G01N19/00 ,  G01N19/10 ,  G01N33/48

Abstract: A method and device is provided for detecting a predetermined material. The device includes a first inner layer having first and second surfaces and a volume responsive to a first predetermined stimuli. A second inner layer has first and second surfaces. An adhesive bonds at least a portion of the first surface of the first inner layer to the first surface of the second inner layer with a bonding force. A change in the volume of the first layer generates an elastic force on the adhesive material. As a result, the first inner layer delaminates from the second inner layer in response to the elastic force overcoming the bonding force.

Abstract translation: 提供了用于检测预定材料的方法和装置。 该装置包括具有第一和第二表面的第一内层和响应于第一预定刺激的体积。 第二内层具有第一和第二表面。 粘合剂通过结合力将第一内层的第一表面的至少一部分粘合到第二内层的第一表面。 第一层的体积变化在粘合剂材料上产生弹性力。 结果，第一内层响应于克服接合力的弹力而从第二内层分层。

公开(公告)号：US07277021B2

公开(公告)日：2007-10-02

申请号：US11033370

申请日：2005-01-11

Applicant: David J. Beebe ,  Brian E. Schwartz ,  Steven J. Pauls ,  Timothy D. Rand ,  Brant R. Kochsiek

Inventor： David J. Beebe ,  Brian E. Schwartz ,  Steven J. Pauls ,  Timothy D. Rand ,  Brant R. Kochsiek

IPC: G08B19/00 ,  G08B21/00 ,  G08B23/00 ,  G01M19/00 ,  G01L1/22 ,  A43B5/00 ,  A43B23/00

CPC classification number: A43B3/001 ,  A43B3/0005 ,  A43B5/00 ,  A43B7/00 ,  G01N3/56

Abstract: A device and method are provided for determining the wear of a sole of a shoe. The device includes first and second sensors receivable in the sole of the shoe. The sensors are axially spaced and generate signals in response to corresponding impact forces acting thereon. A control circuit is connectable to the first and second sensors. The control circuit compares the difference between the first and second signals to a threshold and generates an alert signal in response to the difference between the first and second signal meeting the threshold.

Abstract translation: 提供了一种用于确定鞋底的磨损的装置和方法。 该装置包括可容纳在鞋底中的第一和第二传感器。 传感器轴向间隔开并响应于作用于其上的相应冲击力产生信号。 控制电路可连接到第一和第二传感器。 控制电路将第一和第二信号之间的差异与阈值进行比较，并且响应于满足阈值的第一和第二信号之间的差异而产生报警信号。

公开(公告)号：US07189580B2

公开(公告)日：2007-03-13

申请号：US10271488

申请日：2002-10-16

Applicant: David J. Beebe ,  Glenn M. Walker

Inventor： David J. Beebe ,  Glenn M. Walker

IPC: G01N1/10 ,  G01N1/00 ,  B01L3/02 ,  B32B5/02 ,  B32B27/04

CPC classification number: B01L3/50273 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  Y10T137/0357 ,  Y10T137/0363 ,  Y10T137/0379 ,  Y10T137/0396 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A method is provided for pumping fluid through a channel of a microfluidic device. The channel has an input port of a predetermined radius and an output port of a predetermined radius. The channel is filled with fluid and a pressure gradient is generated between the fluid between the input port and the fluid at the output port. As a result, fluid flows through the channel towards the output port.

Abstract translation: 提供了一种用于泵送流体通过微流体装置的通道的方法。 通道具有预定半径的输入端口和预定半径的输出端口。 通道填充有流体，并且在输出端口和输出端口处的流体之间的流体之间产生压力梯度。 结果，流体流过通道朝向输出端口。

公开(公告)号：US06821475B2

公开(公告)日：2004-11-23

申请号：US10125498

申请日：2002-04-17

Applicant: David J. Beebe ,  Joseph M. Bauer

Inventor： David J. Beebe ,  Joseph M. Bauer

IPC: B29C3902

CPC classification number: B29C59/026 ,  B29C59/022 ,  B29C2059/023 ,  Y10S264/44

Abstract: A method of fabricating a microstructure is provided. The method includes the step of providing a layer of a polyermizable material. A solid is brought into contact with the layer of polymerizable material so as to alter the shape of the upper surface of the layer. Thereafter, the layer of polymerizable material is polymerized such that the layer solidifies and the upper surface thereof assumes a desired three-dimensional configuration.

Abstract translation: 提供了一种制造微结构的方法。 该方法包括提供多层可熔化材料层的步骤。 使固体与可聚合材料层接触，以改变层的上表面的形状。 此后，聚合材料层使得层固化，并且其上表面呈现所需的三维构型。

公开(公告)号：US08888757B2

公开(公告)日：2014-11-18

申请号：US12473028

申请日：2009-05-27

Applicant: Benjamin J. Moga ,  David J. Beebe

Inventor： Benjamin J. Moga ,  David J. Beebe

IPC: A61M31/00 ,  A61B17/20 ,  A61M5/00 ,  A61M25/00 ,  A61M5/32 ,  A61M37/00

CPC classification number: A61M37/0015 ,  A61M2037/0046

Abstract: An active microneedle array and method are provided for penetrating an outer layer of an epidermis. The active microneedle array includes a base having first and second sides. The first side of the base is engageable with the epidermis. A microneedle projects from the first side of the base. The microneedle is moveable between a first initial configuration and a second deformed configuration in response to engagement with the epidermis so as to form a passageway therein.

Abstract translation: 提供活性微针阵列和方法用于穿透表皮的外层。 活性微针阵列包括具有第一和第二侧面的基底。 基部的第一侧可与表皮接触。 一根微针从基地的第一侧突出。 响应于与表皮的接合，微针可以在第一初始构型和第二变形构型之间移动，以在其中形成通道。

公开(公告)号：US08790931B2

公开(公告)日：2014-07-29

申请号：US12753290

申请日：2010-04-02

Applicant: David J. Beebe ,  Jay Warrick ,  Erwin Berthier

Inventor： David J. Beebe ,  Jay Warrick ,  Erwin Berthier

IPC: G01N1/40 ,  C12Q1/24 ,  B01L3/00 ,  G01N1/38

CPC classification number: G01N1/4077 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0803 ,  B01L2300/0809 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/24 ,  G01N2001/383 ,  G01N2001/4083 ,  Y10T436/25 ,  Y10T436/25375

Abstract: A method is provided for collecting a concentration of particles from a sample fluid containing the particles. The method includes the steps of providing a microfluidic device. The microfluidic device includes an input channel, an output channel and a collection region. The input channel has an input end and an output end. The output channel has an input end and an output end. The collection region interconnects the output end of the input channel and the input end of the output channel. The sample fluid flows through the input channel and the output channel at a first velocity and through the collection region at a second velocity less than the first velocity such that the particles collect in therein.

Abstract translation: 提供了一种从含有颗粒的样品流中收集颗粒浓度的方法。 该方法包括提供微流体装置的步骤。 微流体装置包括输入通道，输出通道和收集区域。 输入通道具有输入端和输出端。 输出通道具有输入端和输出端。 收集区域将输入通道的输出端和输出通道的输入端互连。 样品流体以第一速度流过输入通道和输出通道，并以小于第一速度的第二速度通过收集区域，使得颗粒收集在其中。

公开(公告)号：US20130276890A1

公开(公告)日：2013-10-24

申请号：US13450759

申请日：2012-04-19

Applicant: David J. Beebe ,  Jay Warrick ,  Erwin Barthler

Inventor： David J. Beebe ,  Jay Warrick ,  Erwin Barthler

IPC: F16L41/00 ,  F17D3/00

CPC classification number: F17D3/00 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0688 ,  B01L2400/084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: A device and method of laminar flow patterning of at least one sample fluid in a main channel in a microfluidic device are provided. A first input channel is provided in the microfluidic device. The first input channel has an output end communicating with the first end of the main channel and an input end communicating with a first input port. A buffer fluid is deposited in the main channel and the first input channel and a first sample fluid is deposited in the first input port. A first pressure is generated in response to the depositing of the first sample fluid in the first input port so as to cause laminar flow of the first sample fluid in the main channel.

Abstract translation: 提供了在微流体装置中的主通道中的至少一个样品流体的层流图案化的装置和方法。 在微流体装置中提供第一输入通道。 第一输入通道具有与主通道的第一端通信的输出端和与第一输入端口通信的输入端。 缓冲流体沉积在主通道中，并且第一输入通道和第一样品流体沉积在第一输入端口中。 响应于第一样品流体在第一输入端口中的沉积而产生第一压力，以使第一样品流体在主通道中层流。