公开(公告)号：US08986629B2

公开(公告)日：2015-03-24

申请号：US13777879

申请日：2013-02-26

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J. A. Chen ,  David J. Fullagar

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/4473 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721 ,  Y10T436/11 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

Abstract translation: 公开了一种用于控制，检测和测量分子复合物的装置。 该装置包括公共电极。 该装置还包括多个测量单元。 每个测量单元包括电池耦合到电池电极的单元电极和积分器。 积分器测量在公共电极和电池电极之间流动的电流。 该装置还包括多个模数转换器，其中来自多个测量单元的积分器电耦合到多个模数转换器的一个模 - 数转换器。

公开(公告)号：US20140374252A1

公开(公告)日：2014-12-25

申请号：US14335616

申请日：2014-07-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68 ,  G01N33/487

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

Abstract translation: 本文描述了在脂质双层中形成纳米孔的技术。 在一个实例中，将搅动刺激水平（例如电刺激刺激）施加到脂质双层，其中搅动刺激水平倾向于促进脂质双层中的纳米孔的形成。 在一些实施方案中，检测由在脂质双层中形成纳米孔而导致的脂质双层的电性质的变化，并且基于检测到的脂质双层电性能的变化来确定在脂质双层中形成的纳米孔 。

公开(公告)号：US20140303032A1

公开(公告)日：2014-10-09

申请号：US14355203

申请日：2012-11-01

Applicant: GENIA TECHNOLOGIES, INC. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B82Y15/00 ,  C12Q1/6869 ,  C12Q2525/101 ,  C12Q2537/163 ,  C12Q2565/607 ,  C12Q2565/631 ,  C12Q2525/113 ,  C12Q2525/107

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

Abstract translation: 提供了多核苷酸测序的系统和方法。 系统和方法通过纳米孔或一类蛋白质或突变蛋白优化样品（例如，多核苷酸）的控制，速度，移动和/或易位，以便积累足够的时间和电流 阻断信息以鉴定多核苷酸的单链区域中的连续核苷酸或多个核苷酸。

公开(公告)号：US10456993B2

公开(公告)日：2019-10-29

申请号：US14335616

申请日：2014-07-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y10/00 ,  B82Y40/00

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20190217552A1

公开(公告)日：2019-07-18

申请号：US16179791

申请日：2018-11-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: B29C67/20 ,  C12Q1/6869 ,  B82Y5/00 ,  G01N33/487

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20190185927A1

公开(公告)日：2019-06-20

申请号：US16272970

申请日：2019-02-11

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  C12M1/34 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20190064141A1

公开(公告)日：2019-02-28

申请号：US16172633

申请日：2018-10-26

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20180340157A1

公开(公告)日：2018-11-29

申请号：US15989109

申请日：2018-05-24

Applicant: Genia Technologies, Inc.

Inventor： Helen FRANKLIN ,  Cynthia CECH ,  Timothy Kellogg CRAIG ,  Aruna AYER ,  Kirti DHIMAN ,  Natalie B. CHECHELSKI JOHNSTON ,  Joshua N. MABRY ,  Arkadiusz BIBILLO ,  Peter CRISALLI ,  Randall W. DAVIS

IPC: C12N9/12

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

公开(公告)号：US10036725B2

公开(公告)日：2018-07-31

申请号：US15288107

申请日：2016-10-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: G01N27/447 ,  G01N27/22 ,  G01N33/487

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

公开(公告)号：US10012637B2

公开(公告)日：2018-07-03

申请号：US15462483

申请日：2017-03-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  David J. Fullagar

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.