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公开(公告)号：US20080176313A1
公开(公告)日：2008-07-24
申请号：US11929880
申请日：2007-10-30
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C12Y304/21062
摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 变体蛋白酶一般通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。
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公开(公告)号：US20080145353A1
公开(公告)日：2008-06-19
申请号：US10581014
申请日：2004-12-03
申请人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译： 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗，漂白和消毒的应用中。
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公开(公告)号：US20080124783A1
公开(公告)日：2008-05-29
申请号：US11929907
申请日：2007-10-30
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C12Y304/21062
摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。
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公开(公告)号：US07157416B2
公开(公告)日：2007-01-02
申请号：US10333176
申请日：2001-07-20
申请人： Nathaniel T. Becker , Richard R. Bott , Shauna L. Bowden , Meng Hong Heng , Christian Paech , Antti V. Kosola
发明人： Nathaniel T. Becker , Richard R. Bott , Shauna L. Bowden , Meng Hong Heng , Christian Paech , Antti V. Kosola
CPC分类号： C11D7/10 , C11D3/38663 , C11D7/261 , C12N9/96
摘要： Enzyme-containing formulations having improved stability and enzymatic activity in liquid medium, comprising one or more protease enzymes produced from any Bacillus bacteria, at least about 5% alkali metal halide salt, and at least about 50% polyol. Also disclosed are methods for making such formulations.
摘要翻译： 含有液体培养基中具有改善的稳定性和酶活性的含酶制剂包含由任何芽孢杆菌属细菌产生的一种或多种蛋白酶，至少约5％的碱金属卤化物盐和至少约50％的多元醇。 还公开了制备这种制剂的方法。
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公开(公告)号：US06627744B2
公开(公告)日：2003-09-30
申请号：US09824827
申请日：2001-04-02
IPC分类号： C07H1700
CPC分类号： B82Y5/00 , A61K47/51 , A61K47/665 , A61K47/6898 , C07H5/10 , C07K14/36
摘要： The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine n a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins. The present invention also relates to synthetic methods for producing thio-glycoses, the thio-glycoses so produced, and to methods for producing glycodendrimer reagents.
摘要翻译： 本发明涉及一种化学修饰的突变蛋白，其包含半胱氨酸残基，其取代了半胱氨酸以外的残基而不是前体蛋白，随后通过半胱氨酸残基与糖基化硫代磺酸酯反应来修饰取代的半胱氨酸残基。 还提供了一种生产化学修饰的突变蛋白的方法。 本发明还涉及糖基化甲磺酸盐。 本发明的另一方面是一种改变蛋白质的功能特征的方法，包括提供蛋白质并使蛋白质与糖基化甲磺酸盐试剂在与蛋白质相比产生具有改变的功能特征的糖蛋白的条件下反应。 此外，本发明涉及确定化学修饰的突变蛋白的结构 - 功能关系的方法。 本发明还涉及用于制备硫代糖糖，如此制备的硫代糖糖的合成方法，以及制备糖苷配基体试剂的方法。
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26.发明授权Subtilisin variants multiply-substituted subtilising variants having a substitution at position 76 失效枯草杆菌蛋白酶变体在第76位具有取代的多取代的枯草芽孢杆菌变体公开(公告)号：US06586221B2
公开(公告)日：2003-07-01
申请号：US08322678
申请日：1994-10-13
IPC分类号： C12N950
CPC分类号： C11D3/38609 , C12N9/54
摘要： Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶变体。 变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体羰基水解酶具有与前体水解酶不同的性质，例如改变的蛋白水解活性，改变的稳定性等。取代的氨基酸残基对应于与一个或多个以下残基组合的位置+76， +101，+103，+104，+107，+123，+27，+105，+ 109，+126，+128，+135，+ 156，+166，+ 195，+ 197，+ 204，+ 206 ，+210，+ 216，+ 217，+ 218，+ 222，+ 260，+ 265和/或+ 274的解淀粉芽孢杆菌枯草杆菌蛋白酶。
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27.发明授权
公开(公告)号：US6033890A
公开(公告)日：2000-03-07
申请号：US445463
申请日：1995-05-22
申请人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
发明人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
IPC分类号： G01N33/542 , C12N9/04 , C12N15/09 , C12Q1/28 , C12Q1/32 , C12R1/19 , G01N33/535 , G01N33/569 , G01N33/58 , G01N33/78 , G01N33/94
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
摘要翻译： 本发明涉及使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及通过每个亚基的至少一个氨基酸的缺失，取代或插入或其任何组合，使用不同于任何前体G6PDH的分析物或分析物类似物和突变体NAD +依赖性G6PDH的缀合物的用途。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。
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公开(公告)号：US09469848B2
公开(公告)日：2016-10-18
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
IPC分类号： C12P19/20 , C12N9/34 , C07H21/04 , C07K1/00 , C12N9/26 , A23L1/09 , C12C5/00 , C12C7/04 , C12P19/02 , C13K1/06
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08431121B2
公开(公告)日：2013-04-30
申请号：US12953378
申请日：2010-11-23
申请人： Benjamin G. Davis , John Bryan Jones , Richard R. Bott , Karl John Sanford , David Aaron Estell
发明人： Benjamin G. Davis , John Bryan Jones , Richard R. Bott , Karl John Sanford , David Aaron Estell
IPC分类号： A61K38/48
CPC分类号： B82Y5/00 , A61K47/51 , A61K47/665 , A61K47/6898
摘要： This invention provides chimeric molecules that are catalytic antagonists of a target molecule. The catalytic antagonists of this invention preferably comprise a targeting moiety attached to an enzyme that degrades the molecule specifically bound by the targeting moiety. The catalytic antagonists of this invention thus bind to a target recognized by the targeting moiety (e.g., a receptor) the enzyme component of the chimera then degrades all or part of the target. This typically results in a reduction or loss of activity of the target and release of the chimeric molecule. The chimeric molecule is then free to attack and degrade another target molecule.
摘要翻译： 本发明提供作为靶分子的催化拮抗剂的嵌合分子。 本发明的催化拮抗剂优选包含与降解由靶向部分特异性结合的分子的酶连接的靶向部分。 因此，本发明的催化拮抗剂与靶向部分识别的靶（例如受体）结合，嵌合体的酶组分然后降解靶的全部或部分。 这通常导致靶的活性降低或丧失，并释放嵌合分子。 然后嵌合分子可以自由地攻击和降解另一个靶分子。
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公开(公告)号：US20120149087A1
公开(公告)日：2012-06-14
申请号：US13251116
申请日：2011-09-30
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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