公开(公告)号：US20100320086A1

公开(公告)日：2010-12-23

申请号：US12779705

申请日：2010-05-13

申请人： Keonjae LEE ,  Sang Yong LEE ,  Seung Jun KIM ,  Hyeon Kyun YOO ,  Seok Jae LEE ,  Chi Won AHN ,  Jae Hong PARK ,  Tae Jung PARK ,  Sang Yup LEE

发明人： Keonjae LEE ,  Sang Yong LEE ,  Seung Jun KIM ,  Hyeon Kyun YOO ,  Seok Jae LEE ,  Chi Won AHN ,  Jae Hong PARK ,  Tae Jung PARK ,  Sang Yup LEE

IPC分类号： G01N27/26 ,  B05D5/12

CPC分类号： G01N33/5438

摘要： Provided are a flexible biosensor using a gold binding substance and a method for manufacturing the same.The flexible biosensor includes: a flexible substrate; a silicon substrate which is formed on the flexible substrate and on which source and drain regions doped with a first type impurity are formed with a predetermined gap; and source, drain and gate electrodes which are formed on the silicon substrate and comprise gold, wherein, on the gate electrode, a fused protein which is formed by fusion with a gold binding substance specifically binding to gold is immobilized. Since the biosensor is embodied on a flexible substrate, it may effectively overcome the limitation of the existing biosensor embodied on a silicon substrate.

摘要翻译： 提供使用金结合物质的柔性生物传感器及其制造方法。 柔性生物传感器包括：柔性基底; 形成在柔性基板上并在其上以预定间隙形成掺杂有第一类型杂质的源极和漏极区域的硅衬底; 以及形成在硅衬底上并包含金的源极，漏极和栅电极，其中在栅电极上固定通过与特异性结合金的金结合物质的融合而形成的融合蛋白。 由于生物传感器体现在柔性基板上，所以可以有效地克服在硅衬底上体现的现有生物传感器的限制。

公开(公告)号：US20100167951A1

公开(公告)日：2010-07-01

申请号：US12522392

申请日：2007-01-08

申请人： Sang Yup Lee ,  Seung Min Yoo ,  Ki-Chang Keum ,  Nae-Choon Yoo ,  Won-Min Yoo ,  June-Myung Kim ,  Jun Yong Choi

发明人： Sang Yup Lee ,  Seung Min Yoo ,  Ki-Chang Keum ,  Nae-Choon Yoo ,  Won-Min Yoo ,  June-Myung Kim ,  Jun Yong Choi

IPC分类号： C40B40/06 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q1/6837

摘要： The present invention relates to nucleic acid probes specific to Staphylococcus aureus, which is useful for detecting and identifying S. aureus in a biological sample. More particularly, the present invention relates to a DNA chip for detecting and identifying S. aureus, on which nucleic acid probes derived from 23 S rRNA gene of S. aureus are immobilized. The application of the DNA chips according to the present invention allows time-saving and accurate diagnosis of bacterial infection compared with the conventional bacterial culture methods.

摘要翻译： 本发明涉及对金黄色葡萄球菌特异性的核酸探针，其可用于检测和鉴定生物样品中的金黄色葡萄球菌。 更具体地，本发明涉及用于检测和鉴定金黄色葡萄球菌的DNA芯片，其上固定有金黄色葡萄球菌23S rRNA基因的核酸探针。 与常规细菌培养方法相比，根据本发明的DNA芯片的应用允许节省细菌感染的准确诊断。

公开(公告)号：US20100050298A1

公开(公告)日：2010-02-25

申请号：US12312676

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： A01H5/00 ,  C12N9/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12P7/62

CPC分类号： C12N9/1025 ,  C12N15/8243 ,  C12P7/625

摘要： The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated from Pseudomonas sp. 6-19 (KCTC 11027BP) which can prepare lactate polymer and/or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for preparing lactate polymer and/or copolymer with the synthase mutant. The polyhydroxyalkanoate synthase mutants of the present invention originated from Pseudomonas sp. 6-19 can efficiently prepare lactate polymer and/or copolymer by using as a substrate lactyl-CoA which is difficult to be used as a substrate by conventional polyhydroxyalkanoate synthase.

摘要翻译： 本发明涉及源于假单胞菌属的聚羟基链烷酸酯合成酶（PHA合酶）突变体。 6-19（KCTC 11027BP），其可以通过使用乳酰辅酶A作为底物制备乳酸聚合物和/或共聚物。 本发明涉及一种用合成酶突变体制备乳酸聚合物和/或共聚物的方法。 本发明的聚羟基链烷酸酯合酶突变体起源于假单胞菌属 6-19可以通过使用难以用作常规聚羟基链烷酸酯合成酶的底物的底物乳酰基辅酶A来有效地制备乳酸聚合物和/或共聚物。

公开(公告)号：US20090298706A1

公开(公告)日：2009-12-03

申请号：US12159933

申请日：2006-12-29

申请人： Sang Yup Lee ,  Tae Jung Park ,  Nam Su Heo ,  Jong Hyun Choi

发明人： Sang Yup Lee ,  Tae Jung Park ,  Nam Su Heo ,  Jong Hyun Choi

IPC分类号： C40B30/04 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C40B50/14

CPC分类号： C07K14/32 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/70 ,  C12P21/02

摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.

摘要翻译： 本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。 更具体地，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BclA或其片段的bclA基因的表达载体，并且靶蛋白可以以与BclA或BclA融合的形式在细胞表面表达 当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。 根据本发明的表达载体能够使用BclA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达 通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

公开(公告)号：US07491528B2

公开(公告)日：2009-02-17

申请号：US10600145

申请日：2003-06-19

申请人： Sang-Yup Lee ,  Ki-Jun Jeong

发明人： Sang-Yup Lee ,  Ki-Jun Jeong

IPC分类号： C12P21/06 ,  C12P21/04 ,  C12N15/64 ,  C12N1/20 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12P21/02 ,  C07K14/245 ,  C07K14/675 ,  C07K2319/02 ,  C07K2319/035 ,  C07K2319/75 ,  C12N15/625 ,  C12N15/70

摘要： The present invention provides an expression vector comprising genes encoding OmpF of E. coli and a desired protein, E.coli transformed with the expression vector, and a method for extracellular production of desired proteins by employing the same. The recombinant expression vector of the invention comprises an ampicillin-resistance gene, the OmpF promoter and the OmpF gene. In accordance with the invention, a desired protein can be produced extracellularly by a simpler method than conventional methods such that: secretory production of OmpF fusion protein begins simultaneously with growth of the cells through constitutive expression employing an OmpF promoter, and as the concentration of cells increases, the amount of secretory production of the protein also increases continuously. Therefore, desired proteins can be produced in large quantities by a high concentration culture of cells.

摘要翻译： 本发明提供了包含编码大肠杆菌OmpF和所需蛋白质的基因的表达载体，用表达载体转化的大肠杆菌，以及通过使用该方法细胞外产生所需蛋白质的方法。 本发明的重组表达载体包含氨苄青霉素抗性基因，OmpF启动子和OmpF基因。 根据本发明，可以通过比常规方法更简单的方法在细胞外产生所需的蛋白质，使得OmpF融合蛋白的分泌生成与通过使用OmpF启动子的组成型表达的细胞的生长同时开始，并且作为细胞的浓度 增加，蛋白质的分泌生产量也不断增加。 因此，可以通过高浓度细胞培养物大量生产所需的蛋白质。

公开(公告)号：US20080199907A1

公开(公告)日：2008-08-21

申请号：US11915314

申请日：2005-05-23

申请人： Sang-Yup Lee ,  Mee Jung Han

发明人： Sang-Yup Lee ,  Mee Jung Han

IPC分类号： C12P21/04 ,  C07K16/00

CPC分类号： C07K1/14

摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.

摘要翻译： 本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。 根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。 此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sIISP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

公开(公告)号：USD570252S1

公开(公告)日：2008-06-03

申请号：US29251419

申请日：2006-01-06

申请人： Thomas V Peters ,  Sang Yup Lee ,  Steve Kim ,  Vladimir Kapitonov

设计人： Thomas V Peters ,  Sang Yup Lee ,  Steve Kim ,  Vladimir Kapitonov

公开(公告)号：US20070065817A1

公开(公告)日：2007-03-22

申请号：US10514072

申请日：2003-05-09

申请人： Sang-Yup Lee ,  Seung Yoo ,  Kyung Chang ,  So Yoo ,  Nae Yo ,  Won Yoo ,  Ki Keum ,  June Kim ,  Gene Lee

发明人： Sang-Yup Lee ,  Seung Yoo ,  Kyung Chang ,  So Yoo ,  Nae Yo ,  Won Yoo ,  Ki Keum ,  June Kim ,  Gene Lee

IPC分类号： C12Q1/68 ,  C12M3/00 ,  C12M1/34 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： The present invention relates to nucleic acid probes which are derived from rRNA genes of non-virus organisms and are useful for the detection of said non-virus infectious organisms in a biological sample. In addition, the present invention relates to compositions and chips useful for the diagnosis of one or more types of infectious diseases comprising said nucleic acid probes.

摘要翻译： 本发明涉及衍生自非病毒生物体的rRNA基因的核酸探针，并且可用于检测生物样品中的所述非病毒感染性生物体。 此外，本发明涉及可用于诊断包含所述核酸探针的一种或多种感染性疾病的组合物和碎片。

公开(公告)号：US20060213790A1

公开(公告)日：2006-09-28

申请号：US10552335

申请日：2004-04-03

申请人： Sang-Yup Lee

发明人： Sang-Yup Lee

IPC分类号： B65D85/18

CPC分类号： A47G25/54 ,  A45C3/004 ,  A45C3/04 ,  A45C9/00 ,  A47G25/32 ,  B65D85/185

摘要： The present invention relates to a shopping bag that can be used as a coat hanger and a packing supplies for a gift. According to the present invention, a shopping bag for clothes is reused as a coat hanger. Also even when the clothes should be kept for a long term due to change of season, the clothes can be kept clean without dirt. The shopping according to the present invention is simply constructed with single piece of paper and single coat hanger so that it does not need any other packing materials such as scissors, a tape, a ruler, a packing paper, a shopping bag, and the like.

摘要翻译： 本发明涉及一种可用作衣架的购物袋和用于礼物的包装用品。 根据本发明，用于衣服的购物袋被重新用作衣架。 即使衣服由于季节变化而长期保存，衣服也可以保持清洁无污染。 根据本发明的购物单纯地由单张纸和单衣架构成，从而不需要任何其它包装材料，例如剪刀，胶带，尺子，包装纸，购物袋等 。

公开(公告)号：US06989251B2

公开(公告)日：2006-01-24

申请号：US10300711

申请日：2002-11-19

申请人： Sang-Yup Lee ,  Ho-Nam Chang ,  Nagendra Narayan Thakur ,  Jin-Hwan Do

发明人： Sang-Yup Lee ,  Ho-Nam Chang ,  Nagendra Narayan Thakur ,  Jin-Hwan Do

IPC分类号： C12P21/04 ,  C12P13/14

CPC分类号： C12P21/02

摘要： The present invention provides a method for manufacturing highly-concentrated polyglutamic acid by culturing an aerobic micro-organism of Bascillus sp. with the additional supply of saccharides. The method for manufacturing polyglutamic acid comprises a step of culturing Bascillus sp. in a fed-batch or batch culture while supplying saccharides to the culture. Since the method for manufacturing highly-concentrated polyglutamic acid can be applicable to the industrial scale fermentation, mass production of polyglutamic acid can be feasible in a cost-efficient way.