公开(公告)号：US20230384313A1

公开(公告)日：2023-11-30

申请号：US18322442

申请日：2023-05-23

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Joshua LaBaer ,  Ji Qiu ,  Lusheng Song ,  Yunro Chung

IPC分类号： G01N33/574

CPC分类号： G01N33/57446 ,  G01N2333/205 ,  G01N2800/52 ,  G01N2800/50

摘要： Provided are methods and compositions for identifying individuals having intestinal metaplasia (IM) and/or Helicobacter pylori infection based on the levels of antibodies against particular Helicobacter pylori antigens. Patient diagnosis allows for medical interventions, including therapeutic treatments, disease progression preventative measures and enhanced cancer screening. Also provided are methods and compositions for identifying individuals having IM who are at increased risk for developing gastric cancer. Patient diagnosis and stratification by risk allows for targeted screening and monitoring for signs of gastric cancer as well as earlier intervention with cancer preventative treatments.

公开(公告)号：US20220163531A1

公开(公告)日：2022-05-26

申请号：US17563279

申请日：2021-12-28

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Benjamin Katchman ,  Karen Anderson ,  Garrick Wallstrom ,  Joshua LaBaer ,  Daniel Cramer

IPC分类号： G01N33/574

摘要： Compositions and methods relating to a panel of antigen biomarkers for the early detection of ovarian cancer. The compositions and methods encompass antigen biomarkers coupled to a substrate, with the biomarkers being selected from the group consisting of one or more of ICAM3, CTAG2, p53, STYXL1, PVR, POMC, NUDT11, TRIM39, UHMK1, KSR1, and NXF3.

公开(公告)号：US10787710B2

公开(公告)日：2020-09-29

申请号：US16532138

申请日：2019-08-05

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Joshua LaBaer ,  Kristin Gillis ,  Garrick Wallstrom ,  Jin Park ,  Vel Murugan ,  Mitch Magee

IPC分类号： C12Q1/6883 ,  C12Q1/6827

摘要： Disclosed herein are compositions and methods for accurately estimating the absorbed dose of radiation indicated by a subject based on the expression pattern of a panel of radiation-modulated (RM) genes at various time points following exposure of the subject to ionizing radiation.

公开(公告)号：US20170088830A1

公开(公告)日：2017-03-30

申请号：US15310868

申请日：2015-05-14

申请人： Arizona Board of Regents on behalf of Arizona State University

发明人： Joshua LaBaer

IPC分类号： C12N11/06 ,  C12N9/10 ,  C12P19/02 ,  C12N9/14 ,  C12P19/14 ,  C12N15/10 ,  C12N9/42

CPC分类号： C12N11/06 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1029 ,  C12N9/14 ,  C12N9/2437 ,  C12N9/96 ,  C12N15/10 ,  C12N15/1068 ,  C12P19/02 ,  C12P19/14 ,  C12Y201/01063 ,  C12Y203/01 ,  C12Y302/01004 ,  C12Y308/01005

摘要： Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.

公开(公告)号：US20240229132A1

公开(公告)日：2024-07-11

申请号：US18480850

申请日：2023-10-04

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Jia Guo ,  Joshua LaBaer

IPC分类号： C12Q1/6874 ,  C12Q1/6876

CPC分类号： C12Q1/6874 ,  C12Q1/6876 ,  C12Q2563/131

摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable fluorescent streptavidin. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US11814677B2

公开(公告)日：2023-11-14

申请号：US17138287

申请日：2020-12-30

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Jia Guo ,  Joshua LaBaer

IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6876

CPC分类号： C12Q1/6874 ,  C12Q1/6876 ,  C12Q2563/131

摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US20220373548A1

公开(公告)日：2022-11-24

申请号：US17771595

申请日：2020-10-23

申请人： Arizona Board of Regents on behalf of Arizona State University

发明人： Joshua LaBaer ,  Lusheng Song ,  Ji Qiu ,  Yunro Chung

IPC分类号： G01N33/569 ,  G01N33/574 ,  A61P35/00

摘要： Provided herein are methods, compositions, kits, and systems for detecting Epstein Barr virus infection (EBV) in gastric cancer (GC) patients. In particular, provided herein are methods, composition, kits, and systems for diagnosing and treating EBV-positive gastric cancer (EBV+ GC) in a biological sample of an individual based on the presence and level of antibodies against particular Epstein Barr virus proteins. EBV+ GC is a distinct subtype of gastric cancer and is associated with unique molecular profiles. Also provided herein are methods for providing more personalized therapy for each of these distinct cancer subtypes and methods for determining an Epstein Barr virus-positive gastric cancer antibody signature, and kits comprising components and protocols for performing the methods of this disclosure.

公开(公告)号：US11060133B2

公开(公告)日：2021-07-13

申请号：US16172379

申请日：2018-10-26

申请人： Arizona Board of Regents on Behalf of Arizona State University ,  Mayo Foundation for Medical Education and Research

发明人： Joshua LaBaer ,  Vel Murugan ,  Merica Vorachitti ,  Rahul Pannala ,  Douglas Faigel

IPC分类号： C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/689

摘要： Provided herein are qPCR-based methods for quantitatively detecting and differentiating between live and dead pathogenic bacteria such as carbapenem-resistant Enterobacteriaceae (CRE). Also provided herein are simple, fast, and reliable genomic DNA extraction methods with improved efficiency.

公开(公告)号：US20210198735A1

公开(公告)日：2021-07-01

申请号：US17138287

申请日：2020-12-30

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Jia Guo ,  Joshua LaBaer

IPC分类号： C12Q1/6874 ,  C12Q1/6876

摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US10717977B2

公开(公告)日：2020-07-21

申请号：US15904905

申请日：2018-02-26

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Joshua LaBaer

IPC分类号： C12N15/10 ,  G01N33/68 ,  G01N33/53

摘要： Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.