公开(公告)号：US20120074066A1

公开(公告)日：2012-03-29

申请号：US12889108

申请日：2010-09-23

申请人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： G01N27/416 ,  B01D15/08 ,  G01N30/02 ,  B01D17/06

CPC分类号： G01N27/44743 ,  G01N27/44791 ,  G01N30/461

摘要： Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.

摘要翻译： 使用基于机械阀而不是电动注射的样品注射器的微芯片毛细管电泳（CE）可以提供改进的样品注射，增强的能力，并且可以消除改变分离通道中的电场以诱导注射样品的需要。 在一个实例中，CE电极沿着分离通道连续地施加用于CE分离的电场。 样品通道在交叉口处连接到分离通道，并且具有大于在交叉口附近的分离通道中存在的样品压力的样品压力。 样品通道不具有施加电动注射电压的电极。 样品通道或交叉口处的样品注射器包括一个机械阀，用于控制从样品通道到分离通道的样品注射。

公开(公告)号：US20100269573A1

公开(公告)日：2010-10-28

申请号：US12430490

申请日：2009-04-27

申请人： Ryan T. Kelly ,  Keqi Tang ,  Jason S. Page ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Keqi Tang ,  Jason S. Page ,  Richard D. Smith

IPC分类号： G01N30/84 ,  H01J49/00

CPC分类号： B01L3/502784 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44743 ,  G01N30/7266 ,  H01J49/00

摘要： A transfer structure for droplet-based microfluidic analysis is characterized by a first conduit containing a first stream having at least one immiscible droplet of aqueous material and a second conduit containing a second stream comprising an aqueous fluid. The interface between the first conduit and the second conduit can define a plurality of apertures, wherein the apertures are sized to prevent exchange of the first and second streams between conduits while allowing lossless transfer of droplets from the first conduit to the second conduit through contact between the first and second streams.

摘要翻译： 用于基于液滴的微流体分析的转移结构的特征在于包含具有至少一种不混溶的水性材料的液滴的第一流的第一导管和含有含水流体的第二流的第二导管。 第一导管和第二导管之间的界面可以限定多个孔，其中孔的尺寸设定成防止第一和第二流在导管之间的交换，同时允许液滴从第一导管到第二导管的无损转移， 第一和第二个流。

公开(公告)号：US20090230296A1

公开(公告)日：2009-09-17

申请号：US12046207

申请日：2008-03-11

申请人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： B01D59/44

CPC分类号： H01J49/167

摘要： Electrospray ionization emitter arrays, as well as methods for forming electrosprays, are described. The arrays are characterized by a radial configuration of three or more nano-electrospray ionization emitters without an extractor electrode. The methods are characterized by distributing fluid flow of the liquid sample among three or more nano-electrospray ionization emitters, forming an electrospray at outlets of the emitters without utilizing an extractor electrode, and directing the electrosprays into an entrance to a mass spectrometry device. Each of the nano-electrospray ionization emitters can have a discrete channel for fluid flow. The nano-electrospray ionization emitters are circularly arranged such that each is shielded substantially equally from an electrospray-inducing electric field.

摘要翻译： 描述了电喷雾电离发射器阵列，以及用于形成电喷雾的方法。 阵列的特征在于没有提取器电极的三个或更多个纳米电喷雾电离发射器的径向构型。 该方法的特征在于在三个或更多个纳米电喷雾电离发射器之间分配液体样品的流体流动，在不使用提取器电极的情况下在发射器的出口处形成电喷雾，并将电喷雾引导到质谱装置的入口。 每个纳米电喷雾电离发射器可以具有用于流体流动的离散通道。 纳米电喷雾电离发射器是圆形布置的，使得每个发射器基本上等同于电喷雾诱导电场屏蔽。

公开(公告)号：US08907273B1

公开(公告)日：2014-12-09

申请号：US14292437

申请日：2014-05-30

申请人： Tsung-Chi Chen ,  Keqi Tang ,  Yehia M. Ibrahim ,  Richard D. Smith ,  Gordon A. Anderson ,  Erin M. Baker

发明人： Tsung-Chi Chen ,  Keqi Tang ,  Yehia M. Ibrahim ,  Richard D. Smith ,  Gordon A. Anderson ,  Erin M. Baker

IPC分类号： H01J49/06

CPC分类号： H01J49/062 ,  G01N27/622 ,  H01J49/06

摘要： An ion manipulation method and device is disclosed. The device includes a pair of substantially parallel surfaces. An array of inner electrodes is contained within, and extends substantially along the length of, each parallel surface. The device includes a first outer array of electrodes and a second outer array of electrodes. Each outer array of electrodes is positioned on either side of the inner electrodes, and is contained within and extends substantially along the length of each parallel surface. A DC voltage is applied to the first and second outer array of electrodes. A RF voltage, with a superimposed electric field, is applied to the inner electrodes by applying the DC voltages to each electrode. Ions either move between the parallel surfaces within an ion confinement area or along paths in the direction of the electric field, or can be trapped in the ion confinement area. A predetermined number of pairs of surfaces are disposed in one or more chambers, forming a multiple-layer ion mobility cyclotron device.

摘要翻译： 公开了一种离子操作方法和装置。 该装置包括一对基本平行的表面。 内部电极的阵列包含在每个平行表面内并且基本上沿着每个平行表面的长度延伸。 该装置包括电极的第一外部阵列和第二外部电极阵列。 每个外部电极阵列位于内部电极的任一侧上，并且基本上沿着每个平行表面的长度容纳并延伸。 DC电压施加到第一和第二外部电极阵列。 通过向每个电极施加直流电压，将具有叠加电场的RF电压施加到内部电极。 离子在离子限制区域内的平行表面之间或沿着电场方向的路径移动，或者可以被捕获在离子限制区域中。 预定数量的一对表面设置在一个或多个室中，形成多层离子迁移回旋加速器装置。

公开(公告)号：US20120318672A1

公开(公告)日：2012-12-20

申请号：US13596360

申请日：2012-08-28

申请人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： G01N27/447

CPC分类号： G01N27/44743 ,  G01N27/44791 ,  G01N30/461

摘要： Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.

摘要翻译： 使用基于机械阀而不是电动注射的样品注射器的微芯片毛细管电泳（CE）可以提供改进的样品注射，增强的能力，并且可以消除改变分离通道中的电场以诱导注射样品的需要。 在一个实例中，CE电极沿着分离通道连续地施加用于CE分离的电场。 样品通道在交叉口处连接到分离通道，并且具有大于在交叉口附近的分离通道中存在的样品压力的样品压力。 样品通道不具有施加电动注射电压的电极。 样品通道或交叉口处的样品注射器包括一个机械阀，用于控制从样品通道到分离通道的样品注射。

公开(公告)号：US08277659B2

公开(公告)日：2012-10-02

申请号：US12889108

申请日：2010-09-23

申请人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： B01D15/08

CPC分类号： G01N27/44743 ,  G01N27/44791 ,  G01N30/461

摘要： Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.

摘要翻译： 使用基于机械阀而不是电动注射的样品注射器的微芯片毛细管电泳（CE）可以提供改进的样品注射，增强的能力，并且可以消除改变分离通道中的电场以诱导注射样品的需要。 在一个实例中，CE电极沿着分离通道连续地施加用于CE分离的电场。 样品通道在交叉口处连接到分离通道，并且样品压力大于在交叉口附近的分离通道中存在的样品压力。 样品通道不具有施加电动注射电压的电极。 样品通道或交叉口处的样品注射器包括一个机械阀，用于控制从样品通道到分离通道的样品注射。

公开(公告)号：US07816645B2

公开(公告)日：2010-10-19

申请号：US12046207

申请日：2008-03-11

申请人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： H01J49/10

CPC分类号： H01J49/167

摘要： Electrospray ionization emitter arrays, as well as methods for forming electrosprays, are described. The arrays are characterized by a radial configuration of three or more nano-electrospray ionization emitters without an extractor electrode. The methods are characterized by distributing fluid flow of the liquid sample among three or more nano-electrospray ionization emitters, forming an electrospray at outlets of the emitters without utilizing an extractor electrode, and directing the electrosprays into an entrance to a mass spectrometry device. Each of the nano-electrospray ionization emitters can have a discrete channel for fluid flow. The nano-electrospray ionization emitters are circularly arranged such that each is shielded substantially equally from an electrospray-inducing electric field.

摘要翻译： 描述了电喷雾电离发射器阵列，以及用于形成电喷雾的方法。 阵列的特征在于没有提取器电极的三个或更多个纳米电喷雾电离发射器的径向构型。 该方法的特征在于在三个或更多个纳米电喷雾电离发射器之间分配液体样品的流体流动，在不使用提取器电极的情况下在发射器的出口处形成电喷雾，并将电喷雾引导到质谱装置的入口。 每个纳米电喷雾电离发射器可以具有用于流体流动的离散通道。 纳米电喷雾电离发射器是圆形布置的，使得每个发射器基本上等同于电喷雾诱导电场屏蔽。

公开(公告)号：US20090057551A1

公开(公告)日：2009-03-05

申请号：US11848884

申请日：2007-08-31

申请人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

发明人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

IPC分类号： H01J49/04 ,  B05B5/025

CPC分类号： H01J49/165 ,  H01J49/066

摘要： A system and method are disclosed that provide up to complete transmission of ions between coupled stages with low effective ion losses. A novel “interfaceless” electrospray ionization system is further described that operates the electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer.

摘要翻译： 公开了一种系统和方法，其提供在具有低有效离子损耗的耦合级之间的离子的完全传输。 进一步描述了一种新颖的“无接触”电喷雾离子化系统，其在减压下操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦并将离子传输到质量分析器中的电动离子漏斗中。

公开(公告)号：US06979816B2

公开(公告)日：2005-12-27

申请号：US10400356

申请日：2003-03-25

申请人： Keqi Tang ,  Mikhail B. Belov ,  Aleksey V. Tolmachev ,  Harold R. Udseth ,  Richard D. Smith

发明人： Keqi Tang ,  Mikhail B. Belov ,  Aleksey V. Tolmachev ,  Harold R. Udseth ,  Richard D. Smith

IPC分类号： H01J49/06 ,  H01J49/42 ,  H01J49/04

CPC分类号： H01J49/066 ,  H01J49/107 ,  H01J49/165

摘要： A method for introducing ions generated in a region of relatively high pressure into a region of relatively low pressure by providing at least two electrospray ion sources, providing at least two capillary inlets configured to direct ions generated by the electrospray sources into and through each of the capillary inlets, providing at least two sets of primary elements having apertures, each set of elements having a receiving end and an emitting end, the primary sets of elements configured to receive a ions from the capillary inlets at the receiving ends, and providing a secondary set of elements having apertures having a receiving end and an emitting end, the secondary set of elements configured to receive said ions from the emitting end of the primary sets of elements and emit said ions from said emitting end of the secondary set of elements. The method may further include the step of providing at least one jet disturber positioned within at least one of the sets of primary elements, providing a voltage, such as a dc voltage, in the jet disturber, thereby adjusting the transmission of ions through at least one of the sets of primary elements.

摘要翻译： 一种通过提供至少两个电喷雾离子源将相对较高压力区域产生的离子引入至少两个电喷雾离子源的方法，提供至少两个毛细管入口，其构造成将由电喷雾源产生的离子引入和穿过每个 毛细管入口，提供具有孔的至少两组主要元件，每组元件具有接收端和发射端，所述主要元件组被配置为在接收端处从毛细管入口接收离子，并且提供次级 具有孔的一组元件具有接收端和发射端，所述次级元件被配置为从所述主要元件组的发射端接收所述离子，并从所述次级元件组的所述发射端发射所述离子。 该方法还可以包括提供位于主要元件组中的至少一个中的至少一个喷射干燥器的步骤，在喷射干燥器中提供诸如直流电压的电压，从而通过至少调节离子的透过率 其中一个主要元素。

公开(公告)号：US06831274B2

公开(公告)日：2004-12-14

申请号：US10091833

申请日：2002-03-05

申请人： Richard D. Smith ,  Keqi Tang ,  Yuehe Lin

发明人： Richard D. Smith ,  Keqi Tang ,  Yuehe Lin

IPC分类号： B01D5944

CPC分类号： H01J49/165 ,  H01J49/0018 ,  H01J49/107

摘要： A method and apparatus that utilizes two or more emitters simultaneously to form an electrospray of a sample that is then directed into a mass spectrometer, thereby increasing the total ion current introduced into an electrospray ionization mass spectrometer, given a liquid flow rate of a sample. The method and apparatus are most conveniently constructed as an array of spray emitters fabricated on a single chip, however, the present invention encompasses any apparatus wherein two or more emitters are simultaneously utilized to form an electrospray of a sample that is then directed into a mass spectrometer.

摘要翻译： 同时使用两个或更多个发射体形成样品的电喷雾的方法和装置，该样品的电喷雾随后被引导到质谱仪中，从而在给定样品的液体流速的情况下增加引入电喷雾离子化质谱仪中的总离子电流。 该方法和装置最方便地构造为在单个芯片上制造的喷射发射体的阵列，然而，本发明包括其中两个或更多个发射体同时用于形成样品的电喷雾的任何装置，然后将其引导到质量 光谱仪