Chip, detection system and gene sequencing method

    公开(公告)号:US11168361B2

    公开(公告)日:2021-11-09

    申请号:US16072923

    申请日:2017-12-15

    IPC分类号: C12Q1/6869 C12Q1/6837

    摘要: A chip, a detection system and a gene sequencing method are provided. When the chip is used for gene sequencing, sample genes and reversible terminating nucleotides are added into micropores and matched therein to release hydrogen ions such that a Nernst potential is induced on an ion-sensitive film surface, and a voltage is applied to the transparent electrode layer to generate an electric field, thereby controlling the switching layer to change its state, and then a base type of the genes is determined based on a type of reversible terminating nucleotide corresponding to information of light emitted from the switching layer upon changes in the state of the switching layer, thereby gene sequencing is achieved.