公开(公告)号：US20090201501A1

公开(公告)日：2009-08-13

申请号：US12197192

申请日：2008-08-22

申请人： Nathaniel C. Bair ,  Collin A. Rich ,  Richard L. Fisher

发明人： Nathaniel C. Bair ,  Collin A. Rich ,  Richard L. Fisher

IPC分类号： G01N21/53

CPC分类号： G01N21/53 ,  G01N15/1434 ,  G01N2021/6497 ,  Y10T436/115831

摘要： The preferred embodiments of the invention is an optical system for a flow cytometer including a flow channel with an interrogation zone, and an illumination source that impinges the flow channel in the interrogation zone from a particular direction. The optical system preferably includes a lens system and a detection system. The lens system preferably includes multiple lens surfaces arranged around the flow channel and adapted to collect and collimate light from the interrogation zone. The detection system preferably includes multiple detectors adapted to detect light from the lens system. Each detector preferably includes a local filter that independently filters for specific wavelengths. Thus, the user may easily swap the filters in any order to achieve the same detection parameters.

摘要翻译： 本发明的优选实施例是用于流式细胞仪的光学系统，其包括具有询问区的流动通道，以及照射源，其将特定方向上的询问区中的流动通道入射。 光学系统优选地包括透镜系统和检测系统。 透镜系统优选地包括围绕流动通道设置的多个透镜表面，并且适于收集和准直来自询问区域的光。 检测系统优选地包括适于检测来自透镜系统的光的多个检测器。 每个检测器优选地包括独立地滤波特定波长的局部滤波器。 因此，用户可以以任何顺序容易地交换滤波器以实现相同的检测参数。

公开(公告)号：US06926670B2

公开(公告)日：2005-08-09

申请号：US10054330

申请日：2002-01-22

申请人： Collin A. Rich ,  Yafan Zhang ,  Nader Najafi ,  Matthew Z. Straayer ,  Sonbol Massoud-Ansari

发明人： Collin A. Rich ,  Yafan Zhang ,  Nader Najafi ,  Matthew Z. Straayer ,  Sonbol Massoud-Ansari

IPC分类号： A61B5/00 ,  A61B5/0215 ,  A61B5/027 ,  A61B8/14

CPC分类号： A61B5/6852 ,  A61B5/0031 ,  A61B5/02028 ,  A61B5/0215 ,  A61B5/027 ,  A61B5/0295 ,  A61B5/14532 ,  A61B5/14539 ,  A61B5/14546 ,  A61B5/1473 ,  A61B5/4839 ,  A61B5/6862 ,  A61B5/6876 ,  A61B5/6882 ,  A61B2562/028

摘要： The present invention relates to an implantable microfabricated sensor device and system for measuring a physiologic parameter of interest within a patient. The implantable device is micro electromechanical system (MEMS) device and includes a substrate having an integrated inductor and at least one sensor formed thereon. A plurality of conductive paths electrically connect the integrated inductor with the sensor. Cooperatively, the integrated inductor, sensor and conductive paths defining an LC tank resonator.

摘要翻译： 本发明涉及一种用于测量患者内感兴趣的生理参数的可植入微制造传感器装置和系统。 可植入装置是微机电系统（MEMS）装置，并且包括具有集成电感器的基板和形成在其上的至少一个传感器。 多个导电路径将集成电感器与传感器电连接。 合成的集成电感器，传感器和导电路径限定了LC槽谐振器。

公开(公告)号：US08507279B2

公开(公告)日：2013-08-13

申请号：US12792536

申请日：2010-06-02

申请人： Jack T. Ball ,  Grant C. Howes ,  Collin A. Rich

发明人： Jack T. Ball ,  Grant C. Howes ,  Collin A. Rich

IPC分类号： G01N31/00 ,  G01N33/00 ,  G01N21/01 ,  G01N21/00

CPC分类号： G01N33/5094 ,  G01N15/1012 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1459 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1018 ,  G01N2015/1486 ,  G01N2333/70514 ,  Y10T436/10 ,  Y10T436/101666

摘要： A system and method for a flow cytometer system including a prepared sample fluid with reference beads; an interrogation zone that analyzes the prepared sample fluid; a peristaltic pump system that draws the sample fluid through the interrogation zone; and a processor that monitors a measured volume of sample fluid sampled by the peristaltic pump system and an expected sample volume based on data generated by the analysis of the sample fluid. A system and method is additionally described using an alternative volume sensing fluidic system.

摘要翻译： 一种用于流式细胞仪系统的系统和方法，其包括具有参比珠的制备的样品流体; 一个询问区，分析制备的样品液; 蠕动泵系统，通过询问区抽取样品液; 以及处理器，其基于通过对样品流体的分析产生的数据监测由蠕动泵系统采样的样品流体的测量体积和预期样品体积。 使用替代的体积感测流体系统另外描述系统和方法。

公开(公告)号：US08399278B2

公开(公告)日：2013-03-19

申请号：US12974349

申请日：2010-12-21

申请人： David F. Lemmerhirt ,  Collin A. Rich

发明人： David F. Lemmerhirt ,  Collin A. Rich

IPC分类号： H01L21/00

CPC分类号： B06B1/0292 ,  A61B8/00 ,  A61B8/4472

摘要： The integrated circuit/transducer device of the preferred embodiment includes a substrate, a complementary-metal-oxide-semiconductor (CMOS) circuit that is fabricated on the substrate, and a capacitive micromachined ultrasonic transducer (cMUT) element that is also fabricated on the substrate. The CMOS circuit and cMUT element are fabricated during the same foundry process and are connected. The cMUT includes a lower electrode, an upper electrode, a membrane structure that support the upper electrode, and a cavity between the upper electrode and lower electrode.

摘要翻译： 优选实施例的集成电路/换能器装置包括衬底，制造在衬底上的互补金属氧化物半导体（CMOS）电路，以及还制造在衬底上的电容微加工超声换能器（cMUT）元件 。 CMOS电路和cMUT元件在相同的铸造过程中制造并连接。 cMUT包括下电极，上电极，支撑上电极的膜结构，以及上电极和下电极之间的空腔。

公开(公告)号：US08229707B2

公开(公告)日：2012-07-24

申请号：US13175926

申请日：2011-07-04

申请人： David Olson ,  Collin A. Rich ,  Clement James Goebel, III

发明人： David Olson ,  Collin A. Rich ,  Clement James Goebel, III

IPC分类号： G06F17/40 ,  G06F17/00

CPC分类号： G01N15/1429 ,  G01N35/00712 ,  G01N35/0092

摘要： A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.

摘要翻译： 从优选实施例的流式细胞仪系统提取和分析数据集的方法包括以下步骤：（1）运行样本并保存所有收集的原始数据，（2）查看原始（或“未修改”）数据，（3 ）修改原始数据（例如，缩放和/或剔除原始数据），（4）查看和保存修改的数据，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改的数据，以及根据需要和/或期望导出保存的数据的步骤。

公开(公告)号：US08187888B2

公开(公告)日：2012-05-29

申请号：US13178776

申请日：2011-07-08

申请人： Collin A. Rich

发明人： Collin A. Rich

IPC分类号： G01N35/08 ,  G01N33/48

CPC分类号： G01N15/1404 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T436/25375

摘要： The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.

摘要翻译： 优选实施例的流体系统包括护套泵，以将护套流体从护套容器泵送到询问区域，以及废物泵将废液从询问区泵送到废物容器中。 护套泵和/或废水泵将样品流体从样品容器中抽入询问区。 流体系统还包括控制器，用于调节样品流体从样品容器进入询问区域的流速。 流体系统优选地结合到具有包括询问区的流动池的流式细胞仪中。

公开(公告)号：US20110204259A1

公开(公告)日：2011-08-25

申请号：US13033299

申请日：2011-02-23

申请人： Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

发明人： Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

IPC分类号： G01J1/58

CPC分类号： G01N15/1429 ,  G01N15/1459

摘要： A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.

摘要翻译： 一种用于在流式细胞仪中检测荧光染料的方法，包括：接收包含每个标记有第一荧光染料和第二荧光染料中的至少一种的颗粒的样品，其中第一和第二荧光染料具有不同的溢出系数; 检测颗粒，包括用第一检测器和第二检测器检测第一和第二荧光染料; 基于第一和第二荧光色素的检测形成检测到的颗粒的数据集; 表征来自数据集的每个检测到的荧光染料的检测到的溢出系数; 并且基于检测到的溢出系数将检测到的颗粒分选成预测的荧光染色体群体。 一种用于在流式细胞仪中检测荧光染料的系统，包括流动池，固定增益检测系统和处理器，其产生每个检测到的颗粒的检测到的溢出系数，并且基于检测到的溢出系数将检测到的颗粒分解成预测的荧光染色体群。

公开(公告)号：US07996188B2

公开(公告)日：2011-08-09

申请号：US11466391

申请日：2006-08-22

申请人： David Olson ,  Collin A. Rich ,  Clement James Goebel, III

发明人： David Olson ,  Collin A. Rich ,  Clement James Goebel, III

IPC分类号： G01N15/00 ,  G06F17/40

CPC分类号： G01N15/1429 ,  G01N35/00712 ,  G01N35/0092

摘要： A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.

摘要翻译： 从优选实施例的流式细胞仪系统提取和分析数据集的方法包括以下步骤：（1）运行样本并保存所有收集的原始数据，（2）查看原始（或“未修改”）数据，（3 ）修改原始数据（例如，缩放和/或剔除原始数据），（4）查看和保存修改的数据，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改的数据，以及根据需要和/或期望导出保存的数据的步骤。

公开(公告)号：US20110058168A1

公开(公告)日：2011-03-10

申请号：US12887392

申请日：2010-09-21

申请人： Collin A. Rich ,  Nathaniel C. Bair

发明人： Collin A. Rich ,  Nathaniel C. Bair

IPC分类号： G01N21/17

CPC分类号： G01N21/53 ,  G01N15/1434 ,  G01N21/532 ,  G01N21/645 ,  G01N2021/4707 ,  G01N2021/4711 ,  G01N2021/4726 ,  G01N2021/6497 ,  Y10T436/12

摘要： An optical system for a flow cytometer having a flow channel with an interrogation zone and an illumination source that impinges the flow channel in the interrogation zone includes a lens system and a detection system. The lens system preferably includes at least two lens surfaces located on opposite sides of the flow channel and configured to collect and collimate light from the interrogation zone. The detection system, configured to detect light from the lens system, preferably includes first and second detectors, a first filter that passes a first wavelength of light and reflects a second wavelength of light, and a second filter that reflects the first wavelength of light and passes the second wavelength of light, wherein the first and second filters are aligned such that light reflected from the first filter passes into the second detector and light reflected from the second filter passes into the first detector.

摘要翻译： 具有流动通道的流式细胞仪的光学系统具有询问区的流动通道和将询问区中的流动通道照射的照明源包括透镜系统和检测系统。 透镜系统优选地包括位于流动通道的相对侧上的至少两个透镜表面，并且被配置为收集和准直来自询问区的光。 被配置为检测来自透镜系统的光的检测系统优选地包括第一和第二检测器，通过第一波长的光并反射第二波长的光的第一滤光器和反射第一波长的光的第二滤光器，以及 通过第二波长的光，其中第一和第二滤光器对齐，使得从第一滤光片反射的光线进入第二检测器，并且从第二滤光器反射的光线进入第一检测器。

公开(公告)号：US20090260701A1

公开(公告)日：2009-10-22

申请号：US12425830

申请日：2009-04-17

申请人： Collin A. Rich ,  Nathaniel C. Bair

发明人： Collin A. Rich ,  Nathaniel C. Bair

IPC分类号： F16L55/04

CPC分类号： F16L55/04 ,  F04B43/12 ,  F15B1/021 ,  F15B1/10 ,  F15B21/008 ,  F15B2201/205 ,  F15B2201/215 ,  F15B2201/3151 ,  G01N15/1404 ,  G01N15/1459 ,  Y10T137/3118

摘要： A pulsation attenuator for a fluidic system with a fluidic pump. The pulsation attenuator includes a fluidic channel, a first fluidic device adapted to attenuate pulsations, and a second fluidic device adapted to attenuate pulsations. Preferably, the first fluidic device includes a first fluidic resistor and a first fluidic capacitor, and the second fluidic device includes a second fluidic resistor and a second fluidic capacitor. Preferably, the first fluidic resistor and second fluidic resistor are resistive channels. Preferably, the first fluidic capacitor and second fluidic capacitor include a membrane that expands and accumulates fluid and then contracts and reintroduces the accumulated fluid into the fluidic channel.

摘要翻译： 一种具有流体泵的流体系统的脉动衰减器。 脉动衰减器包括流体通道，适于衰减脉动的第一流体装置，以及适于衰减脉动的第二流体装置。 优选地，第一流体装置包括第一流体电阻器和第一流体电容器，并且第二流体装置包括第二流体电阻器和第二流体电容器。 优选地，第一流体电阻器和第二流体电阻器是电阻通道。 优选地，第一流体电容器和第二流体电容器包括膨胀和积聚流体然后收缩并将积聚的流体再引入流体通道的膜。