公开(公告)号：US20150148513A1

公开(公告)日：2015-05-28

申请号：US14405390

申请日：2013-06-03

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12N1/20 ,  C12P7/18 ,  C07C59/01 ,  C07C31/20 ,  C08G69/02 ,  C08G18/28 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C12P7/42 ,  C08G18/32

CPC classification number: C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.

Abstract translation: 本发明提供具有4-羟基丁酸酯，1,4-丁二醇或其它产物途径的非天然存在的微生物生物，并能够生产4-羟基丁酸酯，1,4-丁二醇或其它产品，其中微生物有机体包含一个 或更多遗传修饰。 本发明另外提供了使用微生物生物制备4-羟基丁酸酯，1,4-丁二醇或其它产品或相关产品的方法。

公开(公告)号：US20150080547A1

公开(公告)日：2015-03-19

申请号：US14373160

申请日：2013-01-18

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk

IPC: C12P7/44 ,  C12P7/62 ,  C08G63/183 ,  C12P7/40

CPC classification number: C12P7/44 ,  C07C51/43 ,  C07F9/091 ,  C08G63/183 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/88 ,  C12P7/24 ,  C12P7/40 ,  C12P7/62 ,  C12P9/00 ,  C07C63/26 ,  C07C63/04

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy) phosphonate (2H3M40P) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M40P, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (C02) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

Abstract translation: 本发明提供具有（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯（2H3M40P）途径，对甲苯甲酸途径和/或对苯二酸酯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产2H3M40P，对甲苯甲酸酯或对苯二甲酸酯的方法。 本文还提供了从培养基中分离生物基芳族羧酸，特别是对甲苯甲酸或对苯二甲酸的方法，其中所述方法包括使培养基与足够的二氧化碳（CO 2）接触以降低 该培养基产生由芳族羧酸组成的沉淀物。

公开(公告)号：US20140155567A1

公开(公告)日：2014-06-05

申请号：US14059131

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P5/02 ,  C08F136/06 ,  C07C11/16

CPC classification number: C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

Abstract translation: 本发明提供具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产丁二烯的方法。

公开(公告)号：US11834690B2

公开(公告)日：2023-12-05

申请号：US17527902

申请日：2021-11-16

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US11629363B2

公开(公告)日：2023-04-18

申请号：US15873798

申请日：2018-01-17

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P7/20 ,  C12P7/24 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52 ,  C12P7/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US11085015B2

公开(公告)日：2021-08-10

申请号：US15585078

申请日：2017-05-02

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12P7/42 ,  C12P7/18 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  C08G18/32 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：US20210002673A1

公开(公告)日：2021-01-07

申请号：US16888339

申请日：2020-05-29

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US20200299736A1

公开(公告)日：2020-09-24

申请号：US16664648

申请日：2019-10-25

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P5/02 ,  C12N15/52 ,  C07C11/16 ,  C08F136/06

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US10351887B2

公开(公告)日：2019-07-16

申请号：US15634726

申请日：2017-06-27

Applicant: GENOMATICA, INC.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya ,  Mark J. Burk

IPC: C08G63/02 ,  C12P17/08 ,  C12N15/70 ,  C07D313/04 ,  C12N15/63 ,  C12P17/02 ,  C08G18/42 ,  C12N5/00 ,  C08G63/08 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：US20190106701A1

公开(公告)日：2019-04-11

申请号：US15979313

申请日：2018-05-14

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62 ,  C12N15/63 ,  C08F120/06 ,  C12P7/42 ,  C12P7/40 ,  C12P7/24 ,  C08G63/664

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.