公开(公告)号：US20150154352A1

公开(公告)日：2015-06-04

申请号：US14409452

申请日：2013-06-21

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Andrea Loehr ,  Thomas Hunt ,  Everett Hurteau Meyer ,  Walter Mathias Howell ,  Gary Withey

IPC: G06F19/22 ,  G01N33/68 ,  G01N33/50 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G16B30/00 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6881 ,  C12Q2600/16 ,  G01N33/487 ,  G01N33/5005 ,  G01N33/68 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsions or droplets. A biological sample is divided into subsamples of single cells or cell supbopulations, and a fusion complex is formed by molecular linkage and amplification techniques. Methods, apparatuses, and systems are provided for high-throughput, massively parallel analysis of the subsamples. These methods integrate molecular, algorithmic, and engineering approaches. They have broad and useful application in a number of biological and medical fields, including immunology, noninvasive prenatal diagnosis, and noninvasive cancer diagnosis.

Abstract translation: 提供了方法和系统用于在乳液或液滴中对单个细胞的大规模并行遗传分析。 将生物样品分为单细胞或细胞支原体的子样本，通过分子连锁和扩增技术形成融合复合体。 提供了方法，装置和系统用于子采样的高通量，大规模并行分析。 这些方法集成了分子，算法和工程方法。 它们在许多生物和医学领域具有广泛和有用的应用，包括免疫学，无创产前诊断和非侵入性癌症诊断。

公开(公告)号：US20230313291A1

公开(公告)日：2023-10-05

申请号：US18102125

申请日：2023-01-27

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888

CPC classification number: C12Q1/6874 ,  C12N15/1006 ,  C07K16/00 ,  C12Q1/6846 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12N15/1065 ,  C12N15/1075 ,  C12Q1/6888 ,  C07K2317/622 ,  C40B50/06

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US11702765B2

公开(公告)日：2023-07-18

申请号：US16886612

申请日：2020-05-28

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C40B40/08 ,  C07K16/00 ,  C12N15/10 ,  C07K14/725 ,  C12Q1/6876 ,  C40B50/06

CPC classification number: C40B40/08 ,  C07K14/7051 ,  C07K16/00 ,  C12N15/1037 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C07K2317/14 ,  C07K2317/622 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20220064303A1

公开(公告)日：2022-03-03

申请号：US17418776

申请日：2019-12-27

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Rena Aviva Mizrahi ,  Yoong Wearn Lim ,  Michael Asensio ,  Erica Lyn Stone

IPC: C07K16/28 ,  A61P35/00

Abstract: Provided herein are antigen-binding proteins (ABPs) that selectively bind to CTLA-4 and its isoforms and homologs, and compositions comprising the ABPs. Also provided are methods of using the ABPs, such as therapeutic and diagnostic methods.

公开(公告)号：US20210371851A1

公开(公告)日：2021-12-02

申请号：US17392892

申请日：2021-08-03

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C12N15/10 ,  C12Q1/6888 ,  C07K16/00 ,  C07K14/725 ,  C07K14/74 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US11111490B2

公开(公告)日：2021-09-07

申请号：US16443381

申请日：2019-06-17

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C40B40/08 ,  C12N15/10 ,  C12Q1/6888 ,  C07K16/00 ,  C07K14/725 ,  C07K14/74 ,  C12Q1/6869 ,  C12Q1/6806 ,  C40B50/06

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US20200291390A1

公开(公告)日：2020-09-17

申请号：US16886612

申请日：2020-05-28

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10 ,  C07K16/00 ,  C07K14/725 ,  C12Q1/6876

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20190256841A1

公开(公告)日：2019-08-22

申请号：US16399759

申请日：2019-04-30

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10 ,  C12Q1/6876 ,  C07K14/725 ,  C07K16/00

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US09926555B2

公开(公告)日：2018-03-27

申请号：US15598314

申请日：2017-05-18

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C40B50/06 ,  C12N15/10

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20170247683A1

公开(公告)日：2017-08-31

申请号：US15598313

申请日：2017-05-18

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.