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21.发明申请RNA-dependent RNA polymerase functioning preferably on a RNA matrix and promoter-dependent transcription process with said RNA-dependent RNA polymerase 有权优选在RNA基质上起作用的RNA依赖性RNA聚合酶和使用所述RNA依赖性RNA聚合酶的启动子依赖性转录过程公开(公告)号：US20050069933A1
公开(公告)日：2005-03-31
申请号：US10940964
申请日：2004-09-15
申请人： Valerie Cheynet-Sauvion , Nadege Arnaud-Barbe , Guy Oriol , William McAllister , Bernard Mandrand , Francois Mallet
发明人： Valerie Cheynet-Sauvion , Nadege Arnaud-Barbe , Guy Oriol , William McAllister , Bernard Mandrand , Francois Mallet
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/54 , C12Q1/68 , C07H21/04 , C12N9/22 , C12N15/74 , C12Q1/70
CPC分类号： C12Q1/6865 , C12N9/127 , C12Q2521/119
摘要： RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.
摘要翻译： 可以使用核苷酸试剂作为启动子来转录RNA。 通过已知DNA依赖性的RNA聚合酶，例如噬菌体T7的RNA聚合酶，或者通过新的突变的RNA聚合酶，使试剂可以使RNA不经序列指定而没有蛋白质辅因子转录， 当基质是RNA时，与基质是DNA相比，合成具有更高收率的多核苷酸基质的转录产物的能力。 可以通过对野生型RNA聚合酶的编码基因进行突变，然后通过选择具有该能力的突变的RNA聚合酶来获得这种类型的RNA聚合酶。 本发明可以应用于RNA的检测，合成或定量。
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公开(公告)号：US5817465A
公开(公告)日：1998-10-06
申请号：US825617
申请日：1997-03-31
申请人： Francois Mallet , Guy Oriol , Bernard Mandrand
发明人： Francois Mallet , Guy Oriol , Bernard Mandrand
CPC分类号： C12Q1/686 , C12Q1/6865
摘要： An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
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公开(公告)号：USRE39031E1
公开(公告)日：2006-03-21
申请号：US09680946
申请日：2000-10-06
申请人： Francois Mallet , Guy Oriol , Bernard Mandrand
发明人： Francois Mallet , Guy Oriol , Bernard Mandrand
CPC分类号： C12Q1/686 , C12Q2521/107
摘要： An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
摘要翻译： RNA扩增方法特别适用于诊断细菌或病毒感染或遗传疾病和细胞分型。 该方法包括以下步骤：使含有RNA的溶液变性，在逆转录酶存在下合成来自合适引物的第一条cDNA链，使形成的异源双链变性，在DNA聚合酶存在下从第二引物合成第二条cDNA链，然后 使形成的cDNA进行足够数量的扩增循环。 所有的反应物和溶剂首先放在同一容器中，以提供避免污染风险的单一操作步骤。
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公开(公告)号：US07060804B2
公开(公告)日：2006-06-13
申请号：US10182126
申请日：2001-01-22
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z′, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, α-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising such a complex or colloidal particles.
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25.发明授权Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers 失效使用DNA / RNA嵌合引物通过链置换扩增核酸序列的方法
公开(公告)号：US5824517A
公开(公告)日：1998-10-20
申请号：US817035
申请日：1997-05-16
CPC分类号： C12Q1/6844
摘要： A target nucleic acid may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridizing with a 3'-terminal segment of the target and a DNA-type segment capable of hybridizing with a segment adjacent to the 3'-terminal segment of the target and a DNA- or RNA-type primer capable of hybridizing with the 3'-terminal segment of the target. A cyclic amplification that may be implemented isothermally on the basis of either a DNA or an RNA target is achieved even when the terminals are not defined.
摘要翻译： PCT No.PCT / FR96 / 01166 Sec。 371日期：1997年5月16日 102（e）日期1997年5月16日PCT提交1996年7月24日PCT公布。 出版物WO97 / 04126 日期1997年2月6日目标核酸可以在包括DNA聚合酶，链转运和RNA酶H活性的酶系统存在下扩增，使用包含在5'至3'方向的RNA型的嵌合引物 能够与靶标的3'末端区段杂交的区段和能够与靶标的3'-末端区段相邻的区段杂交的DNA型区段以及能够与靶标杂交的DNA或RNA型引物 目标的3'-末端区段。 即使没有限定端子，也可以基于DNA或RNA靶标等温地进行循环扩增。
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26.发明授权Device for the capture of target molecules, and capturing process using the device 失效用于捕获目标分子的装置，以及使用该装置的捕获过程
公开(公告)号：US5723344A
公开(公告)日：1998-03-03
申请号：US264996
申请日：1994-06-24
申请人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
IPC分类号： G01N33/543 , C12Q1/68 , G01N33/544
CPC分类号： G01N33/54353 , G01N33/54366 , Y10S435/975 , Y10S436/807
摘要： Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption. When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.
摘要翻译： 用于捕获靶分子以用于检测和/或测定靶分子的装置，包括其上固定有配体的固体支持体，所述配体以由聚合物与多个共价键共价偶联得到的缀合物形式提供 的配体分子。 聚合物是N-乙烯基吡咯烷酮共聚物，并且通过吸附将缀合物固定在固体载体上。 当配体能够与目标物形成复合物时，该装置对于给定的靶标是特异性的。 此外，当该装置另外包含能够与配体一起形成络合物的双功能试剂，另一方面，另外，与靶标配位的配体固定的载体构成通用捕获系统。
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公开(公告)号：US07879594B2
公开(公告)日：2011-02-01
申请号：US10548869
申请日：2004-03-15
IPC分类号： C12M1/00
CPC分类号： B82Y5/00 , C12Q1/6825 , C12Q1/6837 , C12Q2563/125 , C12Q2565/113 , C12Q2565/607 , G01N33/5438 , Y10S977/70 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/72
摘要： The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.
摘要翻译： 本发明涉及用于检测包含多个生物分子的样品中的目标生物分子的方法，由此所述靶生物分子被提供有标签，所述标签包含催化活性部分，其催化产生不溶性反应产物的反应，所述反应产物在 柔性导电纳米电极。 所述纳米电极上的沉淀导致其电导率的变化，其可用于电分析方法。 本发明进一步涉及一种生物芯片，其包括固体支持体，其上附着有纳米电极，以及探针分子结合到全部或多个所述纳米电极上，所述纳米电极可以相同或不同，所述探针分子的一段能够与片段 的目标生物分子。
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28.发明申请Novel Electropolymerisable Monomers, Soluble in an Aqueous Solution and Comprising a Metalloporphyrin 失效新型可电聚合单体，可溶于水溶液并包含金属卟啉公开(公告)号：US20090314660A1
公开(公告)日：2009-12-24
申请号：US12084476
申请日：2006-11-02
申请人： Frédéric Canonne , Hafsa Korri-Youssoufi , Jean-Pierre Mahy , Bernard Mandrand , Martine Perree-Fauvet
发明人： Frédéric Canonne , Hafsa Korri-Youssoufi , Jean-Pierre Mahy , Bernard Mandrand , Martine Perree-Fauvet
CPC分类号： C08G61/124 , C07D487/22 , G01N27/48
摘要： The invention relates to novel electropolymerisable monomers which are to be polymerised in an aqueous solution and comprise: an electropolymerisable pattern selected from acetylene, pyrrols, thiophenes, indols, anilines, azines, p-phenylene vinylenes, p-phenylenes, pyrenes, furanes, selenophenes, pyrridazines, carbazoles, acrylates, methacrylates and the derivatives thereof, and a metalloporphyrine which is substituted by at least two ionised or ionisable entities in an aqueous solution. The invention also relates to a method for the polymerisation of such monomers, to the electroactive probe that can be obtained by the polymerisation of such monomers, and to a method for detecting a target ligand in a biological sample using one such electroactive probe.
摘要翻译： 本发明涉及要在水溶液中聚合的新型可电聚合单体，包括：可电聚合图案，其选自乙炔，吡咯，噻吩，吲哚，苯胺，吖嗪，对亚苯基乙烯，对亚苯基，芘，呋喃，硒吩 吡咯嗪，咔唑，丙烯酸酯，甲基丙烯酸酯及其衍生物，以及在水溶液中由至少两个电离或电离实体取代的金属卟啉。 本发明还涉及这种单体的聚合方法，可以通过这些单体的聚合获得的电活性探针，以及使用一种这样的电活性探针检测生物样品中的靶配体的方法。
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29.发明授权RNA-dependent RNA polymerase functioning preferably on a RNA matrix and promoter-dependent transcription process with said RNA-dependent RNA polymerase 有权优选在RNA基质上起作用的RNA依赖性RNA聚合酶和使用所述RNA依赖性RNA聚合酶的启动子依赖性转录过程公开(公告)号：US07595180B2
公开(公告)日：2009-09-29
申请号：US10940964
申请日：2004-09-15
申请人： Valérie Cheynet-Sauvion , Nadège Arnaud-Barbe , Guy Oriol , William McAllister , Bernard Mandrand , François Mallet
发明人： Valérie Cheynet-Sauvion , Nadège Arnaud-Barbe , Guy Oriol , William McAllister , Bernard Mandrand , François Mallet
CPC分类号： C12Q1/6865 , C12N9/127 , C12Q2521/119
摘要： RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.
摘要翻译： 可以使用核苷酸试剂作为启动子来转录RNA。 通过已知DNA依赖性的RNA聚合酶，例如噬菌体T7的RNA聚合酶，或者通过新的突变的RNA聚合酶，使试剂可以使RNA不经序列指定而没有蛋白质辅因子转录， 当基质是RNA时，与基质是DNA相比，合成具有更高收率的多核苷酸基质的转录产物的能力。 可以通过对野生型RNA聚合酶的编码基因进行突变，然后通过选择具有该能力的突变的RNA聚合酶来获得这种类型的RNA聚合酶。 本发明可以应用于RNA的检测，合成或定量。
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公开(公告)号：US20070037153A1
公开(公告)日：2007-02-15
申请号：US10548869
申请日：2004-03-15
CPC分类号： B82Y5/00 , C12Q1/6825 , C12Q1/6837 , C12Q2563/125 , C12Q2565/113 , C12Q2565/607 , G01N33/5438 , Y10S977/70 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/72
摘要： The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.
摘要翻译： 本发明涉及用于检测包含多个生物分子的样品中的靶生物分子的方法，由此所述靶生物分子被提供有标签，所述标签包含催化活性部分，其催化产生不溶性反应产物的反应，所述反应产物在 柔性导电纳米电极。 所述纳米电极上的沉淀导致其电导率的变化，其可用于电分析方法。 本发明进一步涉及一种生物芯片，其包括固体支持体，其上附着有纳米电极，并且探针分子结合到所有或多个所述纳米电极上，所述纳米电极可以相同或不同，所述探针分子的一段能够与片段 的目标生物分子。
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