利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.022 秒)

    Method for manufacturing a sensor chip and sensor chip blank
    21.
    发明申请
    Method for manufacturing a sensor chip and sensor chip blank 审中-公开
    制造传感器芯片和传感器芯片空白的方法

    公开(公告)号：US20060141445A1

    公开(公告)日：2006-06-29

    申请号：US11316674

    申请日：2005-12-22

    申请人： Holger Klapproth

    发明人： Holger Klapproth

    IPC分类号： C12Q1/00

    CPC分类号： G01N33/54353 ,  G01N33/54393

    摘要： In a process for manufacturing a sensor chip a support is prepared having at least one surface region in which at least one reactive group is arranged. A buffer solution is applied to the surface region for coating at least one surface region. Then, at least one receptor having binding specificity for a ligand to be detected is brought into contact with the surface coating so obtained in such a manner that said receptor binds covalently to at least one reactive group.

    摘要翻译： 在制造传感器芯片的方法中，制备具有至少一个表面区域的载体，其中布置有至少一个反应性基团。 将缓冲溶液施加到表面区域以涂覆至少一个表面区域。 然后，使具有对待检测配体具有结合特异性的至少一种受体与如此获得的表面涂层接触，使得所述受体共价结合至至少一个反应性基团。

    Sensor surface comprising an improved signal-noise ratio
    22.
    发明申请
    Sensor surface comprising an improved signal-noise ratio 审中-公开
    传感器表面包括改善的信噪比

    公开(公告)号：US20060115906A1

    公开(公告)日：2006-06-01

    申请号：US10521817

    申请日：2003-07-21

    申请人： Holger Klapproth

    发明人： Holger Klapproth

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54353 ,  G01N33/54373 ,  G01N33/54393

    摘要： The present invention concerns a sensor surface with an improved signal/noise ratio that is based on a blocking reagent covalently immobilized on the sensor surface, as well as apparatuses comprising such surfaces and methods for the use of same.

    摘要翻译： 本发明涉及一种基于共价固定在传感器表面上的封闭剂，具有改进的信噪比的传感器表面以及包括这些表面的设备及其使用方法。

    Composition and process for stabilizing of biomolecules
    23.
    发明授权
    Composition and process for stabilizing of biomolecules 失效
    用于稳定生物分子的组成和方法

    公开(公告)号：US07563762B2

    公开(公告)日：2009-07-21

    申请号：US10520158

    申请日：2003-07-04

    申请人： Holger Klapproth

    发明人： Holger Klapproth

    IPC分类号： A61K38/00 ,  A61K9/16 ,  A61K31/715 ,  C12P19/12

    CPC分类号： C07K17/00 ,  C07K14/00

    摘要： The present invention relates in general to the field of biotechnology. The invention particularly relates to a composition and process for stabilizing or preserving biological molecules, as well as devices that comprise correspondingly stabilized or preserved biomolecules.

    摘要翻译： 本发明一般涉及生物技术领域。 本发明特别涉及用于稳定或保存生物分子的组合物和方法，以及包含相应稳定或保留的生物分子的装置。

    Kit and Device for Generating Chemiluminescence Radiation
    24.
    发明申请
    Kit and Device for Generating Chemiluminescence Radiation 审中-公开
    用于生成化学发光辐射的试剂盒和装置

    公开(公告)号：US20090093047A1

    公开(公告)日：2009-04-09

    申请号：US12182676

    申请日：2008-07-30

    申请人： Holger Klapproth ,  Sonja Bender Mohry

    发明人： Holger Klapproth ,  Sonja Bender Mohry

    IPC分类号： C12M1/34

    CPC分类号： G01N21/76 ,  G01N21/05 ,  G01N33/54386 ,  G01N33/581 ,  G01N2333/902

    摘要： A device for generating a chemiluminescence radiation depending on the binding of at least one ligand that is contained in a sample that is to be tested to at least one receptor that is binding-specific for the ligand has a flow cell having an internal cavity. The internal cavity has a separating wall on which the at least one receptor is immobilized. The internal cavity has at least two compartments. In a first compartment solid is deposited that contains at least one peroxidase-enzyme-possessing marker for marking the ligand in lyophilized form. A second compartment contains salt-stabilized luminol and a solid substance that releases hydrogen peroxide upon contact with water.

    摘要翻译： 根据待测样品中所含的至少一种配体与至少一种对配体结合特异性的受体的结合产生化学发光辐射的装置具有具有内腔的流动池。 内腔具有分离壁，其上固定有至少一种受体。 内腔具有至少两个隔室。 在第一隔室中沉积含有至少一种含有冻干形式标记配体的过氧化物酶 - 酶标记物。 第二个隔间含有盐稳定的鲁米诺和与水接触时释放过氧化氢的固体物质。

    Method and device for determining a concentration of ligands in an analysed sample
    28.
    发明授权
    Method and device for determining a concentration of ligands in an analysed sample 失效
    用于测定分析样品中配体浓度的方法和装置

    公开(公告)号：US08685644B2

    公开(公告)日：2014-04-01

    申请号：US10515535

    申请日：2003-11-04

    申请人： Holger Klapproth

    发明人： Holger Klapproth

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

    CPC分类号： G01N33/54373 ,  B01L3/5027 ,  G01N27/4145 ,  G01N2035/00158

    摘要： The invention relates to a method for determining a concentration of ligands in an analyzed sample. The inventive method consists in immobilizing receptors specifically boundable with said ligands on a support. At least one measured value of the occupation of the support surface by the receptors is determined by means of a sensor for the surface occupation. Afterwards, the sample is put in contact with the receptors. At least one measured value of the frequency of liaisons between the ligands and the receptors is determined by means of at least one detector. The concentration of ligands in the sample can be determined with the aid of the measured values of the occupation of the surface and frequency of ligands/receptors liaisons.

    摘要翻译： 本发明涉及一种用于测定分析样品中配体浓度的方法。 本发明的方法包括将与所述配体特异性结合的受体固定在载体上。 通过用于表面占据的传感器来确定由受体对支撑表面的占据的至少一个测量值。 然后将样品与受体接触。 通过至少一个检测器确定配体和受体之间的联络频率的至少一个测量值。 样品中配体的浓度可以借助于配体/受体结构的表面和频率的占据的测量值来确定。

    Method For Covalently Immobilising Biomolecules on Organic Surfaces
    29.
    发明申请
    Method For Covalently Immobilising Biomolecules on Organic Surfaces 审中-公开
    共价固定有机表面生物分子的方法

    公开(公告)号：US20080293592A1

    公开(公告)日：2008-11-27

    申请号：US10591064

    申请日：2005-03-01

    申请人： Jurgen Ruhe ,  Holger Klapproth

    发明人： Jurgen Ruhe ,  Holger Klapproth

    IPC分类号： C40B40/04 ,  C40B50/18

    CPC分类号： G01N33/54353 ,  C12N11/06 ,  C12N11/08 ,  G01N33/54366

    摘要： The invention relates to a method for covalently immobilising probe-biomolecules on organic surfaces by means of photoreactive cross-linking agents which are used for covalently immobilising the probe-biomolecules on an organic surface. The inventive immobilising method consists in applying said probe-biomolecules and photoactive polymers and afterwards in cross-linking.

    摘要翻译： 本发明涉及通过用于共价固定探针 - 生物分子在有机表面上的光反应性交联剂将探针 - 生物分子共价固定在有机表面上的方法。 本发明的固定方法包括应用所述探针 - 生物分子和光活性聚合物，然后在交联中。

    Microoptical Detection System and Method for Determination of Temperature-Dependent Parameters of Analytes
    30.
    发明申请
    Microoptical Detection System and Method for Determination of Temperature-Dependent Parameters of Analytes 审中-公开
    微光学检测系统和测定分析物温度依赖参数的方法

    公开(公告)号：US20080160548A1

    公开(公告)日：2008-07-03

    申请号：US11910600

    申请日：2006-04-13

    申请人： Holger Klapproth

    发明人： Holger Klapproth

    IPC分类号： G01N33/00 ,  G01N21/76 ,  C12M1/00

    CPC分类号： G01N33/54373 ,  G01N33/557 ,  G01N2035/00158

    摘要： The invention relates to a microoptical detection system and a method for detecting analytes by means of time-resolved luminescence. This serves for determination of temperature-dependent parameters of analytes, in particular for determination of point mutations of nucleic acids (DNA), for which a time-resolved detection is required.

    摘要翻译： 本发明涉及一种微光学检测系统和通过时间分辨发光检测分析物的方法。 这用于测定分析物的温度依赖性参数，特别是用于确定需要时间分辨检测的核酸（DNA）的点突变。