公开(公告)号：US09050422B2

公开(公告)日：2015-06-09

申请号：US13385951

申请日：2012-03-15

申请人： Philip H. Coelho ,  Bruce A. Baker ,  John R. Chapman ,  Junzhi Li ,  Prince Emmanuel ,  Robert S. Childers

发明人： Philip H. Coelho ,  Bruce A. Baker ,  John R. Chapman ,  Junzhi Li ,  Prince Emmanuel ,  Robert S. Childers

IPC分类号： A61M1/36 ,  B04B5/00 ,  A61M1/02 ,  B04B5/04 ,  B01D21/34 ,  B01D21/26

CPC分类号： A61M1/3693 ,  A61B5/15003 ,  A61B5/150038 ,  A61B5/150045 ,  A61B5/150366 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/153 ,  A61M1/0209 ,  A61M1/0231 ,  A61M1/029 ,  A61M2202/0462 ,  B01D21/262 ,  B01D21/34 ,  B01D2221/10 ,  B04B5/0428

摘要： The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.

摘要翻译： 本发明包括从含有大多数活的CD34 +细胞的骨髓或脐血回收的干细胞和祖细胞的组合物，并且基本上不存在驻留在原始样品中的红细胞，而没有任何异生生物添加剂来辅助细胞分离。 本发明还包括用于制备组合物的系统和方法。 该系统包括一个采用光学传感器，微控制器，伺服电机，加速度计，称重传感器和电池的行李箱和处理装置。 该系统和方法利用离心将细胞分层，然后分离并将干细胞转移到干细胞袋中。 处理设备的微控制器接收来自设备的加速度计，称重传感器和光学传感器的输入，以将计量阀引导到袋组中打开和关闭，以允许尽可能少的红细胞转移尽可能多的茎细胞。

公开(公告)号：US20120171762A1

公开(公告)日：2012-07-05

申请号：US13385951

申请日：2012-03-15

申请人： Philip H. Coelho ,  Bruce A. Baker ,  John R. Chapman ,  Junzhi Li ,  Prince Emmanuel ,  Robert S. Childers

发明人： Philip H. Coelho ,  Bruce A. Baker ,  John R. Chapman ,  Junzhi Li ,  Prince Emmanuel ,  Robert S. Childers

IPC分类号： C12N5/0789

CPC分类号： A61M1/3693 ,  A61B5/15003 ,  A61B5/150038 ,  A61B5/150045 ,  A61B5/150366 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/153 ,  A61M1/0209 ,  A61M1/0231 ,  A61M1/029 ,  A61M2202/0462 ,  B01D21/262 ,  B01D21/34 ,  B01D2221/10 ,  B04B5/0428

摘要： The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.

公开(公告)号：US08177072B2

公开(公告)日：2012-05-15

申请号：US12315722

申请日：2008-12-04

申请人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Phillip D. Kingsley

发明人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Phillip D. Kingsley

IPC分类号： B01D21/26

CPC分类号： B01D21/26 ,  A61M1/029 ,  A61M2202/0413 ,  A61M2202/0464 ,  A61M2202/10 ,  B01D21/262 ,  B01D21/307 ,  B01D21/34 ,  B01D2221/10 ,  B01L3/50215 ,  B01L2300/048 ,  B01L2300/049 ,  B01L2300/0681 ,  B01L2400/0605 ,  B01L2400/0611 ,  G01N2035/00495

摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

公开(公告)号：US20100140182A1

公开(公告)日：2010-06-10

申请号：US12315722

申请日：2008-12-04

申请人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Philip D. Kingsley

发明人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Philip D. Kingsley

IPC分类号： B01D21/34 ,  B01D21/26

CPC分类号： B01D21/26 ,  A61M1/029 ,  A61M2202/0413 ,  A61M2202/0464 ,  A61M2202/10 ,  B01D21/262 ,  B01D21/307 ,  B01D21/34 ,  B01D2221/10 ,  B01L3/50215 ,  B01L2300/048 ,  B01L2300/049 ,  B01L2300/0681 ,  B01L2400/0605 ,  B01L2400/0611 ,  G01N2035/00495

摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

摘要翻译： 一种用于分离和分离生物流体组分的装置，包括用于容纳待处理流体的容器，用于在提供与其进行填充和抽出连通的同时关闭容器的管帽组件;设置在容器内用于漏斗和控制生物 流体流入浮子内的反向圆顶形隔离室，并控制生物流体流出隔离室，用于在离心过程中进行隔离室内至少一种组分或分离的生物流体流的密封隔离 处理。 所述装置还包括用于连接设置在所述浮子组件内的抽吸通道的柔性管和所述管帽组件的抽吸阀，用于允许至少提取在所述室内封装或隔离的所述一个部件或部件。

公开(公告)号：US06190609B1

公开(公告)日：2001-02-20

申请号：US09081168

申请日：1998-05-18

申请人： John R. Chapman ,  Peter R. H. Stark ,  Michael V. Swallow ,  Dale N. Larson

发明人： John R. Chapman ,  Peter R. H. Stark ,  Michael V. Swallow ,  Dale N. Larson

IPC分类号： A61L200

CPC分类号： A61M1/3681 ,  A61L2/0011 ,  A61M1/3683

摘要： Apparatus and methods for treating a biological fluid with light and for inactivating contaminants in biological fluid. The biological fluid is contacted with a light source providing a high intensity light to the biological fluid. The biological fluid may include a quantity of a photochemical agent that when activated by light is operable to cause inactivation of at least some of the contaminants.

摘要翻译： 用于用光处理生物流体并灭活生物流体中的污染物的装置和方法。 生物流体与向生物流体提供高强度光的光源接触。 生物流体可以包括一定量的光化学试剂，当光被活化时，其可操作地使至少一些污染物失活。

公开(公告)号：US5976884A

公开(公告)日：1999-11-02

申请号：US222518

申请日：1998-12-29

申请人： John R. Chapman ,  Jean M Mathias

发明人： John R. Chapman ,  Jean M Mathias

IPC分类号： A61M1/36 ,  A61K31/00 ,  A61K31/54 ,  A61K31/5415 ,  A61K41/00 ,  A61P31/00 ,  A61P31/12 ,  G01N21/75 ,  G01N21/78

CPC分类号： A61K41/0019 ,  Y10S436/904 ,  Y10S436/905

摘要： Systems and methods treat biological fluids carrying contaminants by photoactivating materials mixed with the fluids. The systems and methods quantify the photoreactions that occur in a manner that permits correlation with clinical results.

摘要翻译： 系统和方法通过光活化与流体混合的材料来处理携带污染物的生物流体。 系统和方法定量以允许与临床结果相关的方式发生的光反应。

公开(公告)号：US5765615A

公开(公告)日：1998-06-16

申请号：US717546

申请日：1996-09-20

申请人： John R. Chapman ,  Ernie R. Buchacher

发明人： John R. Chapman ,  Ernie R. Buchacher

IPC分类号： B27B1/00 ,  B27L11/00 ,  B27C9/00

CPC分类号： B27B1/007 ,  B27L11/007

摘要： A canter capable of curve-cutting featuring rotary, power-driven frustro-conical chipper heads for chipping a workpiece, which chipper heads are adjustable under the influence of movement structure to achieve five degrees of freedom of motion and adjustment which take into account the different topographical characteristics of different workpieces.

摘要翻译： 一个能够进行曲线切割的车轮，具有旋转，动力驱动的截头圆锥形切屑头，用于切削工件，该切屑头在运动结构的影响下是可调节的，以实现运动和调整的五个自由度，其考虑到不同的 不同工件的地形特征。

公开(公告)号：US5641622A

公开(公告)日：1997-06-24

申请号：US255107

申请日：1994-06-07

申请人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

发明人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12Q1/24 ,  G01N33/50 ,  G01N33/569 ,  A01N1/02 ,  C12N1/02 ,  C12Q1/68

CPC分类号： G01N33/56972 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.

摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。

公开(公告)号：US5498520A

公开(公告)日：1996-03-12

申请号：US037525

申请日：1993-03-24

申请人： John R. Chapman

发明人： John R. Chapman

IPC分类号： B01D17/02 ,  B01D21/24 ,  B01D21/26 ,  C12N7/00 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/49 ,  G01N33/50 ,  G01N33/53 ,  G01N33/569 ,  C12P19/34

CPC分类号： G01N33/56972 ,  B01D17/0217 ,  B01D21/0012 ,  B01D21/2405 ,  B01D21/2444 ,  B01D21/245 ,  B01D21/26 ,  C12Q1/6806 ,  C12Q1/70 ,  G01N33/491 ,  G01N33/5091 ,  G01N33/5094 ,  B01D2221/10

摘要： The present invention provides a method for testing a blood unit for viral contamination without rendering the blood unit unusable for therapeutic applications. The method comprises the steps of: removing and collecting from a blood unit a majority of the leukocytes present therein with a leukocyte filter; and using the leukocytes collected in and on the filter to test the blood unit for viral contamination. The present invention also provides a method for validating viral inactivation processes.

摘要翻译： 本发明提供了一种用于测试用于病毒污染的血液单元的方法，而不使得血液单元不能用于治疗应用。 该方法包括以下步骤：用白细胞过滤器从血液单元中除去和收集大多数存在于其中的白细胞; 并使用收集在过滤器中和过滤器上的白细胞来测试血液单位以进行病毒感染。 本发明还提供了用于验证病毒灭活过程的方法。

公开(公告)号：US09101926B2

公开(公告)日：2015-08-11

申请号：US13199119

申请日：2011-08-19

申请人： John R. Chapman ,  Rodney Sparks ,  Philip H. Coelho

发明人： John R. Chapman ,  Rodney Sparks ,  Philip H. Coelho

IPC分类号： B01L3/00 ,  G01N33/49 ,  A61M1/36

CPC分类号： B04B5/0414 ,  A61M1/3693 ,  B01D21/262 ,  B01L3/5021 ,  B01L2300/0851 ,  B01L2400/0409 ,  B04B1/00 ,  B04B5/02 ,  G01N33/491

摘要： The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

摘要翻译： 离心容器包括包含内部空间的外壁。 大坝定义了将内部空间分成至少两个区域的障碍物，所述至少两个区域包括限定更高的gee区域的捕集池和限定较低的gee区域的储存器。 这些地区通过大坝连接在一起。 大坝包括一个优选为锥形的面，以便优化放置在容器内的样品的分离速度。 该容器可用于生物样品处理方法，通过使容器的较高gee区域配置为具有细长形式，并且为了收集样品的较高密度部分而优化的体积。 供应和取出管延伸到区域以进行可靠的提取，并通过离心分离后分离收集不同密度的馏分。