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32 条记录 (耗时 0.028 秒)

    Biosynthetic human growth hormone
    22.
    发明授权
    Biosynthetic human growth hormone 失效
    生物合成人生长激素

    公开(公告)号：US5633352A

    公开(公告)日：1997-05-27

    申请号：US402286

    申请日：1995-03-10

    申请人： Henrik Dalb.o slashed.ge ,  John Pedersen ,  Thorkild Christensen ,  J.o slashed.rli W. Ringsted ,  Torben E. Jessen

    发明人： Henrik Dalb.o slashed.ge ,  John Pedersen ,  Thorkild Christensen ,  J.o slashed.rli W. Ringsted ,  Torben E. Jessen

    IPC分类号： C07K14/545 ,  C07K14/61 ,  C12N9/22 ,  C12N9/36 ,  C12N15/62 ,  C12P21/06

    CPC分类号： C12N9/2462 ,  C07K14/545 ,  C07K14/61 ,  C12N15/62 ,  C12N9/22 ,  C12P21/06 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75

    摘要： A desired protein having the formula:A-B-C-Pwhereina) A is Lys or Arg, and B and C are arbitrary amino acids, orb) A is an arbitrary amino acid different from Pro, Lys and Arg, and B and/or C is Pro,is produced from a biosynthetically formed amino acid extended protein having the formula:X-A-B-C-Pwherein A, B, C and P are as defined above, and X is an amino acid sequence with an even number of amino acids, of which the first one, seen from the N-terminal end, is different from Lys and Arg, all other uneven amino acids are different from Pro, Lys and Arg, and all even amino acids are different from Pro, by reaction with the enzyme dipeptidyl aminopeptidase (DAP I). The desired protein is obtained in a pure state. Thus, e.g. hGH without content of Met-hGH may be produced by the process.

    摘要翻译： 具有下式的所需蛋白质：ABC-P，其中a）A是Lys或Arg，B和C是任意氨基酸，或b）A是不同于Pro，Lys和Arg的任意氨基酸，B和/或C Pro是由具有下式的生物合成形成的氨基酸延伸蛋白产生：XABC-P，其中A，B，C和P如上所定义，X是具有偶数氨基酸的氨基酸序列，其中 第一个，从N末端看，与Lys和Arg不同，所有其他不均匀的氨基酸与Pro，Lys和Arg不同，所有偶联氨基酸与Pro不同，通过与酶二肽基氨基肽酶（ DAP I）。 在纯状态下获得所需的蛋白质。 因此，例如 可以通过该过程产生没有Met-hGH含量的hGH。

    Enzyme with .beta.-(1-6)- endoglucanase activity
    29.
    发明授权
    Enzyme with .beta.-(1-6)- endoglucanase activity 失效
    具有β-（1-6） - 内切葡聚糖酶活性的酶

    公开(公告)号：US6022723A

    公开(公告)日：2000-02-08

    申请号：US40699

    申请日：1998-03-18

    申请人： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Henrik Dalb.o slashed.ge ,  Hans Sejr Olsen

    发明人： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Henrik Dalb.o slashed.ge ,  Hans Sejr Olsen

    IPC分类号： C12G1/00 ,  A23L2/52 ,  C12N1/14 ,  C12N9/24 ,  C12N15/00 ,  C12N15/09 ,  C12P21/00 ,  C12R1/685 ,  C12R1/69 ,  C12R1/885 ,  C12N1/00 ,  C12N1/20 ,  C12P21/04

    CPC分类号： C12Y302/01075 ,  C12N9/2402 ,  Y10S435/945

    摘要： The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译： 本发明涉及由DNA序列编码的β-（1-6） - 内葡聚糖酶活性的酶，该DNA序列a）包含SEQ ID No.3所示的DNA序列，或b）包含DNA的类似物 序列号3所示的序列，其i）与SEQ ID No.3所示的DNA序列同源，和/或ii）与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交， 和/或iii）编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽，和/或iv）编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-（1-6） - 葡聚糖酶，如SEQ ID No.4所示，源自哈茨木霉，CBS 243.71。 本发明还涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。