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21.发明授权公开(公告)号：US08158390B2
公开(公告)日：2012-04-17
申请号：US11663984
申请日：2005-08-19
申请人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
发明人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
CPC分类号： C12P13/08 , C12N9/1096
摘要： The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacterium glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.
摘要翻译： 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。
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公开(公告)号：US20110039313A1
公开(公告)日：2011-02-17
申请号：US12517923
申请日：2008-01-10
申请人： Stefan Verseck , Harald Haeger , Andreas Karau , Lothar Eggeling , Hermann Sahm
发明人： Stefan Verseck , Harald Haeger , Andreas Karau , Lothar Eggeling , Hermann Sahm
CPC分类号： C12N9/88 , C12P13/001
摘要： The invention relates to recombinant microorganisms in which polynucleotides which code for lysine decarboxylase are enhanced and, using which, cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw materials such as, for example, glucose, sucrose, molasses and the like.
摘要翻译： 本发明涉及重组微生物，其中编码赖氨酸脱羧酶的多核苷酸被增强，并且使用其发酵生成尸胺（1,5-二氨基戊烷），所用的碳源优选是可再生原料，例如葡萄糖 ，蔗糖，糖蜜等。
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公开(公告)号：US07632663B1
公开(公告)日：2009-12-15
申请号：US09914006
申请日：2000-02-21
申请人： Lothar Eggeling , Hermann Sahm
发明人： Lothar Eggeling , Hermann Sahm
CPC分类号： C12N9/93 , C12N9/1014 , C12N9/88 , C12P13/06
摘要： The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.
摘要翻译： 本发明涉及微生物生产L-缬氨酸的方法，其中二羟基酸合酶（ilvD）活性和/或ilvD基因表达在微生物中增强。 作为替代或与其结合，在微生物中增强乙酰羟酸合酶（ilvBN）活性和异构还原酶（ilvC）活性和/或ilvBNC基因表达。 根据本发明的方法优选利用其中减少或消除了减少L-缬氨酸形成的参与代谢途径的至少一种酶的活性的微生物。 因此，例如，根据本发明的方法优选利用在苏氨酸脱水酶（ilvA）基因中具有缺陷突变的微生物和/或泛酸合成的一种或多种基因中的缺陷突变。
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24.发明申请NUCLEOTIDE SEQUENCES OF CORYNEFORM BACTERIA CODED FOR PROTEINS PARTICIPATING IN L-SERINE METABOLISM AND METHOD FOR MICROBIAL PRODUCTION OF L-SERINE 失效编码用于蛋白质参与L-丝氨酸代谢的CORYNEFORM细菌的核苷酸序列和用于微生物生产L-丝氨酸的方法公开(公告)号：US20090061482A1
公开(公告)日：2009-03-05
申请号：US12136190
申请日：2008-06-10
摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
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25.发明授权公开(公告)号：US07267967B1
公开(公告)日：2007-09-11
申请号：US09529043
申请日：1998-09-30
摘要： A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.
摘要翻译： 公开了具有SEQ ID NO：1的丙酮酸羧化酶基因以及由丙酮酸羧酸酯基因表达的具有SEQ ID NO：2的氨基酸序列。 还公开了一种微生物生产天冬氨酸和/或谷氨酸家族的氨基酸的方法，其中丙酮酸羧化酶活性通过遗传改变具有SEQ ID NO：1的丙酮酸羧化酶基因而增加，通过表达产生相应氨基的微生物 具有SEQ ID NO：2的酸。
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26.发明申请Nucleotide sequences of coryneform bacteria coding for proteins involved in l-serine metabolism and method for producing l-serine 审中-公开编码参与l-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于产生l-丝氨酸的方法公开(公告)号：US20060204963A1
公开(公告)日：2006-09-14
申请号：US10549262
申请日：2004-02-12
摘要： The invention relates to the nucleotide sequences of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase. Said invention also relates to micro-organisms and to methods for producing L-serine.
摘要翻译： 本发明涉及编码与减少和关闭L-丝氨酸脱水酶的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 所述发明还涉及微生物和生产L-丝氨酸的方法。
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公开(公告)号：US20060014259A9
公开(公告)日：2006-01-19
申请号：US10091342
申请日：2002-03-06
申请人： Kevin Burke , Hermann Sahm , Lothar Eggeling , Bernd Moritz , L. Dunican , Ashling McCormack , Cliona Stapelton , Bettina Mockel , Georg Thierbach , Rita Dunican
发明人： Kevin Burke , Hermann Sahm , Lothar Eggeling , Bernd Moritz , L. Dunican , Ashling McCormack , Cliona Stapelton , Bettina Mockel , Georg Thierbach , Rita Dunican
CPC分类号： C12N9/0006 , C12N15/77 , C12P13/08 , C12Y101/01049
摘要： The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
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28.发明申请Method for the fermentative production of L-amino acids, using coryneform bacteria 审中-公开使用棒状细菌发酵生产L-氨基酸的方法公开(公告)号：US20050079588A1
公开(公告)日：2005-04-14
申请号：US10941920
申请日：2004-09-16
申请人： Georg Sindelar , Volker Wendisch , Hermann Sahm
发明人： Georg Sindelar , Volker Wendisch , Hermann Sahm
CPC分类号： C12P13/08
摘要： L-amino acid is produced by fermenting a medium using coryneform bacteria in which one or more of the genes linked with a nitrogen metabolism and selected from the group consisting of amt, ocd, soxA and sumT is/are amplified.
摘要翻译： 使用棒状细菌发酵培养基来生产L-氨基酸，其中一个或多个与氮代谢连接的基因并选自amt，ocd，soxA和sumT的基因被扩增。
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公开(公告)号：US06680186B2
公开(公告)日：2004-01-20
申请号：US09829931
申请日：2001-04-11
IPC分类号： C12N910
CPC分类号： C12N9/1029 , C12P13/04 , C12R1/13 , C12R1/15
摘要： The present invention relates to polynucleotides corresponding to the plsC gene and which encode 1-acyl-SN-glycerol-3-phosphate acyltransferase, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having 1-acyl-SN-glycerol-3-phosphate acyltransferase activity.
摘要翻译： 本发明涉及对应于plsC基因并且编码1-酰基-SN-甘油-3-磷酸酰基转移酶的多核苷酸，产生L-氨基酸的方法以及筛选编码具有1-酰基-SN的蛋白质的多核苷酸的方法 甘油-3-磷酸酰基转移酶活性。
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公开(公告)号：US06638753B2
公开(公告)日：2003-10-28
申请号：US09845248
申请日：2001-05-01
IPC分类号： C12N910
CPC分类号： C12N9/1007 , C12P13/08 , C12P13/14
摘要： The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.
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