摘要:
The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.
摘要:
A modified porous anodic alumina template (PAA) containing a thin CNT catalyst layer directly embedded into the pore walls. CNT synthesis using the template selectively catalyzes SWNTs and DWNTs from the embedded catalyst layer to the top PAA surface, creating a vertical CNT channel within the pores. Subsequent processing allows for easy contact metallization and adaptable functionalization of the CNTs and template for a myriad of applications.
摘要:
This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.
摘要:
The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.
摘要:
A device for processing fluids includes a filter to capture a cell sample. The filter has a physical barrier to isolate the cell sample on the filter. Growth detection circuitry is associated with the filter. The growth detection circuitry identifies, through electrical measurements, a cell growth rate and hence the presence of live cells associated with the cell sample.
摘要:
A modified porous anodic alumina template (PAA) containing a thin CNT catalyst layer directly embedded into the pore walls. CNT synthesis using the template selectively catalyzes SWNTs and DWNTs from the embedded catalyst layer to the top PAA surface, creating a vertical CNT channel within the pores. Subsequent processing allows for easy contact metallization and adaptable functionalization of the CNTs and template for a myriad of applications.
摘要:
A method of making a pressure sensor or acoustic transducer having high sensitivity and reduced size. A thin sensing diaphragm is produced by growing a single crystal, highly doped silicon layer on a substrate using a chemical vapor deposition process. The diaphragm is incorporated into a pressure sensor or acoustic transducer which detects pressure variations by a change in the capacitance of a capacitor which includes the diaphragm as a movable member. The thin diaphragm produces a highly sensitive device which can be fabricated in a smaller size than sensors or transducers having thicker diaphragms.
摘要:
A semiconductor integrated circuit having a lateral bipolar transistor, is fabricated in a manner compatible with sub-micron CMOS processing. A base contact structure is formed over a bipolar active area, in essentially direct contact to a portion of the upper surface of the active region, essentially concurrent to the formation of a gate electrode on a gate dielectric layer in a CMOS active area. Sidewall spacers, adjacent the base contact region, are formed and a base region formed under the base contact structure using an oblique angle implantation. Emitter region and collector contact regions are formed concurrent with CMOS source and drain regions. An optional, oblique angle collector implant can be performed where desired.
摘要:
An isolation method for separating active regions in a semiconductor substrate by combining field oxide formation with trench isolation is disclosed. Deep trenches are etched in a silicon substrate. An oxide layer is deposited over the entire substrate such that the oxide layer also fills the trenches that have been etched. Next, a layer of polysilicon is deposited over the wafer and etched back to form polysilicon spacers. These polysilicon spacers are used to align a photoresist mask that is used to etch the oxide overlying the active regions of the substrate, thereby resulting in fully planarized isolation regions with fully walled active regions.
摘要:
A process for forming field effect transistors having self-aligned source/drain contact includes: forming an gate overlying a portion of a semiconductor; forming a first sidewall spacer on the gate; forming a source/drain region in the semiconductor; depositing a conductive layer over the semiconductor so that a step is formed in the conductive layer in a region overlying the gate and the first sidewall spacer; forming a second sidewall spacer on the step; forming a protective layer over a portion of the conducting layer not covered by the second sidewall spacer; removing the second sidewall spacer to expose a portion of the conductive layer but leave covered a portion of the conductive layer underlying the protective layer; and removing the exposed portion of the conductive layer to leave a portion of the conductive layer in contact with the source/drain region and electrically isolated from the gate. The portion of the conductive layer left is the self-aligned contact. Typically, the conductive layer is polysilicon but may alternatively be polysilicide or silicide.