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    Microfluidic protein crystallography techniques
    22.
    发明申请
    Microfluidic protein crystallography techniques 有权
    微流体蛋白晶体学技术

    公开(公告)号：US20050062196A1

    公开(公告)日：2005-03-24

    申请号：US10810350

    申请日：2004-03-26

    Applicant: Carl Hansen ,  Stephen Quake ,  James Berger

    Inventor： Carl Hansen ,  Stephen Quake ,  James Berger

    IPC: B29C33/40 ,  C12Q20060101

    CPC classification number: C30B29/16 ,  B01F5/10 ,  B01F5/108 ,  B01F13/0059 ,  B01L3/06 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  C30B7/00 ,  C30B7/14 ,  C30B29/58

    Abstract: The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.

    Abstract translation: 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另一个实施方案，所述孔可以被配置为容纳诸如冷冻保护剂的材料，其可用于在形成后保护结晶材料。

    Improved Methods for Rapid Gene Synthesis
    25.
    发明申请
    Improved Methods for Rapid Gene Synthesis 审中-公开
    改进的快速基因合成方法

    公开(公告)号：US20120156731A1

    公开(公告)日：2012-06-21

    申请号：US12052156

    申请日：2008-03-20

    Applicant: Yanyi Huang ,  James Berger ,  Stephen Quake

    Inventor： Yanyi Huang ,  James Berger ,  Stephen Quake

    IPC: C12P19/34 ,  C07H21/04 ,  C12M1/00 ,  C12N9/00

    CPC classification number: C12Y605/01001 ,  C12P19/34 ,  C12Y605/01002

    Abstract: Disclosed are methods and materials for assembling long polynucleotides from synthetic oligonucleotides. The use of synthetic oligonucleotides permits non-natural design of sequences. The oligonucleotides used for construction may be relatively short, according to practicalities of nucleotide synthesis. They are assembled using a ligase which is operative over a range of temperatures, i.e., is thermostable. The method and oligonucleotides are designed such that the melting temperature of the strands to be hybridized is set at a number of selected specific temperatures for each group of oligonucleotides to be hybridized and ligated. Hybridization and ligation take place at or near the melting temperature, so that each succeeding ligation is governed by a temperature that will prevent hybridization if any mismatches are present.

    Abstract translation: 公开了用于从合成寡核苷酸组装长多核苷酸的方法和材料。 合成寡核苷酸的使用允许序列的非自然设计。 根据核苷酸合成的实用性，用于构建的寡核苷酸可能相对较短。 它们使用在一定温度范围内操作的连接酶组装，即是耐热的。 设计该方法和寡核苷酸使待杂交的链的解链温度被设定为要混合并连接的每组寡核苷酸的选定的特定温度的数目。 杂交和连接在熔融温度或接近熔化温度下进行，因此每次后续连接都由温度控制，如果存在任何错配，将会阻止杂交。

    Determination of fetal aneuploidies by massively parallel DNA sequencing
    26.
    发明授权
    Determination of fetal aneuploidies by massively parallel DNA sequencing 有权
    通过大规模并行DNA测序确定胎儿非整倍体

    公开(公告)号：US08008018B2

    公开(公告)日：2011-08-30

    申请号：US12393803

    申请日：2009-02-26

    Applicant: Stephen Quake ,  Hei-Mun Christina Fan

    Inventor： Stephen Quake ,  Hei-Mun Christina Fan

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2537/157 ,  C12Q2527/125 ,  C12Q2545/114 ,  C12Q2527/137

    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.

    Abstract translation: 本方法的例子是一种方法，其中将含有胎儿DNA的母体血液稀释成每反应样品约0.5个基因组当量DNA的标称值。 然后使用数字PCR来检测非整倍体，例如导致唐氏综合征的三体性。 由于非整倍体并不表现出序列变异，仅仅是染色体数量的变化，所以不可能在胎儿中检测它们，而不需要采用诸如羊膜穿刺或绒毛膜绒毛取样等侵入性技术。 数字扩增允许使用大规模并行扩增和检测方法检测非整倍体，检查例如10,000个基因组等同物。

    Non-Invasive Fetal Genetic Screening by Digital Analysis
    27.
    发明申请
    Non-Invasive Fetal Genetic Screening by Digital Analysis 审中-公开

    公开(公告)号：US20100255492A1

    公开(公告)日：2010-10-07

    申请号：US12815674

    申请日：2010-06-15

    Applicant: Stephen Quake ,  Hei-Mun Christina Fan

    Inventor： Stephen Quake ,  Hei-Mun Christina Fan

    IPC: C12Q1/68

    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.

    Non-Invasive Fetal Genetic Screening by Digital Analysis
    30.
    发明申请
    Non-Invasive Fetal Genetic Screening by Digital Analysis 审中-公开

    公开(公告)号：US20090170114A1

    公开(公告)日：2009-07-02

    申请号：US12393833

    申请日：2009-02-26

    Applicant: Stephen Quake ,  Hei-Mun Christina Fan

    Inventor： Stephen Quake ,  Hei-Mun Christina Fan

    IPC: C12Q1/68

    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.

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