Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
    25.
    发明授权
    Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes 失效
    改性淀粉代谢酶和编码基因,用于改良和优化植物表型

    公开(公告)号:US06703240B1

    公开(公告)日:2004-03-09

    申请号:US09547844

    申请日:2000-04-12

    IPC分类号: C12N1500

    CPC分类号: C08L3/02

    摘要: The invention provides methods for generating, identifying, and selecting polynucleotides encoding novel starch metabolizing enzymes (NSME), NSME-encoding polynucleotides, compositions of recombinant shuffled NSME protein, plant cells and microbes containing a shuffled NSME polynucleotide in expressible form, plants containing a shuffled NSME polynucleotide in expressible form, novel starch compositions produced by plants and cells, uses of such plants, cells, and starch compositions.

    摘要翻译: 本发明提供了用于产生,鉴定和选择编码新型淀粉代谢酶(NSME),NSME编码多核苷酸,重组洗牌NSME蛋白质的组合物,植物细胞和含有可表达形式的洗牌NSME多核苷酸的微生物的多核苷酸的方法,所述植物含有洗牌 可表达形式的NSME多核苷酸,由植物和细胞产生的新型淀粉组合物,这种植物,细胞和淀粉组合物的用途。

    High throughput mass spectrometry
    26.
    发明授权
    High throughput mass spectrometry 有权
    高通量质谱

    公开(公告)号:US07384387B1

    公开(公告)日:2008-06-10

    申请号:US09502283

    申请日:2000-02-11

    IPC分类号: C40B30/10

    CPC分类号: C12Q1/04 G01N33/5005

    摘要: Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.

    摘要翻译: 提供了用于高通量质谱的装置和方法。 该方法涉及离线并行纯化系统中的样品制备。 这样的方法包括但不限于在产生样品时使用适当的缓冲液或者使用固体支持物用于标记的组分。 以这种方式制备的样品不需要进行柱分离。 提供的装置包括用于生长细胞并产生产物和/或反应物的细胞生长板,离线平行纯化系统,质谱仪和自动采样器,其将样品输送并注入到装置的质谱仪中。 所述的方法和装置例如用于筛选酶反应途径。