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1.发明申请Engineered imine reductases and methods for the reductive animation of ketone and amine compounds. 有权酮和胺化合物的还原动力的工程亚胺还原酶和方法。公开(公告)号：US20130302859A1
公开(公告)日：2013-11-14
申请号：US13890944
申请日：2013-05-09
申请人： CHEN Haibin , Steven J. Collier , Jovana NAZOR , Joly SUKUMARAN , Derek SMITH , Jeffrey C. MOORE , Gregory HUGHES , Jacob JANEY , Gjalt HUISMAN , Scott NOVICK , Nicholas AGARD , Oscar ALVIZO , Gregory COPE , Wan Lin YEO , Stephanie NG
发明人： CHEN Haibin , Steven J. Collier , Jovana NAZOR , Joly SUKUMARAN , Derek SMITH , Jeffrey C. MOORE , Gregory HUGHES , Jacob JANEY , Gjalt HUISMAN , Scott NOVICK , Nicholas AGARD , Oscar ALVIZO , Gregory COPE , Wan Lin YEO , Stephanie NG
IPC分类号： C12N9/00
CPC分类号： C12P13/02 , C12N9/0028 , C12P13/001 , C12P13/04 , C12P13/06 , C12P17/10 , C12P17/12 , C12P17/165 , C12P17/185 , C12P17/188 , C12Y105/01 , C12Y105/01023 , C12Y105/01024 , C12Y105/01028 , Y02P20/52
摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
摘要翻译： 本公开提供了具有亚胺还原酶活性的工程改造的多肽，编码工程改造的亚胺还原酶的多核苷酸，能够表达工程化亚胺还原酶的宿主细胞，以及使用这些工程改造的多肽与一系列酮和胺底物化合物以制备仲胺和叔胺的方法 产品化合物。
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2.发明授权Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes 失效改性淀粉代谢酶和编码基因，用于改良和优化植物表型公开(公告)号：US06703240B1
公开(公告)日：2004-03-09
申请号：US09547844
申请日：2000-04-12
IPC分类号： C12N1500
CPC分类号： C08L3/02
摘要： The invention provides methods for generating, identifying, and selecting polynucleotides encoding novel starch metabolizing enzymes (NSME), NSME-encoding polynucleotides, compositions of recombinant shuffled NSME protein, plant cells and microbes containing a shuffled NSME polynucleotide in expressible form, plants containing a shuffled NSME polynucleotide in expressible form, novel starch compositions produced by plants and cells, uses of such plants, cells, and starch compositions.
摘要翻译： 本发明提供了用于产生，鉴定和选择编码新型淀粉代谢酶（NSME），NSME编码多核苷酸，重组洗牌NSME蛋白质的组合物，植物细胞和含有可表达形式的洗牌NSME多核苷酸的微生物的多核苷酸的方法，所述植物含有洗牌 可表达形式的NSME多核苷酸，由植物和细胞产生的新型淀粉组合物，这种植物，细胞和淀粉组合物的用途。
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公开(公告)号：US08329438B2
公开(公告)日：2012-12-11
申请号：US12646907
申请日：2009-12-23
申请人： Christopher Savile , Vesna Mitchell , Xiyun Zhang , Gjalt Huisman
发明人： Christopher Savile , Vesna Mitchell , Xiyun Zhang , Gjalt Huisman
IPC分类号： C12N9/02
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
摘要翻译： 本公开涉及具有改进性质的工程化烯酮还原酶多肽，编码工程改造多肽的多核苷酸，相关载体，宿主细胞和用于制备工程化烯酮还原酶多肽的方法。 本公开还提供了使用工程化烯酮还原酶多肽进行化学转化的方法。
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4.发明授权Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine 有权用于从3-芳基-3-酮基丙胺生产3-芳基-3-羟基丙胺的酮还原酶多肽公开(公告)号：US08288141B2
公开(公告)日：2012-10-16
申请号：US12549293
申请日：2009-08-27
CPC分类号： C12P17/00 , C12N9/0006 , C12P13/001 , C12Y101/01164 , C12Y101/01184
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。 优化工程化的酮还原酶多肽，用于催化N-甲基-3-酮-3-（2-噻吩基）-1-丙胺向（S）-N-甲基-3-羟基-3-（2-噻吩基） -1-丙胺。
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5.发明授权公开(公告)号：US07148054B2
公开(公告)日：2006-12-12
申请号：US10194686
申请日：2002-07-11
申请人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswatan Subramanian , Linda A. Castle , Claus M. Krebber , Steve Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswatan Subramanian , Linda A. Castle , Claus M. Krebber , Steve Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
CPC分类号： C12R1/465 , A01K2267/02 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/90 , C12P17/162 , C12Q1/6811 , C12Q1/6827
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
摘要翻译： 本发明提供了使用重组和选择/筛选整个细胞和生物体以获得所需性质的进化的迭代循环的方法。这些性质的实例包括增加的重组原型，基因组拷贝数和蛋白质和次级代谢物的表达和/或分泌能力 。
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公开(公告)号：US20050272064A1
公开(公告)日：2005-12-08
申请号：US11067323
申请日：2005-02-23
申请人： S. Davis , Richard Fox , Gjalt Huisman , Vesna Gavrilovic , Lisa Newman
发明人： S. Davis , Richard Fox , Gjalt Huisman , Vesna Gavrilovic , Lisa Newman
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
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7.发明授权Engineered imine reductases and methods for the reductive animation of ketone and amine compounds 有权酮和胺化合物的还原动力的工程亚胺还原酶和方法公开(公告)号：US09193957B2
公开(公告)日：2015-11-24
申请号：US13890944
申请日：2013-05-09
申请人： Haibin Chen , Steven J. Collier , Jovana Nazor , Joly Sukumaran , Derek Smith , Jeffrey C. Moore , Gregory Hughes , Jacob Janey , Gjalt Huisman , Scott Novick , Nicholas Agard , Oscar Alvizo , Gregory Cope , Wan Lin Yeo , Stefanie Ng Minor
发明人： Haibin Chen , Steven J. Collier , Jovana Nazor , Joly Sukumaran , Derek Smith , Jeffrey C. Moore , Gregory Hughes , Jacob Janey , Gjalt Huisman , Scott Novick , Nicholas Agard , Oscar Alvizo , Gregory Cope , Wan Lin Yeo , Stefanie Ng Minor
IPC分类号： C12N9/06 , C12P13/00 , C12P13/02 , C12P13/04 , C12P13/06 , C12P17/10 , C12P17/12 , C12P17/16 , C12P17/18
CPC分类号： C12P13/02 , C12N9/0028 , C12P13/001 , C12P13/04 , C12P13/06 , C12P17/10 , C12P17/12 , C12P17/165 , C12P17/185 , C12P17/188 , C12Y105/01 , C12Y105/01023 , C12Y105/01024 , C12Y105/01028 , Y02P20/52
摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
摘要翻译： 本公开提供了具有亚胺还原酶活性的工程改造的多肽，编码工程改造的亚胺还原酶的多核苷酸，能够表达工程化亚胺还原酶的宿主细胞，以及使用这些工程改造的多肽与一系列酮和胺底物化合物以制备仲胺和叔胺的方法 产品化合物。
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8.发明授权Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine 有权用于从3-芳基-3-酮基丙胺生产3-芳基-3-羟基丙胺的酮还原酶多肽公开(公告)号：US08426178B2
公开(公告)日：2013-04-23
申请号：US12549154
申请日：2009-08-27
CPC分类号： C12N9/0006 , C12P13/001 , C12P17/00 , C12P41/002
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2- thienyl)-1-propanamine.
摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。 优化了工程化酮还原酶多肽，用于催化N，N-二甲基-3-酮-3-（2-噻吩基）-1-酮基丙胺转化为（S）-N，N-二甲基-3-羟基-3-（ 2-噻吩基）-1-丙胺。
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9.发明申请公开(公告)号：US20120252681A1
公开(公告)日：2012-10-04
申请号：US13310332
申请日：2011-12-02
申请人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C40B10/00
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。
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10.发明授权公开(公告)号：US07977078B2
公开(公告)日：2011-07-12
申请号：US12197286
申请日：2008-08-24
申请人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Rama Voladri , James Lalonde , Gjalt Huisman
发明人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Rama Voladri , James Lalonde , Gjalt Huisman
CPC分类号： C12P17/00 , C12N9/0006 , C12P17/167 , C12Y101/01184 , Y02P20/52
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成手性化合物的方法。
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