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1103 条记录 (耗时 0.047 秒)

    Feature intensity reconstruction of biological probe array
    21.
    发明授权
    Feature intensity reconstruction of biological probe array 有权
    生物探针阵列特征强度重建

    公开(公告)号：US08934689B2

    公开(公告)日：2015-01-13

    申请号：US13663673

    申请日：2012-10-30

    申请人： Affymetrix, Inc.

    发明人： Earl A. Hubbell

    IPC分类号： G06K9/00 ,  G06F19/20

    CPC分类号： G06K9/0014 ,  G06F19/20 ,  G06K9/00 ,  G06K2209/07

    摘要： The invention provides methods and systems for reconstructing feature intensities from pixel level data. In certain embodiments, the invention uses an empirically determined transfer function to construct a theoretical estimate of pixel level data and then iteratively updates feature intensities based on a minimum multiplicative error between the pixel level data and the theoretical estimate of the pixel level data.

    摘要翻译： 本发明提供了用于从像素级数据重建特征强度的方法和系统。 在某些实施例中，本发明使用经验确定的传递函数来构建像素级数据的理论估计，然后基于像素级数据与像素级数据的理论估计之间的最小乘法误差迭代地更新特征强度。

    Assays for affinity profiling of nucleic acid binding proteins
    22.
    发明授权
    Assays for affinity profiling of nucleic acid binding proteins 有权
    核酸结合蛋白亲和性分析的方法

    公开(公告)号：US08932819B2

    公开(公告)日：2015-01-13

    申请号：US13543711

    申请日：2012-07-06

    申请人： Philip Dickinson

    发明人： Philip Dickinson

    IPC分类号： G01N33/53 ,  C12Q1/68

    CPC分类号： G01N33/5308 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2522/101 ,  C12Q2533/101 ,  C12Q2565/525

    摘要： Methods, compositions and kits are disclosed for assays to determine the binding affinity of DNA-binding proteins or RNA-binding proteins for their corresponding recognition site(s). In particular, assays are disclosed for measuring binding affinities when either the binding protein, or the recognition sequence of the recognition site, or cofactor proteins, contain one or more mutations. The disclosed assays can thus be utilized to measure the effect on transcription factor binding caused by mutations within the recognition site, or mutations within the binding domain of the protein, and to provide binding affinity information that can be correlated with altered gene regulation and expression. The disclosed assays can be personalized to a specific person or organism, with the measured binding affinities based upon an individual's specific binding proteins and recognition sites. Furthermore, embodiments are capable of measuring binding affinities between multiple binding proteins and multiple recognition sites through an entirely in vitro process.

    摘要翻译： 公开了用于测定DNA结合蛋白或RNA结合蛋白对于其相应识别位点的结合亲和力的方法，组合物和试剂盒。 特别地，公开了当结合蛋白或识别位点的识别序列或辅因子蛋白含有一个或多个突变时测量结合亲和力的测定。 因此，所公开的测定可用于测量由识别位点内的突变或蛋白质结合结构域内的突变引起的对转录因子结合的影响，并提供可与改变的基因调节和表达相关的结合亲和力信息。 所公开的测定可以针对特定人或生物进行个性化，其中所测量的结合亲和力基于个体的特异性结合蛋白和识别位点。 此外，实施方案能够通过完全体外方法测量多个结合蛋白和多个识别位点之间的结合亲和力。

    Methods for genotyping selected polymorphism
    24.
    发明授权
    Methods for genotyping selected polymorphism 有权
    选择多态性基因分型的方法

    公开(公告)号：US08808995B2

    公开(公告)日：2014-08-19

    申请号：US13348394

    申请日：2012-01-11

    申请人： Ketih W. Jones ,  Michael H. Shapero ,  Stephen P. A. Fodor

    发明人： Ketih W. Jones ,  Michael H. Shapero ,  Stephen P. A. Fodor

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6855 ,  C12Q2600/156

    摘要： Methods for genotyping polymorphisms using a locus specific primer that is complementary to a region near a selected polymorphism are described. Methods for synthesizing pools of locus specific primers that incorporate some degenerate positions are also disclosed. A plurality of different sequence capture probes are synthesized simultaneously using degenerate oligonucleotide synthesis. The sequence of the locus specific regions of the capture probes are related in that they have some bases that are identical in each sequence in the plurality of sequences and positions that vary from one locus specific region to another. The sequences are selected based on proximity to a polymorphism of interest and because they conform to a similar sequence pattern.

    摘要翻译： 描述使用与选定多态性附近的区域互补的位点特异性引物进行基因分型多态性的方法。 还公开了合并含有一些退化位置的基因座特异性引物库的方法。 使用简并寡核苷酸合成同时合成多个不同的序列捕获探针。 捕获探针的基因座特异性区域的序列是相关的，因为它们具有在从一个基因座特异性区域到另一个基因座特异性区域变化的多个序列和位置中的每个序列中具有相同的一些碱基。 基于与感兴趣的多态性的接近度并且因为它们符合类似的序列模式来选择序列。

    Miniaturized microparticles
    26.
    发明申请
    Miniaturized microparticles 有权
    微型微粒

    公开(公告)号：US20140057809A1

    公开(公告)日：2014-02-27

    申请号：US13760849

    申请日：2013-02-06

    申请人： AFFYMETRIX, INC.

    发明人： Randall J. True

    IPC分类号： G01N33/532

    CPC分类号： G01N33/532 ,  B82Y25/00 ,  G01N33/585 ,  G03F7/0002 ,  G03F7/0035 ,  G09F3/00 ,  H01F1/0072 ,  Y10T428/2989 ,  Y10T428/2996 ,  Y10T436/143333

    摘要： A system and method for forming encoded microparticles is described. One embodiment includes an encoded microparticle, the microparticle comprising a plurality of segments aligned along an axis, wherein the plurality of segments define a code for the microparticle; and an outer cuboid encapsulating the plurality of segments, wherein the plurality of segments are detectable through the outer cuboid.

    摘要翻译： 描述了用于形成编码的微粒的系统和方法。 一个实施例包括编码的微粒，所述微粒包括沿着轴对准的多个区段，其中所述多个区段限定所述微粒的代码; 以及封装所述多个段的外部长方体，其中所述多个段可通过所述外部长方体检测。

    Recombinant Colwellia Psychrerythraea Alkaline Phosphatase and Uses Thereof
    28.
    发明申请
    Recombinant Colwellia Psychrerythraea Alkaline Phosphatase and Uses Thereof 审中-公开
    重组Colwellia Psychrerythraea碱性磷酸酶及其用途

    公开(公告)号：US20130344493A1

    公开(公告)日：2013-12-26

    申请号：US13916932

    申请日：2013-06-13

    申请人： Affymetrix, Inc.

    发明人： Jeannine C. Muller-Greven ,  Marc A. Post ,  Christopher J. Kubu

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  C12Y301/03001

    摘要： A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated from Colwellia psychrerythraea are provided. Methods of over-expression and purification of the recombinant alkaline phosphatase and mutants thereof are also disclosed. Methods of over-expressing and purifying commercially useful quantities of active recombinant heat labile alkaline phosphatase fusion enzymes from C. psychrerythraea, wherein the fusion enzymes comprise one or more heterologous leader sequences are disclosed. The disclosed C. psychrerythraea heat labile alkaline phosphatase has properties similar to shrimp alkaline phosphatase and can be substituted for shrimp alkaline phosphatase in assays involving the same.

    摘要翻译： 公开了一种热不稳定碱性磷酸酶及其使用方法及其试剂盒。 具体地，提供了从Colleyia psychrerythraea分离的热不稳定碱性磷酸酶的肽序列，使用方法和试剂盒的核苷酸序列。 还公开了重组碱性磷酸酶及其突变体的过表达和纯化的方法。 过度表达和纯化商业上有用量的来自苦参精油的活性重组热不稳定碱性磷酸酶融合酶的方法，其中所述融合酶包含一个或多个异源前导序列。 所披露的C. psychrerythraea热不稳定碱性磷酸酶具有类似于虾碱性磷酸酶的性质，并且可以在涉及其的测定中替代虾碱性磷酸酶。