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公开(公告)号：US20030068612A1
公开(公告)日：2003-04-10
申请号：US10180235
申请日：2002-06-26
Inventor： Marc Vidal , Jef D. Boeke , Ed Harlow
IPC: C12Q001/68 , C12N001/18
CPC classification number: G01N33/5023 , C12N15/1055 , C12Q1/6897 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/505 , G01N33/53 , G01N2333/39 , C12Q2565/201 , C12Q2522/101
Abstract: Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.
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公开(公告)号：US20020197627A1
公开(公告)日：2002-12-26
申请号：US10107786
申请日：2002-03-27
Inventor： Marc Vidal , Jef D. Boeke , Ed Harlow
IPC: C12Q001/68 , C12N001/18 , C12N015/74
CPC classification number: G01N33/5023 , C12N15/1055 , C12Q1/6897 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/505 , G01N33/53 , G01N2333/39 , C12Q2565/201 , C12Q2522/101
Abstract: Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.
Abstract translation: 公开了用于鉴定分子相互作用（例如蛋白质/蛋白质，蛋白质/ DNA，蛋白质/ RNA或RNA / RNA相互作用）的方法。 本发明中的所有方法都采用反选择和至少两种杂交分子。 相互作用的分子重构转录因子并直接表达报告基因，然后测定其表达。 还公开了可用于实践本发明的方法的遗传构建体。
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公开(公告)号：US20020106773A1
公开(公告)日：2002-08-08
申请号：US09973064
申请日：2001-10-10
Applicant: Myriad Genetics, Inc.
Inventor： Jean-Marc Roch , Paul L. Bartel , Karen Heichman
IPC: C12N009/16 , C12Q001/68 , G01N033/53 , C07K016/40
CPC classification number: G01N33/6896 , A61K38/1709 , C07K14/47 , C07K14/4711 , C07K16/18 , C07K16/2833 , C07K2317/32 , C12Q1/6883 , C12Q1/6897 , C12Q2600/156 , G01N2500/00 , G01N2500/02 , G01N2800/28 , C12Q2565/201
Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.
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公开(公告)号：US07422856B2
公开(公告)日：2008-09-09
申请号：US11447182
申请日：2006-06-05
Applicant: Thomas C. Südhof , Thomas Biederer , Angela Ho , Xinran Liu
Inventor： Thomas C. Südhof , Thomas Biederer , Angela Ho , Xinran Liu
CPC classification number: G01N33/5008 , A01K2217/05 , A01K2217/075 , C07K14/4702 , C07K14/4711 , C07K14/70567 , C07K2319/00 , C12Q1/6897 , G01N33/5023 , G01N33/5035 , G01N33/6896 , G01N2333/4709 , G01N2500/20 , G01N2800/2821 , C12Q2565/201
Abstract: The present invention is directed to isolated nucleic acids encoding Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of the amyloid precursor protein (APP) relative to wild-type Mint proteins. The present invention is further directed toward purified Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of APP relative to wild-type Mint proteins. The present invention also encompasses methods of modulating transcriptional activation and methods of identifying compounds that modulate transcriptional activation, and vectors, as well as transfected cells and kits useful for modulating transcriptional activation or for the identification of compounds that can modulate transcriptional activation. The present invention further encompasses transgenic knockout mice with little or no expression of Mint 1, Mint 2 or Mint 3 proteins. Such reagents may be useful as candidate therapeutics for Alzheimer's disease (AD), or as models for the rational design of drugs useful for the treatment of AD.
Abstract translation: 本发明涉及编码具有增强的相对于野生型薄荷蛋白调节由淀粉样蛋白前体蛋白（APP）的细胞质尾部介导的转录激活的能力的Mint蛋白变体的分离的核酸。 本发明还涉及具有相对于野生型薄荷蛋白调节由APP的细胞质尾巴介导的转录激活的能力的纯化的薄荷蛋白变体。 本发明还包括调节转录激活的方法和鉴定调节转录激活的化合物的方法，以及载体，以及用于调节转录激活或用于鉴定可调节转录激活的化合物的转染细胞和试剂盒。 本发明还包括具有很少或没有表达Mint1，Mint2或Mint3蛋白的转基因敲除小鼠。 这些试剂可用作阿尔茨海默病（AD）的候选治疗剂，或作为用于治疗AD的药物的合理设计的模型。
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公开(公告)号：US07393318B2
公开(公告)日：2008-07-01
申请号：US10801994
申请日：2004-03-16
Applicant: J. Keith Joung , Jeffrey Miller , Carl O. Pabo
Inventor： J. Keith Joung , Jeffrey Miller , Carl O. Pabo
CPC classification number: G01N33/502 , C12N15/1055 , C12Q1/6897 , G01N33/50 , G01N33/5008 , C12Q2565/201
Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.
Abstract translation: 本发明提供了用于检测蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的相互作用阱测定法的方法和组合物。 本发明的方法和组合物还可用于鉴定可能激动或拮抗蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的试剂。 在某些实施方案中，本发明的相互作用捕获系统可用于筛选具有大于10个成员的文库。 在其它实施方案中，本发明的相互作用阱系统结合流式细胞术使用。 本发明还提供了用于同时筛选靶蛋白或核酸序列以获得与两种或多种测试蛋白或核酸相互作用的能力的方法。
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Patent Agency Ranking
公开(公告)号：US07297491B2
公开(公告)日：2007-11-20
申请号：US10915233
申请日：2004-08-10
Applicant: J. Keith Joung , Jeffrey Miller , Carl O. Pabo
Inventor： J. Keith Joung , Jeffrey Miller , Carl O. Pabo
IPC: C12Q1/68
CPC classification number: C12N15/1055 , C12Q1/6897 , G01N33/50 , G01N33/5008 , G01N33/502 , C12Q2565/201
Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interactions. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.
Abstract translation: 本发明提供了用于检测蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的相互作用阱测定法的方法和组合物。 本发明的方法和组合物还可用于鉴定可能激动或拮抗蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的试剂。 在某些实施方案中，本发明的相互作用捕获系统可用于筛选具有大于10个成员的文库。 在其它实施方案中，本发明的相互作用阱系统结合流式细胞术使用。 本发明还提供了用于同时筛选靶蛋白或核酸序列以获得与两种或多种测试蛋白或核酸相互作用的能力的方法。
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公开(公告)号：US20070037188A1
公开(公告)日：2007-02-15
申请号：US11447182
申请日：2006-06-05
Applicant: Thomas Sudhof , Thomas Biederer , Angela Ho , Xinran Liu
Inventor： Thomas Sudhof , Thomas Biederer , Angela Ho , Xinran Liu
IPC: C12Q1/68 , C07H21/04 , C12P21/06 , C07K14/705
CPC classification number: G01N33/5008 , A01K2217/05 , A01K2217/075 , C07K14/4702 , C07K14/4711 , C07K14/70567 , C07K2319/00 , C12Q1/6897 , G01N33/5023 , G01N33/5035 , G01N33/6896 , G01N2333/4709 , G01N2500/20 , G01N2800/2821 , C12Q2565/201
Abstract: The present invention is directed to isolated nucleic acids encoding Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of the amyloid precursor protein (APP) relative to wild-type Mint proteins. The present invention is further directed toward purified Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of APP relative to wild-type Mint proteins. The present invention also encompasses methods of modulating transcriptional activation and methods of identifying compounds that modulate transcriptional activation, and vectors, as well as transfected cells and kits useful for modulating transcriptional activation or for the identification of compounds that can modulate transcriptional activation. The present invention further encompasses transgenic knockout mice with little or no expression of Mint 1, Mint 2 or Mint 3 proteins. Such reagents may be useful as candidate therapeutics for Alzheimer's disease (AD), or as models for the rational design of drugs useful for the treatment of AD.
Abstract translation: 本发明涉及编码具有增强的相对于野生型薄荷蛋白调节由淀粉样蛋白前体蛋白（APP）的细胞质尾部介导的转录激活的能力的Mint蛋白变体的分离的核酸。 本发明还涉及具有相对于野生型薄荷蛋白调节由APP的细胞质尾巴介导的转录激活的能力的纯化的薄荷蛋白变体。 本发明还包括调节转录激活的方法和鉴定调节转录激活的化合物的方法，以及载体，以及用于调节转录激活或用于鉴定可调节转录激活的化合物的转染细胞和试剂盒。 本发明还包括具有很少或没有表达Mint1，Mint2或Mint3蛋白的转基因敲除小鼠。 这些试剂可用作阿尔茨海默病（AD）的候选治疗剂，或作为用于治疗AD的药物的合理设计的模型。
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公开(公告)号：US07045298B2
公开(公告)日：2006-05-16
申请号：US10109886
申请日：2002-04-01
Applicant: Tomoyasu Taniguchi , Junko Mizukami
Inventor： Tomoyasu Taniguchi , Junko Mizukami
IPC: G01N33/53 , G01N33/567
CPC classification number: G01N33/566 , C12Q1/6897 , G01N2333/70567 , G01N2500/02 , C12Q2565/201
Abstract: A method for identifying or screening an agonist for or antagonist to a peroxisome proliferator activated receptor (PPAR) which comprises allowing a test cell and a substance to be tested to coexist, and detecting a change in a ligand-dependent interaction between the PPAR and a coactivator in the test cells due to the substance to be tested by measuring the expression of a reporter gene as an index.
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公开(公告)号：US07033768B2
公开(公告)日：2006-04-25
申请号：US10027219
申请日：2001-12-21
Applicant: Marc Vidal , Jef D. Boeke , Ed Harlow
Inventor： Marc Vidal , Jef D. Boeke , Ed Harlow
IPC: G01N33/53
CPC classification number: G01N33/5023 , C12N15/1055 , C12Q1/6897 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/505 , G01N33/53 , G01N2333/39 , C12Q2565/201 , C12Q2522/101
Abstract: Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.
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30.发明申请Assembly and screening of highly complex and fully human antibody repertoire in yeast 审中-公开组装和筛选酵母中高度复杂和完全的人体抗体谱公开(公告)号：US20050123996A1
公开(公告)日：2005-06-09
申请号：US10947610
申请日：2004-09-21
Applicant: Li Zhu , Shaobing Hua , James Sheridan , Yu-Huei Lin
Inventor： Li Zhu , Shaobing Hua , James Sheridan , Yu-Huei Lin
IPC: C07K16/00 , C12N1/19 , C12Q1/68 , C40B30/04 , G01N33/68 , G01N33/53 , C12N1/18 , C12N15/74 , G01N33/543
CPC classification number: C40B30/04 , C07K16/00 , C07K2317/21 , C07K2317/55 , C12Q1/6897 , G01N33/6845 , C12Q2565/201
Abstract: Compositions, methods, and kits are provided for efficiently generating and screening a library of highly diverse protein complexes for their ability to bind to other proteins or oligonucleotide sequences. In one aspect of the invention, a library of expression vectors is provided for expressing the library of protein complexes. The library comprises a first nucleotide sequence encoding a first polypeptide subunit; and a second nucleotide sequence encoding a second polypeptide subunit. The first and second nucleotide sequences each independently vary within the library of expression vectors. In addition, the first and second polypeptide subunit are expressed as separate proteins which self-assemble to form a protein complex, such as a double-chain antibody fragment (dcFv or Fab) and a fully assembled antibody, in cells into which the library of expression vectors are introduced. The library of expression vectors can be efficiently generated in yeast cells through homologous recombination; and the encoded proteins complexes with high binding affinity to their target molecule can be selected by high throughput screening in vivo or in vitro.
Abstract translation: 提供了组合物，方法和试剂盒，用于有效地产生和筛选高度多样化的蛋白质复合物的文库以获得与其它蛋白质或寡核苷酸序列结合的能力。 在本发明的一个方面，提供表达载体文库用于表达蛋白质复合物的文库。 该文库包含编码第一多肽亚基的第一核苷酸序列; 和编码第二多肽亚基的第二核苷酸序列。 第一和第二核苷酸序列各自独立地在表达载体的文库内变化。 此外，第一和第二多肽亚基表达为分离的蛋白，其自组装形成蛋白复合物，例如双链抗体片段（dcFv或Fab）和完全组装的抗体，其中文库 引入表达载体。 表达载体文库可以通过同源重组在酵母细胞中有效产生; 并且可以通过体内或体外的高通量筛选来选择对其靶分子具有高结合亲和力的编码蛋白复合物。
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