公开(公告)号：US20210332352A1

公开(公告)日：2021-10-28

申请号：US17239538

申请日：2021-04-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Christian Siltanen ,  William Watterson ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/10 ,  C12N15/113 ,  C12M1/26 ,  C12M1/34

Abstract: This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US20210317402A1

公开(公告)日：2021-10-14

申请号：US17355295

申请日：2021-06-23

Applicant: Inscripta, Inc.

Inventor： Eric Abbate ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Richard Fox ,  Daniel Held ,  Michael Clay ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US20210317400A1

公开(公告)日：2021-10-14

申请号：US17355901

申请日：2021-06-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US11142740B2

公开(公告)日：2021-10-12

申请号：US16536049

申请日：2019-08-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  C12N15/00 ,  B01L3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20210310012A1

公开(公告)日：2021-10-07

申请号：US17353282

申请日：2021-06-21

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US11136572B2

公开(公告)日：2021-10-05

申请号：US17230241

申请日：2021-04-14

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20210254105A1

公开(公告)日：2021-08-19

申请号：US17173137

申请日：2021-02-10

Applicant: Inscripta, Inc.

Inventor： Matthew Estes ,  Chad Sanada ,  Miles Gander ,  Eileen Spindler

IPC: C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited yeast cells in a cell population when employing nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20210222159A1

公开(公告)日：2021-07-22

申请号：US17200443

申请日：2021-03-12

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Kevin Ness ,  Phillip Belgrader ,  Don Masquelier ,  Ryan Gill

IPC: C12N15/10 ,  C40B40/02 ,  C12M1/26 ,  C12M1/42 ,  C12M1/34 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36 ,  C12M1/00 ,  C12N9/22 ,  C12N15/11

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：US11066675B2

公开(公告)日：2021-07-20

申请号：US17140056

申请日：2021-01-02

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20210214671A1

公开(公告)日：2021-07-15

申请号：US16740420

申请日：2020-01-11

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Erik Zimmerman ,  Emily Feldman ,  Benjamin Mijts ,  Andrew Garst

IPC: C12M1/36 ,  C12N15/10

Abstract: The present disclosure provides compositions, automated multi-module instruments and methods to increase the percentage of edited mammalian cells in a cell population when employing nucleic-acid guided editing.