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公开(公告)号：US20120103098A1
公开(公告)日：2012-05-03
申请号：US12914370
申请日：2010-10-28
Applicant: James A. Laugharn, JR. , Carl Beckett
Inventor： James A. Laugharn, JR. , Carl Beckett
IPC: G01N29/28
CPC classification number: B01J19/10 , A61L2/025 , B01F3/08 , B01F3/12 , B01F11/02 , B01F11/0283 , B01F2215/0037 , B01J19/006 , B01J19/008 , B01J19/245 , B01J2219/00094 , C12N13/00
Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet/outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.
Abstract translation: 使用连续方法来声学处理材料的方法和系统，其中可以使材料以连续或间歇的方式流入/离开材料暴露于聚焦声能的声学处理室。 方法和系统可以被布置成允许在较长时间内连续处理，同时声能源以相对高的功率输出操作。 治疗室可以包括诸如声窗，热交换器，入口/出口流布置，检查窗，限定治疗体积大小或形状的插入元件等特征。与治疗室相对于治疗室的布置有关的治疗系统构型 提供声源和耦合介质，材料流动路径等。
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公开(公告)号：US07521023B2
公开(公告)日：2009-04-21
申请号：US10777014
申请日：2004-02-11
Applicant: James A. Laugharn, Jr. , Brevard S. Garrison
Inventor： James A. Laugharn, Jr. , Brevard S. Garrison
CPC classification number: B01F13/0277 , B01F11/02 , B01F11/0283 , B01F13/0059 , B01F13/0222 , B01F15/00175 , B01F15/00285 , B01F2215/0427 , B01F2215/0431 , B01F2215/045 , B01F2215/0454 , B01F2215/0468 , B01F2215/0477 , B01J2219/00317 , B01J2219/00486 , B01J2219/00659 , C12M35/04 , C40B60/14 , G01N2035/00554 , G01N2291/02836 , G01N2291/02872 , G01N2291/02881
Abstract: Apparatus and methods are disclosed for treating a sample by selectively controlling sonic energy and/or selectively controlling the location of the sample relative to the sonic energy.
Abstract translation: 公开了用于通过选择性地控制声能和/或选择性地控制样品相对于声能的位置来处理样品的装置和方法。
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公开(公告)号：US06274726B1
公开(公告)日：2001-08-14
申请号：US09016062
申请日：1998-01-30
Applicant: James A. Laugharn, Jr. , Robert A. Hess , Feng Tao
Inventor： James A. Laugharn, Jr. , Robert A. Hess , Feng Tao
IPC: C07H2100
CPC classification number: G01N30/00 , B01L3/5027 , B01L3/50273 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/14 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0688 , C12N1/06 , C12N15/101 , G01N27/44704 , G01N30/02 , G01N30/14 , G01N30/52 , G01N30/6047 , G01N2030/009 , G01N2030/285 , G01N2030/522 , G01N2030/8813 , B01D15/365 , B01D15/32
Abstract: The invention is based on the discovery that hyperbaric, hydrostatic pressure reversibly alters the partitioning of biomolecules between certain adsorbed and solvated phases relative to partitioning at ambient pressure. The new methods and devices disclosed herein make use of this discovery for highly selective and efficient, low salt isolation and purification of nucleic acids from a broad range of sample types, including forensic samples, blood and other body fluids, and cultured cells. In one embodiment, the invention features a pressure-modulation apparatus. The apparatus includes an electrode array system having at least two (i.e., two, three, four, or more) electrodes; and a conduit interconnecting the electrodes. The conduit contains an electrically conductive fluid in contact with a phase positioned in a pressure chamber. The phase can be, for example, a binding medium or stationary phase.
Abstract translation: 本发明基于以下发现：高压静水压力相对于在环境压力下的分配可逆地改变某些吸附和溶剂化相之间生物分子的分配。 本文公开的新方法和装置利用这一发现，从广泛范围的样品类型（包括法医样品，血液和其他体液以及培养的细胞）中高度选择性和有效的低盐分离和纯化核酸。在一个 本发明的特征在于压力调制装置。 该装置包括具有至少两个（即，两个，三个，四个或更多个）电极的电极阵列系统; 和连接电极的导管。 导管包含与定位在压力室中的相位接触的导电流体。 该相可以是例如结合介质或固定相。
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公开(公告)号：US06245506B1
公开(公告)日：2001-06-12
申请号：US08903615
申请日：1997-07-30
Applicant: James A. Laugharn, Jr. , Robert A. Hess
Inventor： James A. Laugharn, Jr. , Robert A. Hess
IPC: C12Q168
CPC classification number: C12Q1/37 , C12Q1/6874 , G01N33/6818 , G01N33/6848 , C12Q2527/109 , C12Q2537/149
Abstract: The invention is based on the discovery that the sequence of monomers in a polymeric biomolecule can be determined in a self-contained, high pressure reaction and detection apparatus, without the need for fluid flow into or out from the apparatus. The pressure is used to control the activity of enzymes that digest the polymeric biomolecule to yield the individual monomers in the sequence in which they existed in the polymer. High pressures modulate enzyme kinetics by reversibly inhibiting those enzymatic processes which result in a higher average activation volume, when compared to the ground state, and reversibly accelerating those processes which have lower activation volumes than the ground state. Modulating the pressure allows the experimenter to precisely control the activity of the enzyme. Conditions can be found, for example, where the enzyme removes only one monomer (e.g., a nucleotide or amino acid) from the biomolecule before the pressure is again raised to a prohibitive level. The identity of the single released nucleotide or amino acid can be determined using a detector that is in communication with a probe in the detection zone within the reaction vessel.
Abstract translation: 本发明基于以下发现：聚合物生物分子中的单体序列可以在独立的高压反应和检测装置中测定，而不需要流体流入或流出装置。 压力用于控制消化聚合物生物分子的酶的活性，以产生它们存在于聚合物中的序列中的各个单体。 高压通过可逆地抑制这些酶过程来调节酶动力学，其导致与基态相比更高的平均活化体积，并且可逆地加速具有比基态更低的活化体积的那些过程。 调节压力允许实验者精确控制酶的活性。 可以发现条件，例如，在再次将压力升至高水平之前酶仅从生物分子中除去一种单体（例如，核苷酸或氨基酸）的情况。 可以使用与反应容器内的检测区域中的探针连通的检测器来确定单个释放的核苷酸或氨基酸的身份。
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公开(公告)号：US6127534A
公开(公告)日：2000-10-03
申请号：US10892
申请日：1998-01-22
Applicant: Robert A. Hess , James A. Laugharn, Jr.
Inventor： Robert A. Hess , James A. Laugharn, Jr.
CPC classification number: C12N15/1006
Abstract: The invention is based on the discovery that pressure-induced changes in the free ion activity of a solution can be used to reversibly modulate the rate or the equilibrium position of chemical reactions, including catalytic reactions and associating/dissociating reactions. Pressure-induced changes in free-ion activity can also be used to improve separation processes.
Abstract translation: 本发明基于以下发现：溶液的自由离子活性的压力诱导变化可用于可逆地调节化学反应的速率或平衡位置，包括催化反应和缔合/解离反应。 压力诱导的游离离子活性变化也可用于改善分离过程。
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Patent Agency Ranking
公开(公告)号：US6111096A
公开(公告)日：2000-08-29
申请号：US962280
申请日：1997-10-31
Applicant: James A. Laugharn, Jr. , Robert A. Hess , Feng Tao
Inventor： James A. Laugharn, Jr. , Robert A. Hess , Feng Tao
IPC: B01L3/00 , C12M1/33 , C12N1/06 , C12N15/10 , G01N27/447 , G01N30/00 , G01N30/02 , G01N30/14 , G01N30/28 , G01N30/52 , G01N30/60 , G01N30/88 , C07H21/00
CPC classification number: G01N30/00 , B01L3/5027 , B01L3/50273 , C12N1/06 , C12N15/101 , G01N27/44704 , G01N30/52 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/14 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0688 , G01N2030/009 , G01N2030/285 , G01N2030/522 , G01N2030/8813 , G01N30/02 , G01N30/14 , G01N30/6047
Abstract: The invention is based on the discovery that hyperbaric, hydrostatic pressure reversibly alters the partitioning of nucleic acids between certain adsorbed and solvated phases relative to partitioning at ambient pressure. The new methods and devices disclosed herein make use of this discovery for highly selective and efficient, low salt isolation and purification of nucleic acids from a broad range of sample types, including forensic samples, blood and other body fluids, and cultured cells.
Abstract translation: 本发明基于以下发现：高压静水压力相对于在环境压力下的分配可逆地改变在某些吸附和溶剂化相之间的核酸分配。 本文公开的新方法和装置利用这一发现，用于从范围广泛的样品类型（包括法医样品，血液和其他体液以及培养细胞）中高度选择性和有效的低盐分离和纯化核酸。
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公开(公告)号：US09080167B2
公开(公告)日：2015-07-14
申请号：US13678755
申请日：2012-11-16
Applicant: James A. Laugharn, Jr. , Edwin Rudd , Guillaume Durin
Inventor： James A. Laugharn, Jr. , Edwin Rudd , Guillaume Durin
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2523/301 , C12Q2527/125
Abstract: Method and apparatus for processing paraffin embedded samples, e.g., to disassociate paraffin from tissue components and/or other biomolecules from the paraffin. The sample may be exposed to focused acoustic energy while held in a vessel containing a non-solvent, aqueous solution. Disassociated paraffin may be emulsified into the liquid or otherwise separated from the sample.
Abstract translation: 用于处理石蜡包埋样品的方法和装置，例如将石蜡与组织成分和/或其它生物分子从石蜡中分离出来。 样品可以暴露于聚焦的声能，同时保持在含有非溶剂水溶液的容器中。 脱离的链烷烃可以乳化到液体中或以其他方式与样品分离。
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38.发明授权Methods and apparatus for treating samples with acoustic energy to form particles and particulates 有权用声能处理样品以形成颗粒和颗粒的方法和装置公开(公告)号：US08702836B2
公开(公告)日：2014-04-22
申请号：US11986274
申请日：2007-11-20
Applicant: James A. Laugharn, Jr. , Edwin Rudd
Inventor： James A. Laugharn, Jr. , Edwin Rudd
CPC classification number: B01J2/18
Abstract: The invention relates to a method of forming solid particles from a sample, which includes the step of exposing the sample to a focused acoustic field having an acoustic wave variable, until the solid particles achieve a desired state of particularization. The acoustic wave variable may be selected based, at least in part, on the desired state of particularization. The sample may be exposed to the focused acoustic field through a medium.
Abstract translation: 本发明涉及从样品形成固体颗粒的方法，其包括将样品暴露于具有声波可变的聚焦声场的步骤，直到固体颗粒达到期望的特定化状态。 声波变量可以至少部分地基于期望的特定化状态来选择。 样品可以通过介质暴露于聚焦声场。
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39.发明申请Methods and apparatus for treating samples with acoustic energy to form particles and particulates 有权用声能处理样品以形成颗粒和颗粒的方法和装置公开(公告)号：US20120144905A1
公开(公告)日：2012-06-14
申请号：US11986274
申请日：2007-11-20
Applicant: James A. Laugharn, JR. , Edwin Rudd
Inventor： James A. Laugharn, JR. , Edwin Rudd
IPC: G01N15/06
CPC classification number: B01J2/18
Abstract: The invention relates to a method of forming solid particles from a sample, which includes the step of exposing the sample to a focused acoustic field having an acoustic wave variable, until the solid particles achieve a desired state of particularization. The acoustic wave variable may be selected based, at least in part, on the desired state of particularization. The sample may be exposed to the focused acoustic field through a medium.
Abstract translation: 本发明涉及从样品形成固体颗粒的方法，其包括将样品暴露于具有声波可变的聚焦声场的步骤，直到固体颗粒达到期望的特定化状态。 声波变量可以至少部分地基于期望的特定化状态来选择。 样品可以通过介质暴露于聚焦声场。
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40.发明申请METHODS AND APPARATUS FOR ACOUSTIC TREATMENT OF SAMPLES FOR HEATING AND COOLING 审中-公开用于加热和冷却样品的声处理方法和装置公开(公告)号：US20120024867A1
公开(公告)日：2012-02-02
申请号：US13193004
申请日：2011-07-28
Applicant: James A. Laugharn, JR.
Inventor： James A. Laugharn, JR.
Abstract: Methods and systems relate to enhancing heat transfer between a vessel wall and a sample or coupling medium during focused acoustic processing. The vessel containing the sample may include a heat exchanger on an inner surface and/or an outer surface of the vessel that can have any suitable shape or dimension that increases the surface area of the vessel wall. In some embodiments, heat exchanger features may disrupt a boundary layer of a liquid sample at the vessel wall during focused acoustic processing. Accordingly, the temperature of the liquid sample can be appropriately controlled. In some cases, heating and/or cooling of the liquid sample may be performed efficiently. In an embodiment, a liquid sample may be heated at a rate of at least about 25 degrees C. per ml per minute.
Abstract translation: 方法和系统涉及在聚焦声学处理期间增强血管壁和样品或耦合介质之间的热传递。 容纳样品的容器可以包括在容器的内表面和/或外表面上的热交换器，其可以具有增加容器壁的表面积的任何合适的形状或尺寸。 在一些实施例中，在聚焦声学处理期间，热交换器特征可能破坏液体样品在血管壁处的边界层。 因此，可以适当地控制液体试样的温度。 在一些情况下，可以有效地进行液体样品的加热和/或冷却。 在一个实施方案中，可以以每分钟至少约25摄氏度的速率加热液体样品。
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