利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

73 条记录 (耗时 0.053 秒)

    Methods and Compositions for Large-Scale Analysis of Nucleic Acids Using DNA Deletions
    35.
    发明申请
    Methods and Compositions for Large-Scale Analysis of Nucleic Acids Using DNA Deletions 有权
    使用DNA缺失大规模分析核酸的方法和组合

    公开(公告)号：US20080213771A1

    公开(公告)日：2008-09-04

    申请号：US11938096

    申请日：2007-11-09

    申请人： Radoje T. Drmanac

    发明人： Radoje T. Drmanac

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C40B50/06 ,  C40B50/14 ,  C40B50/18 ,  C40B40/08

    CPC分类号： C12N15/10 ,  C12N15/66

    摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are constructs that include pairs of target sequences which are separated by a known distance in the polynucleotide from which they are derived.

    摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是构建物，其包括在衍生自它们的多核苷酸中被分离已知距离的靶序列对。

    Three dimensional arrays for detection or quantification of nucleic acid species
    38.
    发明授权
    Three dimensional arrays for detection or quantification of nucleic acid species 失效
    用于检测或定量核酸物种的三维阵列

    公开(公告)号：US06383742B1

    公开(公告)日：2002-05-07

    申请号：US08912885

    申请日：1997-08-15

    申请人： Radoje T. Drmanac ,  Snezana Drmanac

    发明人： Radoje T. Drmanac ,  Snezana Drmanac

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2561/125 ,  C12Q2535/131 ,  C12Q2537/113 ,  C12Q2537/107 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/197

    摘要： The present invention provides a method for detecting a target nucleic acid species including the steps of providing an array of probes affixed to a substrate and a plurality of labeled probes wherein each labeled probe is selected to have a first nucleic acid sequence which is complementary to a first portion of a target nucleic acid and wherein the nucleic acid sequence of at least one probe affixed to the substrate is complementary to a second portion of the nucleic acid sequence of the target, the second portion being adjacent to the first portion; applying a target nucleic acid to the array under suitable conditions for hybridization of probe sequences to complementary sequences; introducing a labeled probe to the array; hybridizing a probe affixed to the substrate to the target nucleic acid; hybridizing the labeled probe to the target nucleic acid; affixing the labeled probe to an adjacently hybridized probe in the array; and detecting the labeled probe affixed to the probe in the array.

    摘要翻译： 本发明提供了一种用于检测靶核酸物种的方法，包括以下步骤：提供固定在底物上的探针阵列和多个标记的探针，其中每个标记的探针被选择为具有与第一个核酸序列互补的第一个核酸序列 靶核酸的第一部分，并且其中固定于所述底物的至少一个探针的核酸序列与所述靶的核酸序列的第二部分互补，所述第二部分与所述第一部分相邻; 在合适的条件下将靶核酸应用于探针序列与互补序列的杂交; 将标记的探针引入阵列; 将附着于底物的探针与目标核酸杂交; 将标记的探针与靶核酸杂交; 将标记的探针附着到阵列中的相邻杂交探针; 并且检测固定在阵列中的探针的标记探针。

    Computer-aided analysis system for sequencing by hybridization
    39.
    发明授权
    Computer-aided analysis system for sequencing by hybridization 失效
    用于通过杂交测序的计算机辅助分析系统

    公开(公告)号：US06316191B1

    公开(公告)日：2001-11-13

    申请号：US09205865

    申请日：1998-12-04

    申请人： Radoje T. Drmanac ,  Radomir B. Crkvenjakov

    发明人： Radoje T. Drmanac ,  Radomir B. Crkvenjakov

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  C12Q1/6874 ,  C12Q2600/156 ,  G06F19/22 ,  C12Q2565/518 ,  C12Q2565/50 ,  C12Q2545/114 ,  C12Q2537/165

    摘要： The conditions under which oligonucleotide probes hybridize preferentially with entirely complementary and homologous nucleic acid targets are described. Using these hybridization conditions, overlapping oligonucleotide probes associate with a target nucleic acid. Following washes, positive hybridization signals are used to assemble the sequence of a given nucleic acid fragment. Representative target nucleic acids are applied as dots. Up to 100,000 probes of the type (A,T,C,G)(A,T,C,G)N8(A,T,C,G) are used to determine sequence information by simultaneous hybridization with nucleic acid molecules bound to a filter. Additional hybridization conditions are provided that allow stringent hybridization of 6-10 nucleotide long oligomers which extends the utility of the invention. A computer process determines the information sequence of the target nucleic acid which can include targets with the complexity of mammalian genomes. Sequence generation can be obtained for a large complex mammalian genome in a single process.

    摘要翻译： 描述寡核苷酸探针优先与完全互补和同源的核酸靶物杂交的条件。 使用这些杂交条件，重叠的寡核苷酸探针与靶核酸结合。 洗涤后，使用阳性杂交信号来组装给定核酸片段的序列。 代表性靶核酸作为点应用。 使用多达100,000种类型（A，T，C，G）（A，T，C，G）N8（A，T，C，G）的探针来确定序列信息，与核酸分子结合 一个过滤器 提供额外的杂交条件，其允许6-10个核苷酸长的寡聚体的严格杂交，其延伸了本发明的效用。 计算机过程确定目标核酸的信息序列，其可以包括具有哺乳动物基因组复杂性的靶标。 可以在单个过程中为大型复合哺乳动物基因组获得序列产生。

    Method of sequencing genomes by hybridization of oligonucleotide probes
    40.
    发明授权
    Method of sequencing genomes by hybridization of oligonucleotide probes 失效

    公开(公告)号：US6018041A

    公开(公告)日：2000-01-25

    申请号：US902167

    申请日：1997-07-29

    申请人： Radoje T. Drmanac ,  Radomir B. Crkvenjakov

    发明人： Radoje T. Drmanac ,  Radomir B. Crkvenjakov

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C12Q1/6827 ,  Y10T436/143333

    摘要： The conditions under which oligonucleotides hybridize only with entirely homologous sequences are recognized. The sequence of a given DNA fragment is read by the hybridization and assembly of positively hybridizing probes through overlapping portions. By simultaneous hybridization of DNA molecules applied as dots and bound onto a filter, representing single-stranded phage vector with the cloned insert, with about 50,000 to 100,000 groups of probes, the main type of which is (A,T,C,G)(A,T,C,G)N8(A,T,C,G), information for computer determination of a sequence of DNA having the complexity of a mammalian genome are obtained in one step. To obtain a maximally completed sequence, three libraries are cloned into the phage vector, M13, bacteriophage are used: with the 0.5 kb and 7 kbp insert consisting of two sequences, with the average distance in genomic DNA of 100 kbp. For a million bp of genomic DNA, 25,000 subclones of the 0.5 kbp are required as well as 700 subclones 7 kb long and 170 jumping subclones. Subclones of 0.5 kb are applied on a filter in groups of 20 each, so that the total number of samples is 2,120 per million bp. The process can be easily and entirely robotized for factory reading of complex genomic fragments or DNA molecules.