公开(公告)号：US10899792B2

公开(公告)日：2021-01-26

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02 ,  C12P21/00 ,  C07K1/14

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US20200339640A1

公开(公告)日：2020-10-29

申请号：US16929658

申请日：2020-07-15

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase

IPC: C07K14/435 ,  C12P21/02 ,  C12N15/70

Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.

公开(公告)号：US20200094446A1

公开(公告)日：2020-03-26

申请号：US16617635

申请日：2018-05-31

Applicant: Institute of National Colleges of Technology, Japan ,  Spiber Inc.

Inventor： Takaya SATO ,  Takashi MORINAGA ,  Ryo SATOH ,  Kentaro TAKAHASHI ,  Takehiro SATO

IPC: B29C39/00 ,  D01F4/02 ,  C08L89/00

Abstract: The present invention relates to a protein molded article including a protein, in which an ionic liquid is included inside the protein molded article. The present invention also relates to a production method of the protein molded article, including a step of adding the ionic liquid to a molding material of the protein molded article. Further, the present invention relates to a protein solution including a protein, an ionic liquid, and a solvent. Further, the present invention also relates to a protein molded article plasticizer including an ionic liquid.

公开(公告)号：US20200031886A1

公开(公告)日：2020-01-30

申请号：US16491633

申请日：2018-03-05

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Takashi Morinaga ,  Takehisa Maekawa

IPC: C07K14/435 ,  D01F4/02 ,  D02J1/22

Abstract: The present invention relates to a method for manufacturing a protein fiber, including an extension and contraction step of contracting or extending a protein raw fiber containing a protein by bringing the protein raw fiber into contact with a liquid or vapor; and a drying step of drying the protein raw fiber that has undergone the extension and contraction step while adjusting a length of the protein raw fiber to an arbitrary length.

公开(公告)号：US20200000091A1

公开(公告)日：2020-01-02

申请号：US16489003

申请日：2018-02-28

Applicant: Keio University ,  Spiber Inc.

Inventor： Kazuharu Arakawa ,  Masayuki Fujiwara ,  Nobuaki Kono ,  Yoshinori Ohta

IPC: A01N43/42 ,  A01N25/10 ,  A01N25/34 ,  C07K14/435 ,  D01F4/02 ,  D01F1/10

Abstract: One aspect of the present invention is a microbial growth inhibitor comprising xanthurenic acid.

公开(公告)号：US20190194403A1

公开(公告)日：2019-06-27

申请号：US16328986

申请日：2017-09-01

Applicant: Spiber Inc. ,  Tekunohama Co., Ltd.

Inventor： Shinji Hirai ,  Yukimasa Ubukata ,  Ayumi Abe ,  Koichi Kotaka ,  Yuki Nakayama ,  Junichi Noba

IPC: C08J5/00 ,  B29C43/02 ,  B29C43/00 ,  C07K14/435

CPC classification number: C08J5/00 ,  B29C43/003 ,  B29C43/02 ,  B29K2089/00 ,  C07K14/435 ,  C07K14/43518 ,  C08H1/00 ,  C08J2389/00 ,  C08L89/00

Abstract: The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.

公开(公告)号：US10329332B2

公开(公告)日：2019-06-25

申请号：US14437704

申请日：2013-12-17

Applicant: SPIBER INC.

Inventor： Kaori Sekiyama ,  Mizuki Ishikawa ,  Shinya Murata

IPC: C07K14/435 ,  C08J5/18 ,  C08L89/00 ,  B29C39/02 ,  B29C55/02 ,  B29C71/02 ,  A61K38/00 ,  B29L7/00

Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

公开(公告)号：US20180127553A1

公开(公告)日：2018-05-10

申请号：US15564373

申请日：2016-04-04

Applicant: SPIBER INC. ,  KOJIMA INDUSTRIES CORPORATION

Inventor： Kana ISHIDA ,  Hironori YAMAMOTO ,  Hiroaki SUZUMURA ,  Kazuhide SEKIYAMA

IPC: C08J3/09 ,  D01F1/10 ,  D01F4/02 ,  C08L89/00

CPC classification number: C08J3/097 ,  C07K1/14 ,  C07K14/00 ,  C07K14/435 ,  C08J2389/00 ,  C08L89/00 ,  C08L2203/16 ,  D01F1/10 ,  D01F4/02

Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at, least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same.

公开(公告)号：US09926348B2

公开(公告)日：2018-03-27

申请号：US15356818

申请日：2016-11-21

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase

IPC: C12P21/02 ,  C07K14/435 ,  C12N15/70 ,  C12N15/09

CPC classification number: C07K14/43518 ,  C12N15/09 ,  C12N15/70 ,  C12P21/02

Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the accumulation of an organic acid at the time of inducing the expression is reduced.

公开(公告)号：US09732125B2

公开(公告)日：2017-08-15

申请号：US14764472

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: C07K14/435 ,  A61K8/64 ,  A61K8/42 ,  A61K8/46 ,  A61Q19/00 ,  A61K8/02 ,  C08J3/12 ,  C08L89/00 ,  C09D7/12 ,  C08J3/05 ,  C08J3/07 ,  A61K47/42 ,  A61K9/51

CPC classification number: C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K9/5169 ,  A61K47/42 ,  A61K2800/412 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  C08J3/05 ,  C08J3/07 ,  C08J3/12 ,  C08J2389/00 ,  C08L89/00 ,  C09D7/65 ,  C09D7/68

Abstract: Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.