Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus
    31.
    发明授权
    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
    使用电渗析法调节盐浓度的装置,包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法

    公开(公告)号:US07722751B2

    公开(公告)日:2010-05-25

    申请号:US11440872

    申请日:2006-05-25

    IPC分类号: B01D61/48

    摘要: An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

    摘要翻译: 提供了一种调节盐浓度的装置,包括该盐浓度的实验室,以及使用该装置调节盐浓度的方法。 该装置包括:由阳离子交换膜和阴离子交换膜限定的反应室,其选自生物分子提取室,扩增室,杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中,可以通过调节电压的极性,强度和施加时间来原位调节盐浓度。

    Isolation and purification method of biomolecules using hydrogel
    32.
    发明授权
    Isolation and purification method of biomolecules using hydrogel 有权
    使用水凝胶分离和纯化生物分子的方法

    公开(公告)号:US07579151B2

    公开(公告)日:2009-08-25

    申请号:US11191221

    申请日:2005-07-27

    IPC分类号: C12Q1/68 C07H21/00

    CPC分类号: C12N15/1006

    摘要: Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.

    摘要翻译: 提供了使用水凝胶分离和纯化生物分子的方法,该方法包括:使含有带电生物分子的样品与水凝胶接触以将生物分子结合至水凝胶; 洗涤与生物分子结合的水凝胶; 并使用洗脱溶剂洗脱结合的生物分子。 根据该方法,使用具有大表面积的水凝胶将生物分子的隔离时间减少到5分钟以下,不需要诸如电磁体的外部装置,并且可以容易地实现小尺寸系统或LOC 通过聚合物图案化技术适用于微系统。

    METHOD OF TREATING SURFACE OF SUBSTRATE USED IN BIOLOGICAL REACTION SYSTEM
    33.
    发明申请
    METHOD OF TREATING SURFACE OF SUBSTRATE USED IN BIOLOGICAL REACTION SYSTEM 审中-公开
    处理生物反应体系中使用的底物表面的方法

    公开(公告)号:US20090018032A1

    公开(公告)日:2009-01-15

    申请号:US12238165

    申请日:2008-09-25

    IPC分类号: C40B40/06

    摘要: Provided is a method of treating a surface of a substrate used in a biochemical reaction system, the method including forming a polymer film on the surface by vapor deposition of a compound of formula (1) below and a compound of formula (2) below: (RO)3—Si—(CH2)n1—X  (1) (RO)3—Si—(CH2)n2—(CF2)m—X  (2) wherein R is one of a methyl group and an ethyl group, X is one of a methyl group and a trifluoromethyl group, n1 is an integer from 1 to 3, n2 is an integer from 1 to 10, and m is an integer from 1 to 10.

    摘要翻译: 本发明提供一种处理生物化学反应体系中使用的基板的表面的方法,该方法包括通过以下式(1)的化合物和下述式(2)的化合物的气相沉积在表面上形成聚合物膜: (RO)3-Si-(CH2)n1-X(1)<?in-line-formula description =“In-line-formula description =”In-line-formula“ (RO)3-Si-(CH2)n2-(CF2)mX(2)式(“R”) )<?in-line-formula description =“In-line formula”end =“tail”?>其中R是甲基和乙基之一,X是甲基和三氟甲基之一,n1是 1〜3的整数,n2为1〜10的整数,m为1〜10的整数。

    Microvalve device and apparatus adopting the same
    35.
    发明申请
    Microvalve device and apparatus adopting the same 审中-公开
    微阀装置及其采用的装置

    公开(公告)号:US20060169339A1

    公开(公告)日:2006-08-03

    申请号:US11346452

    申请日:2006-02-02

    IPC分类号: F15C1/04

    摘要: A microvalve device is provided. The microvalve device uses electrolysis and uses a hydrogel swelling and deswelling in response to anions or cations as an actuator for controlling the path of a flowing fluid. The microvalve device does not require a buffer solution but uses the transfer fluid flowing in a valve as a source driving the actuator. To generate the anions or cations, an electrode is needed for electrolysis of the fluid near the hydrogel. The microvalve is easy to manufacture and has a simple structure. In addition, the micro valve is useful to manufacture fluid channel arrays having various multi-channel structures.

    摘要翻译: 提供微型阀装置。 微阀装置使用电解,并且响应于阴离子或阳离子作为用于控制流动流体的路径的致动器,使用水凝胶溶胀和溶解。 微阀装置不需要缓冲溶液,而是使用流过阀的转移流体作为驱动致动器的源。 为了产生阴离子或阳离子,需要电极来电解水凝胶附近的流体。 微型阀门易于制造,结构简单。 此外,微阀可用于制造具有各种多通道结构的流体通道阵列。

    Handheld centrifuge
    40.
    发明授权
    Handheld centrifuge 有权
    手持离心机

    公开(公告)号:US07645223B2

    公开(公告)日:2010-01-12

    申请号:US11333857

    申请日:2006-01-17

    IPC分类号: B04B5/02 B04B9/00

    摘要: A handheld centrifuge includes an inertia body having an axle and a pair of inertia wheels coupled to the axle. The inertia wheels are coupled to the axle in substantially perpendicular direction to the axle, and spaced apart from each other. A string is connected to the axle. At least one closed vessel is detachably installed on an outer face of the inertia wheel to contain a substance to be centrifuged. The closed vessel is installed in substantially radial direction of the inertia wheel.

    摘要翻译: 手持式离心机包括惯性体,其具有轴和耦合到该轴的一对惯性轮。 惯性轮以基本上垂直于轴的方向联接到车轴并且彼此间隔开。 一根绳子连接到轴上。 至少一个密封容器可拆卸地安装在惯性轮的外表面上以容纳待离心的物质。 密闭容器沿惯性轮的大致径向安装。