摘要:
An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.
摘要:
Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
摘要:
Provided is a method of treating a surface of a substrate used in a biochemical reaction system, the method including forming a polymer film on the surface by vapor deposition of a compound of formula (1) below and a compound of formula (2) below: (RO)3—Si—(CH2)n1—X (1) (RO)3—Si—(CH2)n2—(CF2)m—X (2) wherein R is one of a methyl group and an ethyl group, X is one of a methyl group and a trifluoromethyl group, n1 is an integer from 1 to 3, n2 is an integer from 1 to 10, and m is an integer from 1 to 10.
摘要:
A method for real-time detection of polymerase chain reaction (PCR) is provided. The method includes: manufacturing a reactor having a plurality of electrodes; immobilizing a PCR primer to surfaces of electrodes; injecting a mixture for a PCR into the reactor to perform the PCR on the surfaces of electrodes and measuring an impedance of the PCR product.
摘要:
A microvalve device is provided. The microvalve device uses electrolysis and uses a hydrogel swelling and deswelling in response to anions or cations as an actuator for controlling the path of a flowing fluid. The microvalve device does not require a buffer solution but uses the transfer fluid flowing in a valve as a source driving the actuator. To generate the anions or cations, an electrode is needed for electrolysis of the fluid near the hydrogel. The microvalve is easy to manufacture and has a simple structure. In addition, the micro valve is useful to manufacture fluid channel arrays having various multi-channel structures.
摘要:
Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要:
Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
摘要:
Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要:
A transmembrane fusion protein including ubiquitin or a ubiquitin-like protein, a membrane translocation sequence linked to the C-terminus of the ubiquitin or ubiquitin-like protein, and a biologically active molecule linked to the C-terminus of the membrane translocation sequence is disclosed herein. A polynucleotide encoding the transmembrane fusion protein, a recombinant expression vector including the polynucleotide sequence, a cell transformed by the recombinant expression vector, and a method of delivering the biologically active molecule into a cell using the transmembrane fusion protein are also disclosed.
摘要:
A handheld centrifuge includes an inertia body having an axle and a pair of inertia wheels coupled to the axle. The inertia wheels are coupled to the axle in substantially perpendicular direction to the axle, and spaced apart from each other. A string is connected to the axle. At least one closed vessel is detachably installed on an outer face of the inertia wheel to contain a substance to be centrifuged. The closed vessel is installed in substantially radial direction of the inertia wheel.