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    Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
    35.
    发明申请
    Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast 有权
    甘油-3-磷酸O-酰基转移酶启动子用于油质酵母中的基因表达

    公开(公告)号：US20060057690A1

    公开(公告)日：2006-03-16

    申请号：US11225354

    申请日：2005-09-13

    Applicant: Zhixiong Xue ,  Quinn Zhu

    Inventor： Zhixiong Xue ,  Quinn Zhu

    IPC: C12P7/64 ,  C12N1/18 ,  C12N15/74

    CPC classification number: C12N15/815 ,  C12N9/1029 ,  C12P7/6427 ,  C12P21/02 ,  C12Y203/01015

    Abstract: The promoter region associated with the Yarrowia lipolytica glycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.

    Abstract translation: 已经发现与解脂耶氏酵母甘油-3-磷酸O-酰基转移酶（gpat）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已经显示本发明的启动子区域适合于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。

    Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts
    37.
    发明授权
    Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts 有权
    通过增加含油酵母中的Yap1转录因子活性来增加油含量

    公开(公告)号：US08865429B2

    公开(公告)日：2014-10-21

    申请号：US13339461

    申请日：2011-12-29

    Applicant: Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    Inventor： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC: C12P21/06 ,  C12N1/16 ,  C12P7/64 ,  C07K14/39

    CPC classification number: C07K14/39 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463

    Abstract: Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.

    Abstract translation: 本文描述了具有增加的油含量的转基因含油酵母，其包含增加的Yap1转录因子活性，其中增加的油含量与非转基因油质酵母的油含量进行比较。 通过增加转录因子与能够激活转录因子的蛋白质之间的相互作用，或通过其组合，Yap1转录因子活性的增加来自过表达Yap1转录因子。 还描述了使用这些酵母菌株的方法。

    Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    38.
    发明授权
    Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和含油生物体中的油含量

    公开(公告)号：US08685681B2

    公开(公告)日：2014-04-01

    申请号：US12338536

    申请日：2008-12-18

    Applicant: Narendra S. Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    Inventor： Narendra S. Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    IPC: C12P7/64 ,  C12N9/10 ,  C12P21/06 ,  C12N1/20 ,  C12N1/16 ,  C12N1/12 ,  C07H21/04

    CPC classification number: C12N9/1029 ,  C12P7/6463 ,  C12P7/6472

    Abstract: Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

    Abstract translation: 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
    39.
    发明授权
    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08637298B2

    公开(公告)日：2014-01-28

    申请号：US12814815

    申请日：2010-06-14

    Applicant: Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    Inventor： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC: C12N1/00

    CPC classification number: C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    Abstract: Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    Abstract translation: 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶 合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

    Delta-4 desaturase and its use in making polyunsaturated fatty acids
    40.
    发明授权
    Delta-4 desaturase and its use in making polyunsaturated fatty acids 有权
    Delta-4去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08119784B2

    公开(公告)日：2012-02-21

    申请号：US12408860

    申请日：2009-03-23

    Applicant: Quinn Qun Zhu ,  Zhixiong Xue

    Inventor： Quinn Qun Zhu ,  Zhixiong Xue

    IPC: C07H21/04 ,  C12N1/20 ,  C12N5/10

    CPC classification number: C12N9/0083

    Abstract: Described here are Δ4 desaturases that convert all-cis-7,10,13,16,19-docosapentaenoic acid [“DPA”; 22:5 ω-3] to docosahexaenoic acid [“DHA”; 22:6 ω-3], with secondary activity in converting docosatetraenoic acid [“DTA”; 22:4 ω-6] to all-cis-4,7,10,13,16-docosapentaenoic acid [“DPAn-6”; 22:5 ω-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding Δ4 desaturases as well as methods of making long chain polyunsaturated fatty acids [“PUFAs”] using this Δ4 desaturase in oleaginous yeast.

    Abstract translation: 这里描述的是将全顺式-7,10,13,16,19-二十二碳五烯酸[“DPA”; 22：5ω-3]至二十二碳六烯酸[“DHA”; 22：6ω-3]，二次活性转化二十二碳四烯酸[“DTA”; 22：4ω-6]与全顺式-4,7,10,13,16-二十二碳五烯酸[“DPAn-6”; 22：5ω-6]。 此外，这里描述的是分离的核酸片段和包含编码'Dgr。4去饱和酶的这种片段的重组构建体以及使用这种＆Dgr。4去饱和酶在含油酵母中制备长链多不饱和脂肪酸的方法。

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