中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

79 records (took 0.02 seconds)

    SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS
    34.
    发明申请
    SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS 审中-公开
    融合蛋白中的亲和标签的特定标签

    公开(公告)号：US20090081722A1

    公开(公告)日：2009-03-26

    申请号：US12117689

    申请日：2008-05-08

    Applicant: Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    Inventor： Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    IPC: G01N1/30 ,  C07F5/02 ,  C07D311/06

    CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

    Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

    Detection of transmembrane potentials using asymmetric thiobarbituric acid-derived polymethine oxonols
    38.
    发明申请
    Detection of transmembrane potentials using asymmetric thiobarbituric acid-derived polymethine oxonols 审中-公开
    使用不对称硫代巴比妥酸衍生的聚甲基氧杂环丁烷检测跨膜电位

    公开(公告)号：US20050226816A1

    公开(公告)日：2005-10-13

    申请号：US10971311

    申请日：2004-10-21

    Applicant: Zhenjun Diwu ,  Yong Yao ,  Jianjun He ,  Guobing Xiang

    Inventor： Zhenjun Diwu ,  Yong Yao ,  Jianjun He ,  Guobing Xiang

    IPC: A61B5/00 ,  A61B5/05 ,  A61B8/00 ,  A61B10/00 ,  G01N33/50

    CPC classification number: A61B5/0059 ,  G01N33/5005

    Abstract: The present invention relates generally to the detection and measurement of transmembrane potentials using an asymmetric thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonols. The method comprises a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are (a) independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl, and (b) R1, R2 and R3 are not simultaneously methyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

    Abstract translation: 本发明一般涉及使用不对称硫代巴比妥酸衍生的聚甲基氧杂多酚（如下所示）检测和测量跨膜电位。 特别地，本发明涉及使用中等疏水性的不对称硫代巴比妥酸衍生的多聚甲醛氧化物确定生物细胞的质膜跨膜电位的组合物和光学方法。 该方法包括中等疏水性的不对称硫代巴比妥酸衍生的聚甲炔氧杂壬基阴离子，其能够响应于膜的电位变化而从膜的第一面重新分布到膜的第二面。 在一个方面，该方法用于鉴定调节生物膜中膜电位的化合物。 其中R 1，R 2和R 3是（a）独立地选自氢，烷基，卤代烷基和杂烷基，和（b）R 1，R 2和R 3不同时为甲基; n为1〜3的整数， Z是Na，K，铵或其他生物可接受的盐。

    Fluorescent pH indicators for intracellular assays
    39.
    发明申请
    Fluorescent pH indicators for intracellular assays 有权
    用于细胞内测定的荧光pH指标

    公开(公告)号：US20050136503A1

    公开(公告)日：2005-06-23

    申请号：US10958670

    申请日：2004-10-04

    Applicant: Zhenjun Diwu ,  Jesse Twu ,  Guoliang Yi ,  Luke Lavis ,  Yen-Wen Chen ,  Kelly Cassutt

    Inventor： Zhenjun Diwu ,  Jesse Twu ,  Guoliang Yi ,  Luke Lavis ,  Yen-Wen Chen ,  Kelly Cassutt

    IPC: C07D311/82 ,  C07D493/10 ,  G01N1/30 ,  G01N33/84 ,  C12Q1/34 ,  G01N33/48

    CPC classification number: C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

    Abstract: Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2′,7′-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

    Abstract translation: 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和相关的非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示物，照射样品池，以及检测游离指示剂的荧光响应。

Patent Agency Ranking