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    Detection of transmembrane potentials using asymmetric thiobarbituric acid-derived polymethine oxonols
    1.
    发明申请
    Detection of transmembrane potentials using asymmetric thiobarbituric acid-derived polymethine oxonols 审中-公开
    使用不对称硫代巴比妥酸衍生的聚甲基氧杂环丁烷检测跨膜电位

    公开(公告)号：US20050226816A1

    公开(公告)日：2005-10-13

    申请号：US10971311

    申请日：2004-10-21

    申请人： Zhenjun Diwu ,  Yong Yao ,  Jianjun He ,  Guobing Xiang

    发明人： Zhenjun Diwu ,  Yong Yao ,  Jianjun He ,  Guobing Xiang

    IPC分类号： A61B5/00 ,  A61B5/05 ,  A61B8/00 ,  A61B10/00 ,  G01N33/50

    CPC分类号： A61B5/0059 ,  G01N33/5005

    摘要： The present invention relates generally to the detection and measurement of transmembrane potentials using an asymmetric thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonols. The method comprises a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are (a) independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl, and (b) R1, R2 and R3 are not simultaneously methyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

    摘要翻译： 本发明一般涉及使用不对称硫代巴比妥酸衍生的聚甲基氧杂多酚（如下所示）检测和测量跨膜电位。 特别地，本发明涉及使用中等疏水性的不对称硫代巴比妥酸衍生的多聚甲醛氧化物确定生物细胞的质膜跨膜电位的组合物和光学方法。 该方法包括中等疏水性的不对称硫代巴比妥酸衍生的聚甲炔氧杂壬基阴离子，其能够响应于膜的电位变化而从膜的第一面重新分布到膜的第二面。 在一个方面，该方法用于鉴定调节生物膜中膜电位的化合物。 其中R 1，R 2和R 3是（a）独立地选自氢，烷基，卤代烷基和杂烷基，和（b）R 1，R 2和R 3不同时为甲基; n为1〜3的整数， Z是Na，K，铵或其他生物可接受的盐。