公开(公告)号：US11565259B2

公开(公告)日：2023-01-31

申请号：US16683798

申请日：2019-11-14

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US20220379320A1

公开(公告)日：2022-12-01

申请号：US17654938

申请日：2022-03-15

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: B03C5/00 ,  B03C5/02 ,  B03C11/00 ,  B01L3/00

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

公开(公告)号：US11318479B2

公开(公告)日：2022-05-03

申请号：US15105849

申请日：2014-12-18

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: C12Q1/68 ,  B03C5/00 ,  B03C5/02 ,  B03C11/00 ,  B01L3/00

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

公开(公告)号：US10829728B2

公开(公告)日：2020-11-10

申请号：US16010176

申请日：2018-06-15

Applicant: Berkeley Lights, Inc.

Inventor： Volker L. S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US20180099282A1

公开(公告)日：2018-04-12

申请号：US15659855

申请日：2017-07-26

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC: B01L3/00 ,  G01N1/40

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

公开(公告)号：US20170354969A1

公开(公告)日：2017-12-14

申请号：US15487795

申请日：2017-04-14

Applicant: Berkeley Lights,Inc.

Inventor： Troy A. Lionberger ,  Brandon R. Bruhn ,  John A. Tenney ,  Eric D. Hobbs

IPC: B01L3/00 ,  G01N27/447 ,  B03C5/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/0424 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/024 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44791

Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

公开(公告)号：US20160184821A1

公开(公告)日：2016-06-30

申请号：US14961215

申请日：2015-12-07

Applicant: Berkeley Lights, Inc.

Inventor： Eric D. Hobbs ,  Justin K. Valley

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2400/0415 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  H01L27/14681 ,  H01L29/732

Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.

Abstract translation: 微流体装置可以包括底部，其外表面形成用于容纳流体介质的一个或多个外壳。 基极可以包括独立可控晶体管结构的阵列，其中每一个可以包括横向晶体管和垂直晶体管。 晶体管结构可以被光激活，并且横向和垂直晶体管因此可以是光电晶体管。 每个晶体管结构可以被激活以从基底的外表面（并且因此在外壳中的流体介质）的区域到公共电导体产生临时电连接。 临时电连接可以通常在该区域处引起局部电动力，其可以足够强以移动附近的微物体。