公开(公告)号：US10829728B2

公开(公告)日：2020-11-10

申请号：US16010176

申请日：2018-06-15

Applicant: Berkeley Lights, Inc.

Inventor： Volker L. S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US20170354969A1

公开(公告)日：2017-12-14

申请号：US15487795

申请日：2017-04-14

Applicant: Berkeley Lights,Inc.

Inventor： Troy A. Lionberger ,  Brandon R. Bruhn ,  John A. Tenney ,  Eric D. Hobbs

IPC: B01L3/00 ,  G01N27/447 ,  B03C5/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/0424 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/024 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44791

Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

公开(公告)号：US20230182136A1

公开(公告)日：2023-06-15

申请号：US18164124

申请日：2023-02-03

Applicant: BERKELEY LIGHTS, INC.

Inventor： Kristin G. Beaumont ,  Non-Linda Ding ,  Volker L.S. Kurz ,  Troy A. Lionberger ,  Randall D. Lowe, JR. ,  Daniele Malleo ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  B01J19/00 ,  G01N27/447

CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L3/502738 ,  B01J19/0093 ,  B01L3/5023 ,  B01L3/502707 ,  G01N27/44791 ,  B01L2400/0424 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0677 ,  B01L2200/0668 ,  B01L2300/16 ,  B01L2400/08

Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.

公开(公告)号：US20210213444A1

公开(公告)日：2021-07-15

申请号：US16893885

申请日：2020-06-05

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger ,  Brandon R. Bruhn ,  John A. Tenney ,  Eric D. Hobbs

IPC: B01L3/00 ,  G01N27/447 ,  B03C5/00 ,  B03C5/02

Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

公开(公告)号：US20210090252A1

公开(公告)日：2021-03-25

申请号：US17062907

申请日：2020-10-05

Applicant: Berkeley Lights, Inc.

Inventor： Fenglei Du ,  Paul M. Lundquist ,  John A. Tenney ,  Troy A. Lionberger

IPC: G06T7/00 ,  G01N15/14 ,  G01N21/64 ,  G01N27/453 ,  G06T5/50

Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

公开(公告)号：US11789016B2

公开(公告)日：2023-10-17

申请号：US16849811

申请日：2020-04-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger ,  Phillip J. M. Elms ,  Anupam Singhal ,  Randall D. Lowe, Jr. ,  Volker L. S. Kurz ,  Paul M. Lebel

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/68

CPC classification number: G01N33/54366 ,  B01L3/502761 ,  G01N33/6854 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/165 ,  B01L2300/1822 ,  B01L2400/0424 ,  B01L2400/0457 ,  B01L2400/0472 ,  B01L2400/086

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20210270817A1

公开(公告)日：2021-09-02

申请号：US17198833

申请日：2021-03-11

Applicant: Berkeley Lights, Inc.

Inventor： Paul M. Lebel ,  Troy A. Lionberger ,  Kevin T. Chapman

IPC: G01N33/53 ,  B01L3/00

Abstract: Disclosed herein is a method for assaying binding affinity between a first molecule and a second molecule in a micro-fluidic device.

公开(公告)号：US10832404B2

公开(公告)日：2020-11-10

申请号：US16000385

申请日：2018-06-05

Applicant: Berkeley Lights, Inc.

Inventor： Fenglei Du ,  Paul M. Lundquist ,  John A. Tenney ,  Troy A. Lionberger

IPC: G01L3/00 ,  G01N15/00 ,  G01N15/14 ,  G01N21/05 ,  G01N21/17 ,  G01N21/64 ,  G01N27/453 ,  G06T5/50 ,  G06T7/00 ,  G01N15/10 ,  B01L3/00

Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

公开(公告)号：US20160171686A1

公开(公告)日：2016-06-16

申请号：US14963230

申请日：2015-12-08

Applicant: Berkeley Lights, Inc.

Inventor： Fenglei Du ,  Paul M. Lundquist ,  John A. Tenney ,  Troy A. Lionberger

IPC: G06T7/00 ,  G01N27/453 ,  G06T5/50

CPC classification number: G06T7/0012 ,  B01L3/502761 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/6456 ,  G01N27/453 ,  G01N2015/0038 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1445 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2015/1497 ,  G01N2021/056 ,  G01N2021/1765 ,  G01N2201/0635 ,  G01N2201/127 ,  G06T5/50 ,  G06T2207/30024 ,  G06T2207/30101

Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

Abstract translation: 提供了用于在微流体装置中自动检测微物体的方法。 此外，提供了用于在微流体装置中重新定位微物体的方法。 此外，提供了用于分离微流体装置的空间区域中的微物体的方法。

公开(公告)号：US12272048B2

公开(公告)日：2025-04-08

申请号：US17062907

申请日：2020-10-05

Applicant: Berkeley Lights, Inc.

Inventor： Fenglei Du ,  Paul M. Lundquist ,  John A. Tenney ,  Troy A. Lionberger

IPC: G06T7/00 ,  B01L3/00 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/17 ,  G01N21/64 ,  G01N27/453 ,  G06T5/50

Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.