公开(公告)号：US20100285603A1

公开(公告)日：2010-11-11

申请号：US12326972

申请日：2008-12-03

申请人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

发明人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

IPC分类号： G01N33/00

CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

公开(公告)号：US20090246866A1

公开(公告)日：2009-10-01

申请号：US12327854

申请日：2008-12-04

申请人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

发明人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

IPC分类号： C12M3/08

CPC分类号： G01N33/5002

摘要： The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

摘要翻译： 本发明涉及一种用于收集和处理全血样本的取样管。 采样管含有用于全血差异溶血的试剂，其中所述用于差异溶血的试剂包括用于差异溶血的化学药剂和抗凝血剂，并且其中所述采样管是即用型和一次性采样管。 它还涉及在液相色谱法的全血样品的处理中使用所述采样管以及在基于液相色谱的分析中使用在这种采样管中处理的血液样品。

公开(公告)号：US06210901B1

公开(公告)日：2001-04-03

申请号：US08682791

申请日：1996-07-31

申请人： Christoph Seidel ,  Rupert Herrmann ,  Eva Hoess ,  Hans-Georg Batz

发明人： Christoph Seidel ,  Rupert Herrmann ,  Eva Hoess ,  Hans-Georg Batz

IPC分类号： G01N33541

CPC分类号： C07K16/08 ,  A61K39/00 ,  C07K1/04 ,  G01N33/566 ,  G01N33/56988 ,  G01N33/6878

摘要： The invention relates to derivatives of hepatitis C virus amino acid sequences. These derivatives can be used to screen samples, such as blood, to determine if antibodies to hepatitis C virus are present.

摘要翻译： 本发明涉及丙型肝炎病毒氨基酸序列的衍生物。 这些衍生物可用于筛选样品，例如血液，以确定是否存在丙型肝炎病毒的抗体。

公开(公告)号：US5202233A

公开(公告)日：1993-04-13

申请号：US816795

申请日：1992-01-03

申请人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

发明人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

IPC分类号： C12Q1/34 ,  G01N33/58

CPC分类号： C12Q1/34 ,  G01N33/581 ,  C12Q2334/30 ,  G01N2333/924 ,  Y10S435/805

摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue.Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.

摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5和R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

公开(公告)号：US4912258A

公开(公告)日：1990-03-27

申请号：US159422

申请日：1988-02-05

申请人： Hans-Georg Batz ,  Rupert Herrmann

发明人： Hans-Georg Batz ,  Rupert Herrmann

IPC分类号： C07C67/00 ,  C07C209/00 ,  C07C211/52 ,  C07C213/00 ,  C07C215/16 ,  C07C231/00 ,  C07C233/36 ,  C07C233/43 ,  C07C301/00 ,  C07C309/14 ,  C07C309/46 ,  C07C309/48 ,  C07C309/49 ,  C07C311/05 ,  C07D215/18 ,  C07D455/04 ,  C12Q1/28

CPC分类号： C12Q1/28 ,  C07D215/18 ,  C07D455/04 ,  C12Q2326/00

摘要： Fluorinated aniline derivatives of the general formula I are described ##STR1## in which R.sup.1 represents H or (CH.sub.2).sub.n -X, n is a whole number from 1 to 3, X is H, OH, NH.sub.2, CH.sub.3 CONH, CH.sub.3 SO.sub.2 NH, SO.sub.3 H or ArSO.sub.3 H, Ar is optionally substituted arylene radical, R.sup.1 is alternatively also a --(CH.sub.2).sub.3 -- group, which is bonded to the free o-position next to the N-atom, Y is H, --O--, --NH--, --S-- or a C--C single bond, R.sup.2, in the event that Y=H, is the same as or different from R.sup.1 and means one of the radicals shown for R.sup.1, and in the event that Y is a C--C single bond or --O--, --NH-- or --S--, is an alkylene group having 1 to 3 carbon atoms linked with Y, and R.sup.3 =H, alkyl having 1 to 3 carbon atoms, OCH.sub.3, CH.sub.3 CONH, CO.sub.2 H or SO.sub.3 H.The compounds of formula I form as coupling components together with the conventional color couplers a very sensitive chromogenic system which is suitable for the analytical determination of oxidizing substances, for example for the determination of H.sub.2 O.sub.2 and POD in body fluids.

摘要翻译： 描述通式I的氟化苯胺衍生物描述为其中R 1表示H或（CH 2）n X，n为1至3的整数，X为H，OH，NH 2，CH 3 CONH，CH 3 SO 2 NH，SO 3 H 或ArSO 3 H，Ar为任选取代的亚芳基，R 1还可为 - （CH 2）3 - 基团，其与N-原子上的游离邻位键合，Y为H，-O-，-NH- ，-S-或CC单键，在Y = H的情况下，R 2与R 1相同或不同，并且表示R1所示的基团之一，并且在Y为CC单键或 -O-，-NH-或-S-是与Y连接的具有1至3个碳原子的亚烷基，R 3 = H，具有1至3个碳原子的烷基，OCH 3，CH 3 CONH，CO 2 H或SO 3 H。 式I的化合物与常规的色成色剂一起形成耦合组分，这是非常敏感的显色系统，其适用于氧化物质的分析测定，例如用于测定体液中的H 2 O 2和POD。

公开(公告)号：US4737458A

公开(公告)日：1988-04-12

申请号：US894735

申请日：1986-08-08

申请人： Hans-Georg Batz ,  Rupert Herrmann ,  Joachim Siedel

发明人： Hans-Georg Batz ,  Rupert Herrmann ,  Joachim Siedel

IPC分类号： C07D231/46 ,  C12Q1/28 ,  G01N31/00 ,  G01N33/50

CPC分类号： C12Q1/28 ,  C07D231/46 ,  C12Q2326/96

摘要： The present invention provides aminopyrazolinones of the general formula: ##STR1## wherein R.sup.1, R.sup.2 and R.sup.3, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 carbon atoms and together containing at most 8 carbon atoms.The present invention also provides a process for the preparation of these new aminopyrazolinones and reagents containing them. In addition, the present invention is concerned with the use of these new aminopyrazolinones as a color coupler for the detection of hydrogen peroxide.

摘要翻译： 本发明提供了以下通式的氨基吡唑啉酮：其中R 1，R 2和R 3可以相同或不同，是含有至多6个碳原子并且一起含有至多8个碳原子的直链或支链烷基 。 本发明还提供了制备这些新的氨基吡唑啉酮和含有它们的试剂的方法。 此外，本发明涉及这些新的氨基吡唑啉酮作为彩色成色剂用于检测过氧化氢的用途。

公开(公告)号：US07815803B2

公开(公告)日：2010-10-19

申请号：US12133545

申请日：2008-06-05

申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

IPC分类号： B01D15/08 ,  B01D59/44 ,  H01J49/00

CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。

公开(公告)号：US20090253210A1

公开(公告)日：2009-10-08

申请号：US12328890

申请日：2008-12-05

申请人： Uwe Kobold ,  Thomas Duelffer ,  Rupert Herrmann ,  Herbert von der Eltz

发明人： Uwe Kobold ,  Thomas Duelffer ,  Rupert Herrmann ,  Herbert von der Eltz

IPC分类号： G01N33/48 ,  H01J49/00

CPC分类号： G01N30/06 ,  G01N30/603 ,  G01N30/7233 ,  G01N33/5002 ,  G01N2030/027 ,  Y10T436/24 ,  Y10T436/25

摘要： The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

摘要翻译： 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑包含红细胞并且怀疑或已知包含真核细胞的液体样品中的分析物的方法，所述方法包括以下步骤：在适合于裂解细胞膜的条件下用膜增溶剂处理所述液体样品 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能包含红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。

公开(公告)号：US20090090855A1

公开(公告)日：2009-04-09

申请号：US12133545

申请日：2008-06-05

申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

IPC分类号： H01J49/00 ,  B01D15/26

CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。

公开(公告)号：US07335401B2

公开(公告)日：2008-02-26

申请号：US10774325

申请日：2004-07-12

申请人： Andreas Finke ,  Ursula Klause ,  Federic Donie ,  Rupert Herrmann ,  Herbert Von Der Eltz ,  Peter Sluka ,  Wolfgang Jona

发明人： Andreas Finke ,  Ursula Klause ,  Federic Donie ,  Rupert Herrmann ,  Herbert Von Der Eltz ,  Peter Sluka ,  Wolfgang Jona

IPC分类号： B05D7/00 ,  G01N33/553 ,  G01N33/546

CPC分类号： G01N33/54326 ,  A61K9/1676 ,  G01N33/54353 ,  G01N33/545

摘要： The present invention concerns a method for producing microparticles loaded with proteins which is characterized in that the microparticles are loaded in suspension under strongly alkaline conditions. The invention also concerns microparticles which can be produced using this method and their use in a binding test e.g. in an immunoassay.

摘要翻译： 本发明涉及一种负载蛋白质的微粒的制造方法，其特征在于，在强碱性条件下将微粒负载在悬浮液中。 本发明还涉及可以使用该方法生产的微粒及其在结合试验中的用途，例如， 在免疫测定中。