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    Aminopyrazolinones, reagent containing them and the use thereof in the enzymatic determinaton of hydrogen peroxide
    1.
    发明授权
    Aminopyrazolinones, reagent containing them and the use thereof in the enzymatic determinaton of hydrogen peroxide 失效
    氨基吡唑啉酮，含有它们的试剂及其在过氧化氢酶的测定中的应用

    公开(公告)号：US4737458A

    公开(公告)日：1988-04-12

    申请号：US894735

    申请日：1986-08-08

    申请人： Hans-Georg Batz ,  Rupert Herrmann ,  Joachim Siedel

    发明人： Hans-Georg Batz ,  Rupert Herrmann ,  Joachim Siedel

    IPC分类号： C07D231/46 ,  C12Q1/28 ,  G01N31/00 ,  G01N33/50

    CPC分类号： C12Q1/28 ,  C07D231/46 ,  C12Q2326/96

    摘要： The present invention provides aminopyrazolinones of the general formula: ##STR1## wherein R.sup.1, R.sup.2 and R.sup.3, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 carbon atoms and together containing at most 8 carbon atoms.The present invention also provides a process for the preparation of these new aminopyrazolinones and reagents containing them. In addition, the present invention is concerned with the use of these new aminopyrazolinones as a color coupler for the detection of hydrogen peroxide.

    摘要翻译： 本发明提供了以下通式的氨基吡唑啉酮：其中R 1，R 2和R 3可以相同或不同，是含有至多6个碳原子并且一起含有至多8个碳原子的直链或支链烷基 。 本发明还提供了制备这些新的氨基吡唑啉酮和含有它们的试剂的方法。 此外，本发明涉及这些新的氨基吡唑啉酮作为彩色成色剂用于检测过氧化氢的用途。

    Creatinine antibody
    2.
    发明授权
    Creatinine antibody 失效
    肌酸酐抗体

    公开(公告)号：US4578361A

    公开(公告)日：1986-03-25

    申请号：US656957

    申请日：1984-10-02

    申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  A61K39/00

    CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

    摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

    摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

    Process and reagent for the determination of creatinine
    3.
    发明授权
    Process and reagent for the determination of creatinine 失效
    用于测定肌酐的方法和试剂

    公开(公告)号：US4543325A

    公开(公告)日：1985-09-24

    申请号：US449925

    申请日：1982-12-15

    申请人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    发明人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    IPC分类号： G01N33/53 ,  G01N33/70 ,  C12N9/96 ,  C12Q1/34

    CPC分类号： G01N33/70 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/822

    摘要： The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the general formula: ##STR2## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance, a conjugate of a hydantoin of general formula (I) with a second hapten carrier substance which does not cross-react substantially with the first hapten carrier substance and a buffer substance.

    摘要翻译： 本发明提供了一种免疫学测定肌酐的方法，其中将肌酐转化为1-甲基乙内酰脲，所形成的1-甲基乙内酰脲在水性介质中与针对通式 ：其中可以相同或不同的R 1，R 2，R 3和R 4为氢原子，含有至多3个碳原子的烷基或苯基，其中第一半抗原载体物质适用于抗体 与通式（I）的第二乙内酰脲衍生物与第二半抗原载体物质的缀合物反应，抗体和缀合物之一是固相或溶解形式存在，另一种成分以溶解形式存在 并且测量了与第二半抗原载体物质的抗体和乙内酰脲缀合物之间的结合反应的抑制。 本发明还提供了一种用于免疫测定肌酐的试剂，其中含有肌酐亚氨基水解酶，针对通式如下的乙内酰脲的缀合物的抗体：其中R1，R2，R3和R4可以相同 或不同的是氢原子，含有至多3个碳原子的烷基或苯基，具有第一半抗原载体物质，通式（I）的乙内酰脲与不会基本上交叉反应的第二半抗原载体物质的缀合物 具有第一半抗原载体物质和缓冲物质。

    Process and reagent for the determination of substrates or enzyme activities
    4.
    发明授权
    Process and reagent for the determination of substrates or enzyme activities 失效
    用于测定底物或酶活性的方法和试剂

    公开(公告)号：US5196314A

    公开(公告)日：1993-03-23

    申请号：US661271

    申请日：1991-02-26

    申请人： Michael-Harold Town ,  Joachim Siedel ,  Joachim Ziegenhorn

    发明人： Michael-Harold Town ,  Joachim Siedel ,  Joachim Ziegenhorn

    IPC分类号： C07D257/04 ,  C12Q1/26 ,  C12Q1/28

    CPC分类号： C07D257/04 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/92 ,  Y10S435/805

    摘要： A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added tetrazolium salts to remove disturbing substances. The tetrazolium salts have the formula ##STR1## in which R.sup.1 is a hydrogen atom, a carboxyl group or an alkyl, phenyl, nitrophenyl, dinitrophenyl, carboxyl-substituted phenyl or trialkylammoniumphenyl radical, R.sup.2 is a phenyl, nitrophenyl, biphenylyl or naphthyl radical, R.sup.3 is a phenyl, carboxyl-substituted phenyl, carboxyl-substituted hydroxyphenyl or dimethylthiazolyl radical, and A.sup..crclbar. is a monovalent anion. The formazanes formed by reaction with reducing substances do not absorb light at all, or absorb light only to a negligible extent, at the measurement wavelength of the redox reaction.New compounds included within the structural formula are those in which R.sup.1 is carboxy, phenyl, 2-carboxyphenyl, 4-carboxyphenyl, 2,4-dinitrophenyl or 4-trimethylammoniumphenyl, R.sup.2 is phenyl, and R.sup.3 is phenyl or 2-carboxyphenyl; with the proviso that, when R.sup.3 is unsubstituted phenyl or 2-carboxyphenyl, R.sup.1 may not be unsubstituted phenyl.

    摘要翻译： 通过使用氧化还原反应作为测量反应来测定底物或酶活性的方法在一种或多种另外加入的四唑鎓盐的存在下进行以除去扰乱物质。 所述四唑鎓盐具有其中R 1为氢原子，羧基或烷基，苯基，硝基苯基，二硝基苯基，羧基取代的苯基或三烷基铵苯基的式（I），R 2为苯基，硝基苯基，联苯基或 萘基，R3是苯基，羧基取代的苯基，羧基取代的羟基苯基或二甲基噻唑基，A（ - ）是一价阴离子。 通过与还原物质反应而形成的甲烷在氧化还原反应的测定波长下根本不吸收光，或仅吸收光可忽略的程度。 包括在结构式中的新化合物是其中R1是羧基，苯基，2-羧基苯基，4-羧基苯基，2,4-二硝基苯基或4-三甲基铵苯基，R2是苯基，R3是苯基或2-羧基苯基的化合物; 条件是当R3是未取代的苯基或2-羧基苯基时，R 1可以不是未被取代的苯基。

    Method for determining iron in a body fluid sample
    5.
    发明授权
    Method for determining iron in a body fluid sample 失效
    用于测定体液样品中铁的方法

    公开(公告)号：US4961970A

    公开(公告)日：1990-10-09

    申请号：US238620

    申请日：1988-08-30

    申请人： Joachim Siedel ,  Lieselotte Schellong ,  Johnny Staepels ,  Uwe Herrmann ,  Michael-Harold Town

    发明人： Joachim Siedel ,  Lieselotte Schellong ,  Johnny Staepels ,  Uwe Herrmann ,  Michael-Harold Town

    IPC分类号： G01N33/84

    CPC分类号： G01N33/84 ,  Y10S436/91

    摘要： The present invention provides a process for the determination of iron in body fluids by liberation of the bound iron, reduction to Fe.sup.2+, addition of a color system appropriate for the detection of iron and photometric measurement in a tenside-containing sample solution, wherein a fatty acid polyethylene glycol ester, an alkanol polyglycol ether and at least 1 mole/liter quanidine hydrochloride are added to the sample solution. The present invention also provides a reagent for the determination of iron in serum, containing a reducing agent, a color material system appropriate for the detection of iron and guanidine hydrochloride and at least one tenside, wherein it contains a fatty acid polyethylene glycol ester and a alkanol polyglycol ether.

    摘要翻译： 本发明提供了一种通过释放结合的铁，还原成Fe2 +，添加适合于检测铁的颜色系统和在含有表面活性剂的样品溶液中进行光度测量来测定体液中铁的方法，其中脂肪 酸性聚乙二醇酯，链烷醇聚乙二醇醚和至少1摩尔/升盐酸氢化物加入到样品溶液中。 本发明还提供了一种用于测定血清中含铁的试剂，其含有还原剂，适用于检测铁和胍盐酸盐和至少一种表面活性剂的着色材料体系，其中它含有脂肪酸聚乙二醇酯和 链烷醇聚乙二醇醚。

    Process for DNA decontamination
    8.
    发明申请
    Process for DNA decontamination 审中-公开
    DNA去污工艺

    公开(公告)号：US20070148035A1

    公开(公告)日：2007-06-28

    申请号：US11564441

    申请日：2006-11-29

    申请人： Armin Tgetgel ,  Joachim Siedel ,  Alois Rainer ,  Wolf-Dieter Engel

    发明人： Armin Tgetgel ,  Joachim Siedel ,  Alois Rainer ,  Wolf-Dieter Engel

    IPC分类号： C12M1/34 ,  A61L2/20

    CPC分类号： A61L2/206

    摘要： A method and a disposable to avoid false nucleic acid amplification results based on the alklylation of DNA present at surfaces of disposables. More particularly, the present invention is directed to an improved method and a disposable, whereby the alkylation of DNA is performed using ethylene oxide.

    摘要翻译： 基于存在于一次性物品表面的DNA的烷基化反应的方法和一次性以避免假核酸扩增结果。 更具体地说，本发明涉及一种改进的方法和一次性物质，由此使用环氧乙烷进行DNA的烷基化。

    Reagent for specific determination of fructosamine
    9.
    发明授权
    Reagent for specific determination of fructosamine 失效
    具体测定果糖胺的试剂

    公开(公告)号：US5288606A

    公开(公告)日：1994-02-22

    申请号：US911282

    申请日：1992-07-09

    申请人： Joachim Siedel ,  Joachim Ziegenhorn ,  Lieselotte Schellong ,  Bernd Vogt

    发明人： Joachim Siedel ,  Joachim Ziegenhorn ,  Lieselotte Schellong ,  Bernd Vogt

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/30 ,  C12Q1/44 ,  C12Q1/54 ,  G01N33/66 ,  C12Q1/00 ,  A61K35/78 ,  A61K37/02 ,  C12P19/38

    CPC分类号： C12Q1/26 ,  G01N33/66 ,  Y10S436/825 ,  Y10S436/904 ,  Y10T436/144444 ,  Y10T436/25125

    摘要： The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.

    摘要翻译： 本发明提供一种用于通过与适当的着色试剂反应并测量由此引起的颜色变化来特异性测定血液或血液中衍生血液的血清果糖胺含量的方法，其中在显色反应之前，非特异性还原 在大约中性pH值下去除起泡和/或浊度的样品组分，随后将pH调节至10至12的值，并向其中加入着色剂。 本发明还提供了用于特异性测定血液或血液中衍生血液中的果糖胺含量的试剂混合物，其中它包括用于除去非特异性还原作用和/或引起浊度的样品组分的试剂， 具有pH值在10.5〜12.5范围内的缓冲液和用于检测果糖胺的显色试剂的缓冲试剂。

    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof
    10.
    发明授权
    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
    核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途

    公开(公告)号：US4816393A

    公开(公告)日：1989-03-28

    申请号：US704712

    申请日：1985-02-25

    申请人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    发明人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    IPC分类号： C12N9/14 ,  C12N9/86 ,  C12Q1/34 ,  C12R1/01 ,  C12R1/06 ,  C12R1/13 ,  C12R1/265 ,  G01N33/70 ,  C12Q1/26 ,  C12Q1/32

    CPC分类号： C12N9/86 ,  C12Q1/34 ,  G01N33/70 ,  G01N2333/986 ,  Y10S435/81

    摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.

    摘要翻译： 本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。 本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。 此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解， 测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。