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    DETECTION OF IMMOBILIZED NUCLEIC ACID
    32.
    发明申请
    DETECTION OF IMMOBILIZED NUCLEIC ACID 有权

    公开(公告)号：US20100216665A1

    公开(公告)日：2010-08-26

    申请号：US12771897

    申请日：2010-04-30

    申请人： Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    发明人： Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    IPC分类号： C40B30/04 ,  G01N33/00

    CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    Methine-substituted cyanine dye compounds
    33.
    发明授权
    Methine-substituted cyanine dye compounds 有权
    甲基取代的花青染料化合物

    公开(公告)号：US07776529B2

    公开(公告)日：2010-08-17

    申请号：US11005861

    申请日：2004-12-06

    申请人： Jason Alfred Dallwig ,  David Carl Hagen ,  Ching-Ying Cheung ,  Gerald Alan Thomas ,  Stephen Yue

    发明人： Jason Alfred Dallwig ,  David Carl Hagen ,  Ching-Ying Cheung ,  Gerald Alan Thomas ,  Stephen Yue

    IPC分类号： A61K31/4709 ,  C07D215/12 ,  C12Q1/68

    CPC分类号： C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

    摘要： Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

    摘要翻译： 具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

    APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS
    34.
    发明申请
    APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS 有权
    用于自偏置具有用于低电压应用的多模输出配置的差分信号电路的装置和方法

    公开(公告)号：US20070268043A1

    公开(公告)日：2007-11-22

    申请号：US11830897

    申请日：2007-07-31

    申请人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard Fung

    发明人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard Fung

    IPC分类号： H03K19/20 ,  H03K19/094

    CPC分类号： H03K17/302 ,  H03K17/04106

    摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.

    摘要翻译： 本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。 两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。 电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。 开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。 此外，体积偏置电路被包括并且可操作地耦合到开关。 体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

    APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS
    35.
    发明申请
    APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS 有权
    用于自偏置具有用于低电压应用的多模输出配置的差分信号电路的装置和方法

    公开(公告)号：US20060284649A1

    公开(公告)日：2006-12-21

    申请号：US11160243

    申请日：2005-06-15

    申请人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard Fung

    发明人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard Fung

    IPC分类号： H03K19/094

    CPC分类号： H03K17/302 ,  H03K17/04106

    摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.

    摘要翻译： 本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。 两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。 电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。 开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。 此外，体积偏置电路被包括并且可操作地耦合到开关。 体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

    Seamless steel tube manufacture
    36.
    发明授权
    Seamless steel tube manufacture 失效
    无缝钢管制造

    公开(公告)号：US5186769A

    公开(公告)日：1993-02-16

    申请号：US751078

    申请日：1991-07-28

    申请人： Patrick J. Hunt ,  John J. Jonas ,  Stephen Yue ,  George E. Ruddle ,  Lakshman N. Pussegoda

    发明人： Patrick J. Hunt ,  John J. Jonas ,  Stephen Yue ,  George E. Ruddle ,  Lakshman N. Pussegoda

    IPC分类号： C21D6/02 ,  C21D8/10

    CPC分类号： C21D6/02 ,  C21D8/10

    摘要： A method for producing seamless steel tubes suitable for use as grades of casing and line pipe having yield strengths in excess of 70,000 psi, without being heat treated. Such steels are made of an alloy comprising, by weight, about 0.10% to 0.18% carbon, about 0.10% to 2.0% manganese, about 0.10% to 0.16% vanadium, about 0.008% to 0.012% titanium and about 150 parts per million to 220 parts per million nitrogen, the balance comprising iron and incidental impurities. The subject method comprises the steps of alloying a steel of the aforesaid chemical composition, forming the steel into a billet of steel, reheating the billet in a reheating furnace, passing the billet through a piercing mill to form a steel shell, elongating the steel shell within a mandrel mill, and reducing the diameter of the elongated shell in a stretch reducing mill. Strains are applied to the shell in the stretch reducing mill below the T.sub.nr of the steel and above the A.sub.r3 to provoke dynamic recrystallization. The subject method may also include the steps of cooling the steel shell after it exits the mandrel mill to a temperature below its A.sub.r1 temperature, prior to reheating, and/or accelerated cooling (optional, special cooling may not be necessary for thinner walls) after exiting the stretch reducing mill at a rate between 3.degree. C. to 5.degree. C. per second. The nitrogen and vanadium are preferably introduced to the steel during alloying in the form of a VN alloying agent.

    摘要翻译： 适用于不经热处理的屈服强度超过70,000psi的套管和管线等级的无缝钢管的生产方法。 这种钢由合金组成，包括约0.10％至0.18％的碳，约0.10％至2.0％的锰，约0.10％至0.16％的钒，约0.008％至0.012％的钛和约150ppm的钛 220百万分之氮，余量由铁和附带杂质组成。 本发明的方法包括以下步骤：将上述化学组成的钢合金化，将钢形成钢坯，在再加热炉中重新加热坯料，使坯料通过穿孔机形成钢壳，使钢壳伸长 在芯棒式无缝管轧机中，并且在减速磨机中减小细长壳体的直径。 将菌株施加到钢的Tnr下方的拉伸还原轧机的壳体上，并且在Ar 3上方引起动态再结晶。 本发明的方法还可以包括以下步骤：在再次加热和/或加速冷却之后将钢壳冷却到低于其Ar1温度的温度下冷却（可选地，对于较薄壁而言不需要特殊的冷却） 以3℃至5℃/秒的速率离开拉伸还原研磨机。 氮和钒优选在VN合金化剂形式的合金化过程中引入钢中。