公开(公告)号：US10464061B2

公开(公告)日：2019-11-05

申请号：US15096933

申请日：2016-04-12

Applicant: International Business Machines Corporation

Inventor： Jingwei Bai ,  Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B81C1/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  C12Q1/6874 ,  B82Y40/00

Abstract: A technique includes forming a gradient channel with width and depth gradients. A mask is disposed on top of a substrate. The mask is patterned with at least one elongated channel pattern having different elongated channel pattern widths. A channel is etched in the substrate in a single etching step, the channel having a width gradient and a corresponding depth gradient both simultaneously etched in the single etching step according to the different elongated channel pattern widths in the mask.

公开(公告)号：US09983190B2

公开(公告)日：2018-05-29

申请号：US15085394

申请日：2016-03-30

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: G01N33/487 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

公开(公告)号：US09290806B2

公开(公告)日：2016-03-22

申请号：US14608675

申请日：2015-01-29

Applicant: International Business Machines Corporation

Inventor： Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: C12Q1/68 ,  B82Y15/00 ,  B82Y40/00 ,  B81C1/00 ,  B82Y5/00

CPC classification number: C12Q1/6874 ,  B81C1/00087 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/723 ,  Y10S977/74 ,  Y10S977/742 ,  Y10S977/781 ,  Y10S977/904 ,  Y10S977/92 ,  Y10S977/953 ,  Y10S977/958 ,  C12Q2565/631

Abstract: A technique is provided for a structure. A substrate has a nanopillar vertically positioned on the substrate. A bottom layer is formed beneath the substrate. A top layer is formed on top of the substrate and on top of the nanopillar, and a cover layer covers the top layer and the nanopillar. A window is formed through the bottom layer and formed through the substrate, and the window ends at the top layer. A nanopore is formed through the top layer by removing the cover layer and the nanopillar.

Abstract translation: 为结构提供了一种技术。 衬底具有垂直定位在衬底上的纳米柱。 在基底下方形成底层。 在衬底的顶部和纳米柱的顶部上形成顶层，并且覆盖层覆盖顶层和纳米柱。 通过底层形成窗口并形成穿过基底，并且窗口在顶层结束。 通过去除覆盖层和纳米柱，通过顶层形成纳米孔。

公开(公告)号：US09194860B2

公开(公告)日：2015-11-24

申请号：US13675389

申请日：2012-11-13

Applicant: International Business Machines Corporation

Inventor： Hongbo Peng ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: G01N33/487 ,  C12Q1/68 ,  B82Y30/00

CPC classification number: G01N33/48721 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device.

公开(公告)号：US20150285764A1

公开(公告)日：2015-10-08

申请号：US14585236

申请日：2014-12-30

Applicant: International Business Machines Corporation

Inventor： Gustavo A. Stolovitzky ,  George F. Walker ,  Chao Wang ,  Deqiang Wang

IPC: G01N27/447 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12N15/101 ,  G01N27/44747 ,  G01N27/44791 ,  C12Q2527/125 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/631

Abstract: A mechanism is provided for reducing entropy of a polyelectrolyte before the polyelectrolyte moves through a nanopore. A free-standing membrane has the nanopore formed through the membrane. An agarose gel is formed onto either and/or both sides of the nanopore in the membrane. The agarose gel is a porous material. The polyelectrolyte is uncoiled by driving the polyelectrolyte through the porous material of the agarose gel via an electric field. Driving the polyelectrolyte, having been uncoiled and linearized by the agarose gel, into the nanopore is for sequencing.

Abstract translation: 提供了一种用于在聚电解质通过纳米孔移动之前降低聚电解质的熵的机制。 独立的膜具有通过膜形成的纳米孔。 在膜中的纳米孔的两侧和/或两侧上形成琼脂糖凝胶。 琼脂糖凝胶是多孔材料。 通过电场通过琼脂糖凝胶的多孔材料驱动聚电解质来解开聚电解质。 将已经通过琼脂糖凝胶开卷并线性化的聚电解质驱入纳米孔用于测序。

公开(公告)号：US08969118B2

公开(公告)日：2015-03-03

申请号：US13971442

申请日：2013-08-20

Applicant: International Business Machines Corporation

Inventor： Ali Afzali-Ardakani ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: H01L21/00 ,  G01N27/414 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/02 ,  G01N33/487

CPC classification number: G01N27/4146 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/02 ,  G01N33/48721 ,  Y10S977/958

Abstract: A mechanism is provided for base recognition of an integrated transistor and nanochannel. A target molecule is forced down to a carbon nanotube a single base at a time in the nanochannel by applying a gate voltage to a top electrode, and/or a narrow thickness of the nanochannel. The nanochannel exposes an exposed portion of the carbon nanotube at a bottom wall, and the top electrode is positioned over the exposed portion. The exposed portion of the carbon nanotube is smaller than the distance between bases to only accommodate the single base at a time. The target molecule is stretched by the narrow thickness and by applying a traverse voltage across a length direction of the nanochannel. The target molecule is frictionally restricted by the narrow thickness of the nanochannel to stretch is restrictedly translocates in the length direction. Current is measured to determine an identity of the single base.

公开(公告)号：US20150038691A1

公开(公告)日：2015-02-05

申请号：US14012212

申请日：2013-08-28

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Binquan Luan ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: C12N15/11

CPC classification number: C12P19/34 ,  C12N15/11 ,  C12Q1/6806 ,  Y10T436/143333 ,  C12Q2565/131 ,  C12Q2565/631

Abstract: A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide.

公开(公告)号：US20150037787A1

公开(公告)日：2015-02-05

申请号：US13956033

申请日：2013-07-31

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Binquan Luan ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: C12Q1/68

CPC classification number: C12P19/34 ,  C12N15/11 ,  C12Q1/6806 ,  Y10T436/143333 ,  C12Q2565/131 ,  C12Q2565/631

Abstract: A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide.

公开(公告)号：US20150021187A1

公开(公告)日：2015-01-22

申请号：US13944552

申请日：2013-07-17

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B01L3/00 ,  G01N27/447

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

Abstract translation: 用于通过生物聚合物分子的装置包括形成在表面起伏结构，形成纳米通道的侧壁的图案化层和形成在图案化层上以封装纳米通道的密封层之间的纳米通道。 表面浮雕结构包括三维圆形表面，其减小了在纳米通道的一部分处的纳米通道的通道尺寸，并且将纳米通道的尺寸逐渐增加到构造成接收生物聚合物的开口位置。

公开(公告)号：US08890121B1

公开(公告)日：2014-11-18

申请号：US13887804

申请日：2013-05-06

Applicant: International Business Machines Corporation

Inventor： Shu-Jen Han ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: H01L29/06

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/631

Abstract: A technique is provided for base recognition in an integrated device is provided. A target molecule is driven into a nanopore of the integrated device. The integrated device includes a nanowire separated into a left nanowire part and a right nanowire part to form a nanogap in between, a source pad connected to the right nanowire part, a drain pad connected to the left nanowire part, and the nanopore. The source pad, the drain pad, the right nanowire part, the left nanowire part, and the nanogap together form a transistor. The nanogap is part of the nanopore. A transistor current is measured while a single base of the target molecule is in the nanogap of the nanopore, and the single base affects the transistor current. An identity of the single base is determined according to a change in the transistor current.

Abstract translation: 提供了一种用于集成设备中的基础识别的技术。 靶分子被驱动到集成器件的纳米孔中。 集成器件包括分离成左纳米线部分的纳米线和右纳米线部分，以在其间形成纳米间隙，连接到右纳米线部分的源极焊盘，连接到左纳米线部分的漏极焊盘和纳米孔。 源极焊盘，漏极焊盘，右侧的纳米线部分，左侧的纳米线部分和纳米光栅一起形成晶体管。 纳米孔是纳米孔的一部分。 测量晶体管电流，同时目标分子的单个碱基位于纳米孔的纳米隙中，单个碱基影响晶体管电流。 根据晶体管电流的变化确定单个基极的身份。