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46 条记录 (耗时 0.075 秒)

    Plasmids without a selection marker gene
    35.
    发明授权
    Plasmids without a selection marker gene 失效
    没有选择标记基因的质粒

    公开(公告)号：US06265218B1

    公开(公告)日：2001-07-24

    申请号：US08793618

    申请日：1997-06-10

    申请人： Stefan Seeber ,  Rudiger Ruger

    发明人： Stefan Seeber ,  Rudiger Ruger

    IPC分类号： C12N1500

    CPC分类号： C12N15/65 ,  A61K48/00 ,  C07K14/4712 ,  C12N15/10

    摘要： The use of a circular vector DNA to produce a pharmaceutical agent for the treatment of mammals or humans by gene therapy wherein the vector DNA contains a selection marker gene and a DNA sequence that is heterologous for the vector which causes a modulation, correction or activation of the expression of an endogenous gene or the expression of a gene introduced into the cells of the mammal or the human by the vector DNA which is characterized in that the vector nucleic acid a) is amplified under selection pressure and cleaved in such a way that the selection marker gene and the heterologous DNA are present on separate DNA fragments, b) the DNA fragment which contains the heterologous DNA or both fragments are recircularized to form vectors, c) the DNA fragments are separated before or after the recircularization d) the recircularized DNA fragment which contains the heterologous DNA is isolated and e) the recircularized DNA fragment obtained in this manner is used to produce the pharmaceutical agent.

    摘要翻译： 使用圆形载体DNA通过基因治疗产生用于治疗哺乳动物或人类的药剂，其中载体DNA含有选择标记基因和对引发调节，校正或激活的载体异源的DNA序列 内源基因的表达或通过载体DNA引入哺乳动物或人的细胞中的基因的表达，其特征在于载体核酸a）在选择压力下扩增并以选择的方式被切割 标记基因和异源DNA存在于单独的DNA片段上，b）将含有异源DNA或两个片段的DNA片段再循环形成载体，c）在再循环之前或之后分离DNA片段）再循环DNA片段， 含有异源DNA被分离并且以这种方式获得的再循环DNA片段用于生产药物 代理人

    Recombinant restriction enzyme Sau3AI
    36.
    发明授权
    Recombinant restriction enzyme Sau3AI 失效
    重组限制酶SAU3AI

    公开(公告)号：US5175101A

    公开(公告)日：1992-12-29

    申请号：US712833

    申请日：1991-06-10

    申请人： Friedrich Gotz ,  Stefan Seeber

    发明人： Friedrich Gotz ,  Stefan Seeber

    IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12N15/70 ,  C12N15/74 ,  C12R1/19 ,  C12R1/44 ,  C12R1/445

    CPC分类号： C12N9/1007 ,  C12N15/70 ,  C12N15/74 ,  C12N9/22

    摘要： The invention concerns a DNA which contains(1) at least one of the two regions of the nucleotide sequence SEQ ID NO:1 coding for a protein with a Sau3AI methylase activity or for a protein with a Sau3AI endonuclease activity.(2) a sequence corresponding to the DNA from (1) within the scope of the degeneration of the genetic code or(3) a sequence which hybridizes under stringent hybridization conditions with a DNA from (1) or (2).In addition a vector is disclosed which contains a DNA according to the present invention, in particular under the control of a regulatable closed promoter, as well as microorganisms which are transformed with one or several vectors according to the present invention and the isolation of recombinant Sau3AI endonuclease from the transformed microorganisms.

    摘要翻译： 本发明涉及包含（1）编码具有Sau3AI甲基化酶活性的蛋白质的核苷酸序列SEQ ID NO：1的两个区域中的至少一个或具有Sau3AI核酸内切酶活性的蛋白质的DNA。 （2）对应于遗传密码子退化范围内的（1）的DNA的序列，或（3）在严格杂交条件下与（1）或（2）的DNA杂交的序列。 此外，公开了一种载体，其包含根据本发明的DNA，特别是在可调节封闭启动子的控制下，以及用根据本发明的一种或多种载体转化的微生物以及分离重组Sau3AI 来自转化的微生物的内切核酸酶。

    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor
    39.
    发明授权
    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor 失效
    重组表达肝细胞生长因子N-末端片段的方法

    公开(公告)号：US07754448B2

    公开(公告)日：2010-07-13

    申请号：US10591045

    申请日：2005-03-02

    申请人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    发明人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/4753 ,  C12N15/70

    摘要： A method for the production of the α-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.

    摘要翻译： 通过在微生物宿主细胞中表达编码所述NK多肽的核酸，通过将包含所述NK多肽的包含体分离成变性来生产肝细胞生长因子或其N-末端片段（NK多肽）的α-链的方法 形式，包涵体的溶解和变性NK多肽的生成，其特征在于在所述核酸中，选自33,35和36位的密码子中的至少一个氨基酸的密码子是CGT， 导致表达产率提高。