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公开(公告)号：US10920235B2
公开(公告)日：2021-02-16
申请号：US16723853
申请日：2019-12-20
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
IPC: B26D3/18 , B26D7/06 , B26D7/18 , C12N15/82 , C12N5/00 , C12N9/10 , C12R1/41 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US10091957B2
公开(公告)日：2018-10-09
申请号：US14339934
申请日：2014-07-24
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US20180148730A1
公开(公告)日：2018-05-31
申请号：US15826540
申请日：2017-11-29
Applicant: Monsanto Technology, LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US09885053B2
公开(公告)日：2018-02-06
申请号：US14578112
申请日：2014-12-19
Applicant: Monsanto Technology LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20150121574A1
公开(公告)日：2015-04-30
申请号：US14578112
申请日：2014-12-19
Applicant: Monsanto Technology LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
IPC: C12N15/82
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
Abstract translation: 本发明涉及从棉花种子切除包含分生组织的外植体材料。 公开了转化植物的组织制备，储存，转化和选择或鉴定的方法，以及通过这些方法产生的可转化的分生组织和植物，以及组织制备。
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Patent Agency Ranking
公开(公告)号：US20150082497A1
公开(公告)日：2015-03-19
申请号：US14339934
申请日：2014-07-24
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
IPC: A01H4/00
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US08937216B2
公开(公告)日：2015-01-20
申请号：US13727575
申请日：2012-12-26
Applicant: Monsanto Technology LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
Abstract translation: 本发明涉及从棉花种子切除包含分生组织的外植体材料。 公开了转化植物的组织制备，储存，转化和选择或鉴定的方法，以及通过这些方法产生的可转化的分生组织和植物，以及组织制备。
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公开(公告)号：US12195736B2
公开(公告)日：2025-01-14
申请号：US18481464
申请日：2023-10-05
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , A01H4/00 , C12N5/00 , C12N5/04 , C12N9/10 , G01N33/00 , A01H6/20 , A01H6/46 , A01H6/54 , A01H6/60 , C12N1/20 , C12R1/41
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US20240093222A1
公开(公告)日：2024-03-21
申请号：US18480381
申请日：2023-10-03
Applicant: Monsanto Technology LLC
Inventor： Yurong Chen , Brian J. Martinell , Anatoly Rivlin , Ashok Shrawat , Yuechun Wan , Edward J. Williams , Xudong Ye
IPC: C12N15/82
CPC classification number: C12N15/8275 , C12N15/8274
Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.
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公开(公告)号：US11807858B2
公开(公告)日：2023-11-07
申请号：US17118539
申请日：2020-12-10
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , C12N5/00 , A01H4/00 , C12N5/04 , G01N33/00 , C12N9/10 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60 , C12N1/20 , C12R1/41
CPC classification number: C12N15/8209 , A01H4/003 , A01H4/008 , C12N5/0025 , C12N5/04 , C12N9/1029 , C12N15/8201 , C12N15/8202 , C12N15/8205 , C12N15/8221 , C12N15/8265 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , G01N33/0098 , A01H6/202 , A01H6/4684 , A01H6/542 , A01H6/604 , C12N1/205 , C12N15/8261 , C12R2001/41 , C12Y203/01081
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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