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31.发明申请公开(公告)号：US20060034829A1
公开(公告)日：2006-02-16
申请号：US11187079
申请日：2005-07-21
申请人： Tillman Gerngross , Huijuan Li , Stefan Wildt
发明人： Tillman Gerngross , Huijuan Li , Stefan Wildt
IPC分类号： C07K16/28 , C07H21/04 , C12P21/06 , A61K39/395 , C12N5/06
CPC分类号： C07K16/00 , A01K2217/075 , C07K16/2896 , C07K2317/24 , C07K2317/41 , C12N9/1051 , C12P21/005
摘要： The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is Man3GlcNAc2.
摘要翻译： 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及包含具有特定富含N-聚糖结构的抗体的药物组合物，其中所述N-聚糖结构是Man 3 GlcNAc 2。
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32.发明申请Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform 审中-公开主要包含GlcNAc2Man3GlcNAc2糖型的免疫球蛋白公开(公告)号：US20060029604A1
公开(公告)日：2006-02-09
申请号：US11187229
申请日：2005-07-21
申请人： Tillman Gerngross , Huijuan Li , Stefan Wildt
发明人： Tillman Gerngross , Huijuan Li , Stefan Wildt
IPC分类号： A61K39/395 , C07H21/04 , C12P21/06 , C12N5/06 , C07K16/28
CPC分类号： C07K16/00 , C07K16/2896 , C07K2317/41 , C07K2317/52 , C07K2317/732
摘要： The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is GlcNAc2Man3GlcNAc2.
摘要翻译： 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及药物组合物，其包含具有特定富集的N-聚糖结构的抗体，其中所述N-聚糖结构是GlcNAc 2 N 3 GlcNAc 2 。
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33.发明授权Yeast strain for the production of proteins with modified O-glycosylation 有权用于生产具有修饰的O-糖基化的蛋白质的酵母菌株公开(公告)号：US08936918B2
公开(公告)日：2015-01-20
申请号：US13978714
申请日：2012-02-20
申请人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
CPC分类号： C07K14/39 , C12N9/90 , C12P21/005
摘要： Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
摘要翻译： 已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。 糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。
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公开(公告)号：US09260712B2
公开(公告)日：2016-02-16
申请号：US13251410
申请日：2011-10-03
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
摘要翻译： 描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。 该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。
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35.发明申请公开(公告)号：US20130295608A1
公开(公告)日：2013-11-07
申请号：US13978714
申请日：2012-02-20
申请人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
IPC分类号： C07K14/39
CPC分类号： C07K14/39 , C12N9/90 , C12P21/005
摘要： Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
摘要翻译： 已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。 糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。
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公开(公告)号：US20100009866A1
公开(公告)日：2010-01-14
申请号：US12489900
申请日：2009-06-23
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
摘要翻译： 描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。 该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。
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37.发明授权Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins 有权在糖蛋白生产中消除聚糖的甘露糖基磷酸化的方法公开(公告)号：US07259007B2
公开(公告)日：2007-08-21
申请号：US11020808
申请日：2004-12-22
CPC分类号： C12N9/1051 , C07K14/39 , C12P21/005
摘要： The present invention relates to the elimination of mannosylphosphorylation on the glycans of glycoproteins in the yeast genus Pichia. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolated P. pastoris MNN4B gene. The present invention further relates to methods for producing modified glycan structures in host cells that are free of glycan mannosylphosphorylation.
摘要翻译： 本发明涉及消除酵母属毕赤酵母中糖蛋白聚糖上的甘露糖基磷酸化。 消除甘露糖基磷酸化糖蛋白是由PNO1基因和新分离的巴斯德毕赤酵母MNN4B基因的破坏产生的。 本发明还涉及在不含聚糖甘露糖基磷酸化的宿主细胞中产生修饰的聚糖结构的方法。
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38.发明授权Method for producing proteins in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens 有权在巴斯德毕赤酵母中生产蛋白质的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交叉结合活性公开(公告)号：US09139632B2
公开(公告)日：2015-09-22
申请号：US13501350
申请日：2010-10-11
申请人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
IPC分类号： C12P21/06 , C12N1/00 , C12N5/00 , C12P21/04 , C07K1/00 , C07K14/505 , C07K14/47 , C07K14/485 , C07K14/52 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/715 , C07K14/755 , C07K14/81 , C07K16/18 , C07K16/22 , C12N9/10 , C12P21/00
CPC分类号： C07K14/505 , C07K14/47 , C07K14/4715 , C07K14/4721 , C07K14/4743 , C07K14/485 , C07K14/52 , C07K14/523 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/70546 , C07K14/70578 , C07K14/7151 , C07K14/755 , C07K14/8114 , C07K14/8125 , C07K14/8128 , C07K16/18 , C07K16/22 , C07K2317/33 , C12N9/1051 , C12P21/005
摘要： Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要翻译： 描述了在针对宿主细胞抗原的抗体中缺乏可检测的交联结合活性的巴斯德毕赤酵母中产生蛋白质和糖蛋白的方法。 具体地说，描述了相对于N-聚糖或O-聚糖不显示“ - - 甘露糖基转移酶2”活性的重组毕赤酵母菌株并且不显示至少一种选自β-甘露糖基转移酶1， 3和4活性产生重组蛋白和糖蛋白。 这些重组毕赤酵母菌株可以产生在其上缺乏可检测的α-甘露糖苷酶抗性和甘露糖残基的蛋白质和糖蛋白，因此对抗宿主细胞抗原的抗体缺乏交叉结合活性。 进一步描述了在巴斯德毕赤酵母中生产双唾液酸化人促红细胞生成素的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交联结合活性。
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39.发明申请METHOD FOR PRODUCING PROTEINS IN PICHIA PASTORIS THAT LACK DETECTABLE CROSS BINDING ACTIVITY TO ANTIBODIES AGAINST HOST CELL ANTIGENS 有权在PICHIA PASTORIS中生产蛋白质的方法，将检测到的交叉结合活性与抗宿主细胞抗原抗体公开(公告)号：US20120232007A1
公开(公告)日：2012-09-13
申请号：US13501350
申请日：2010-10-11
申请人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
CPC分类号： C07K14/505 , C07K14/47 , C07K14/4715 , C07K14/4721 , C07K14/4743 , C07K14/485 , C07K14/52 , C07K14/523 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/70546 , C07K14/70578 , C07K14/7151 , C07K14/755 , C07K14/8114 , C07K14/8125 , C07K14/8128 , C07K16/18 , C07K16/22 , C07K2317/33 , C12N9/1051 , C12P21/005
摘要： Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要翻译： 描述了在针对宿主细胞抗原的抗体中缺乏可检测的交联结合活性的巴斯德毕赤酵母中产生蛋白质和糖蛋白的方法。 具体地说，描述了相对于N-聚糖或O-聚糖不显示“ - - 甘露糖基转移酶2”活性的重组毕赤酵母菌株并且不显示至少一种选自β-甘露糖基转移酶1， 3和4活性产生重组蛋白和糖蛋白。 这些重组毕赤酵母菌株可以产生在其上缺乏可检测的α-甘露糖苷酶抗性和甘露糖残基的蛋白质和糖蛋白，因此对抗宿主细胞抗原的抗体缺乏交叉结合活性。 进一步描述了在巴斯德毕赤酵母中生产双唾液酸化人促红细胞生成素的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交联结合活性。
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公开(公告)号：US20120021948A1
公开(公告)日：2012-01-26
申请号：US13251410
申请日：2011-10-03
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
摘要翻译： 描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。 该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。
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