公开(公告)号：US20230251277A1

公开(公告)日：2023-08-10

申请号：US18125708

申请日：2023-03-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Silvia Baecher ,  Andrea Geistanger ,  Dominik Gruber ,  Hans-Peter Josel

IPC: G01N33/82

CPC classification number: G01N33/82

Abstract: The present invention relates to a method and the use thereof for for determining the level of Vitamin D and metabolites thereof. Further, it is an object of the present invention to provide a kit and the use thereof for determining the level of Vitamin D and metabolites thereof.

公开(公告)号：US11125695B2

公开(公告)日：2021-09-21

申请号：US16111646

申请日：2018-08-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Herbert Andres ,  Michaela Windfuhr ,  Oliver Larbolette ,  Stefan Quint

IPC: G01N21/76 ,  G01N21/66 ,  G01N33/543 ,  C07F15/00 ,  G01N27/30

Abstract: The disclosure concerns methods for the detection of an analyte in a sample by electrochemiluminescence using new reagent compositions. New reagent compositions, reagent kits for measuring electrochemiluminscence (ECL) and electrochemiluminescence detection methods using the new reagent compositions are disclosed. In particular, the disclosure relates to the use of novel combinations of compounds which can be used in said measurements to provide improved assay performance.

公开(公告)号：US20210140983A1

公开(公告)日：2021-05-13

申请号：US17155054

申请日：2021-01-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Nicole Pirkl

IPC: G01N33/74 ,  C07D401/06 ,  G01N33/58

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

公开(公告)号：US20200003666A1

公开(公告)日：2020-01-02

申请号：US16569700

申请日：2019-09-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Eloisa Lopez-Calle ,  Ewelina Hegel ,  Thomas Fischer ,  Caroline Bylda ,  Hans-Peter Josel ,  Josef Roedl

IPC: G01N1/40 ,  G01N33/74 ,  G01N33/82 ,  G01N33/94

Abstract: The present disclosure relates to an in vitro method for releasing analytes from a sample involving contacting the sample with (i) a chaotropic agent, (ii) an organic solvent, (iii) a detergent, and (iv) at least one agent providing bicarbonate ions. The present disclosure further relates to a release agent for releasing analytes from a sample having the aforesaid compounds. Moreover, the present disclosure relates to uses, kits, methods and devices related to the method and the release agent of the present disclosure.

公开(公告)号：US20190324025A1

公开(公告)日：2019-10-24

申请号：US16450181

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Lars Hillringhaus ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Florian Huber ,  Hans-Peter Josel ,  Thomas Meier ,  Michael Schraeml ,  Edgar Voss

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20170269068A1

公开(公告)日：2017-09-21

申请号：US15614856

申请日：2017-06-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Michael Gerg ,  Hans-Peter Josel ,  Christian Vogl

IPC: G01N33/537 ,  G01N33/82 ,  C07K16/26 ,  G01N33/541

CPC classification number: G01N33/537 ,  C07K16/26 ,  G01N33/541 ,  G01N33/82

Abstract: The present invention concerns an in vitro method for measurement of 25-hydroxyvitamin D, wherein the potentially interfering compound 24,25-dihydroxyvitamin D3 is blocked by a binding agent specifically binding to 24,25-dihydroxyvitamin D3 and not binding to 25-hydroxyvitamin D.

公开(公告)号：US20150148536A1

公开(公告)日：2015-05-28

申请号：US14610110

申请日：2015-01-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Cysewski ,  Luisa de Cola ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Elena Longhi ,  Gaston Hubertus Maria Vondenhoff

IPC: C07F15/00 ,  H01L51/00 ,  G01N33/58

CPC classification number: C07F15/0033 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1029 ,  C09K2211/185 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/30 ,  H01L51/0085 ,  H01L51/009 ,  H01L51/5012

Abstract: The present invention relates to novel bis-iridium-based Ir(III) complexes, labels manufactured using these complexes and a method for producing such complexes.

Abstract translation: 本发明涉及新的基于二铱的Ir（III）络合物，使用这些络合物制备的标记物和这种络合物的制备方法。

公开(公告)号：US08658109B2

公开(公告)日：2014-02-25

申请号：US13766297

申请日：2013-02-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Michael Gerg ,  Rupert Hermann ,  Rosa Isabel Saez Diaz

IPC: B01L3/00 ,  C12Q1/68 ,  C12P19/34

CPC classification number: G01N1/30

Abstract: The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Abstract translation: 本公开涉及新颖的双马来酸酐，以及令人惊奇的发现，即双马来酸酐交联剂可用于保存/固定细胞或组织样品。 各种双马来酸酐交联剂扫描用于需要固定细胞或组织样品的方法。 这些试剂和方法在需要除去固定剂以便于与其它试剂分析的方法中特别有用。 本发明的试剂和方法使得可以使用诸如免疫组织化学，荧光原位杂交，RT-PCR等的分析方法更容易地可靠地测定各种蛋白质，核酸等。

公开(公告)号：US20240400672A1

公开(公告)日：2024-12-05

申请号：US18662694

申请日：2024-05-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Hans-Peter Josel ,  Ute Jucknischke ,  Holger Lehmann ,  Anastasia Markina ,  Giuseppe Prencipe ,  Leopold von Proff ,  Michael Schraeml ,  Steinar Halldórsson

IPC: C07K16/26 ,  G01N33/78

Abstract: The present invention provides a novel monoclonal antibody specifically binding to L-Thyroxine (T4) and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such monoclonal antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such monoclonal antibodies. The monoclonal antibody of the invention comprises a heavy chain variable domain (VH) comprising V or A in position 33; Y in position 50; W in position 52; I in position 98, G, A or V in position 99; Y in position 100; and I in position 100b; and a light chain variable domain (VL) comprising amino acids H or Y in position 28; N or K in position 29; W in position 32; G or A in position 91; Y, W or F in position 92; S or T in position 93; Y or F in position 95b; N, S, T or Q in position 95c; and H in position 96, wherein the positions of the amino acids in the VH and the VL are indicated according to the Kabat numbering scheme, respectively.

公开(公告)号：US20230127289A1

公开(公告)日：2023-04-27

申请号：US17990714

申请日：2022-11-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Hahn ,  Dieter Heindl ,  Thomas Hoffmann ,  Hans-Peter Josel ,  Uwe Kobold ,  Hannes Kuchelmeister ,  Simon Ferdinand Loibl ,  Daniela Mazzier ,  Jelena Milic ,  Giuseppe Prencipe ,  Martin Rempt ,  Christoph Seidel

IPC: C07D213/56 ,  C07D401/04 ,  C07D213/74 ,  C07D213/30 ,  H01J49/00

Abstract: The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.