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    Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
    31.
    发明申请
    Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells 审中-公开
    为平滑肌细胞和/或内皮细胞提供组织嗜性的基因递送载体

    公开(公告)号：US20050169891A1

    公开(公告)日：2005-08-04

    申请号：US11018669

    申请日：2004-12-20

    申请人： Ronald Vogels ,  Menzo Havenga ,  Abraham Bout

    发明人： Ronald Vogels ,  Menzo Havenga ,  Abraham Bout

    IPC分类号： A61K38/00 ,  A61K48/00 ,  C07K14/075 ,  C12N15/861

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/6018

    摘要： A gene delivery vehicle having been provided with at least a tissue tropism for cells selected from the group of smooth muscle cells, endothelial cells, and/or liver cells. The tissue tropism is generally provided by a virus capsid, such as one comprising protein fragments from at least two different viruses, such as two different adenoviruses, including adenovirus of subgroup C or subgroup B (for example, adenovirus 16). The protein fragments can comprise a tissue tropism-determining fragment of a fiber protein derived from a subgroup B adenovirus. Also, cells for producing such gene delivery vehicles and pharmaceutical compositions containing these gene delivery vehicles are provided. Further, a method is disclosed for delivering nucleic acid to cells such as smooth muscle cells and/or endothelial cells which involves administering to the cells an adenovirus capsid having proteins from at least two different adenoviruses and wherein at least a tissue tropism-determining fragment of a fiber protein is derived from a subgroup B adenovirus. Particular constructs are also disclosed.

    摘要翻译： 具有至少一种选自平滑肌细胞，内皮细胞和/或肝细胞的细胞的组织嗜性的基因递送载体。 组织向性通常由病毒衣壳提供，例如包含来自至少两种不同病毒的蛋白质片段的病毒衣壳，例如两种不同的腺病毒，包括亚组C或亚组B的腺病毒（例如，腺病毒16）。 蛋白质片段可以包含衍生自亚组B腺病毒的纤维蛋白质的组织向性决定片段。 此外，提供了用于产生这种基因递送载体的细胞和含有这些基因递送载体的药物组合物。 此外，公开了一种用于将核酸递送至诸如平滑肌细胞和/或内皮细胞的细胞的方法，其涉及向细胞施用具有来自至少两种不同腺病毒的蛋白质的腺病毒衣壳，并且其中至少一种组织向性确定片段 纤维蛋白源自B亚型腺病毒亚组。 还公开了特定的构建体。

    Packaging systems for human recombinant adenovirus to be used in gene therapy
    32.
    发明授权
    Packaging systems for human recombinant adenovirus to be used in gene therapy 失效
    用于基因治疗的人重组腺病毒的包装系统

    公开(公告)号：US06670188B1

    公开(公告)日：2003-12-30

    申请号：US09065752

    申请日：1998-04-24

    申请人： Ronald Vogels ,  Abraham Bout

    发明人： Ronald Vogels ,  Abraham Bout

    IPC分类号： C07H2104

    CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/60

    摘要： The invention provides improved methods and products based on adenoviral materials which can advantageously be used in for instance gene therapy. In one aspect an adenoviral vector is provided which has no overlap with a suitable packaging cell line which is another aspect of the invention. This combination excludes the possibility of homologous recombination, thereby excluding the possibility of the formation of replication competent adenovirus. In another aspect an adenovirus based helper construct which by its size is incapable of being encapsidated. This helper virus can be transferred into any suitable host cell making it a packaging cell. Further, a number of useful mutations to adenoviral based materials and combinations of such mutations are disclosed, which all have in common the safety of the methods and the products, in particular avoiding the production of replication competent adenovirus and/or interference with the immune system. Further, a method of intracellular amplification is provided.

    摘要翻译： 本发明提供了可以有利地用于例如基因治疗的基于腺病毒材料的改进的方法和产品。 在一个方面，提供了与本发明另一方面的合适的包装细胞系重叠的腺病毒载体。 该组合不包括同源重组的可能性，从而排除形成复制型腺病毒的可能性。 在另一方面，基于腺病毒的辅助构建体，其大小不能被包被。 这种辅助病毒可以转移到任何合适的宿主细胞中，使其成为包装细胞。 此外，公开了一些对基于腺病毒的材料和这种突变的组合的有用的突变，其都具有方法和产品的安全性，特别是避免产生可复制的腺病毒和/或免疫系统的干扰 。 此外，提供了细胞内扩增的方法。

    High throughput screening of gene function using adenoviral libraries for functional genomics applications
    33.
    发明授权
    High throughput screening of gene function using adenoviral libraries for functional genomics applications 失效
    基因功能的高通量筛选使用腺病毒文库进行功能基因组学应用

    公开(公告)号：US06413776B1

    公开(公告)日：2002-07-02

    申请号：US09097239

    申请日：1998-06-12

    申请人： Ronald Vogels ,  Abraham Bout ,  Helmuth H. G. van Es ,  Govert Shouten

    发明人： Ronald Vogels ,  Abraham Bout ,  Helmuth H. G. van Es ,  Govert Shouten

    IPC分类号： C12N510

    CPC分类号： C12N15/1034 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2830/42

    摘要： Novel adenovirus vectors and methods for their use are provided to determine the function of the product(s) of one or more sample nucleic acids. The sample nucleic acids are synthetic oligonucleotides, DNA, or cDNA and encode polypeptides, antisense nucleic acids or GSEs. The sample nucleic acids are expressed in a host by recombinant adenovirus vectors to alter at least one phenotype of the host. The altered phenotype(s) is identified as a means to assign a biological function to the product(s) encoded by the sample nucleic acid(s).

    摘要翻译： 提供新型腺病毒载体及其使用方法以确定一种或多种样品核酸的产物的功能。 样品核酸是合成寡核苷酸，DNA或cDNA，并编码多肽，反义核酸或GSE。 样品核酸通过重组腺病毒载体在宿主中表达以改变宿主的至少一种表型。 改变的表型被鉴定为将样品核酸编码的产物赋予生物学功能的手段。

    Multivalent vaccines comprising recombinant viral vectors
    35.
    发明申请
    Multivalent vaccines comprising recombinant viral vectors 有权
    包含重组病毒载体的多价疫苗

    公开(公告)号：US20110281347A1

    公开(公告)日：2011-11-17

    申请号：US13134190

    申请日：2011-05-31

    申请人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Radosevic

    发明人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Radosevic

    IPC分类号： C12N15/63

    CPC分类号： A61K39/04 ,  A61K35/761 ,  A61K2039/523 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/55544 ,  C07K14/35 ,  C07K2319/00 ,  C12N2710/10343

    摘要： Described are vaccines comprising recombinant vectors, such as recombinant adenoviruses. The vectors comprise heterologous nucleic acids encoding at least two antigens from one or more tuberculosis-causing bacilli. Also described is the use of specific protease recognition sites linking antigens through which the encoded antigens are separated upon cleavage. After cleavage, the antigens contribute to the immune response in a separate manner. The recombinant vectors may comprise a nucleic acid encoding the protease cleaving the linkers and separating the antigens. Also described is the use of genetic adjuvants encoded by the recombinant vectors, wherein such genetic adjuvants may also be cleaved through the presence of the cleavable linkers and the specific protease.

    摘要翻译： 描述了包含重组载体例如重组腺病毒的疫苗。 载体包含编码来自一种或多种结核分枝杆菌的至少两种抗原的异源核酸。 还描述了连接抗原的特异性蛋白酶识别位点的使用，通过该抗原，编码的抗原在切割时被分离。 在切割后，抗原以单独的方式有助于免疫应答。 重组载体可以包含编码切割接头并分离抗原的蛋白酶的核酸。 还描述了由重组载体编码的遗传佐剂的用途，其中这种遗传佐剂也可以通过可切割接头和特异性蛋白酶的存在来切割。

    MEMS resonator
    40.
    发明授权
    MEMS resonator 有权
    MEMS谐振器

    公开(公告)号：US08729973B2

    公开(公告)日：2014-05-20

    申请号：US13062877

    申请日：2009-09-07

    申请人： Jozef Thomas Martinus van Beek ,  Ronald Vogels

    发明人： Jozef Thomas Martinus van Beek ,  Ronald Vogels

    IPC分类号： H03B5/30 ,  H03B1/02 ,  H03L1/04 ,  B81B7/02

    CPC分类号： H03H9/02448

    摘要： A MEMS resonator comprises a resonator body (34), and an anchor (32) which provides a fixed connection between the resonator body (34) and a support body. A resistive heating element (R1,R2) and a feedback control system are used to maintain the resonator body (34) at a constant temperature. A location for thermally coupling the anchor (32) to the resistive heating element (R1,R2) is selected which has a lowest dependency of its temperature on the ambient temperature during the operation of the feedback control.

    摘要翻译： MEMS谐振器包括谐振器主体（34）和在谐振器主体（34）和支撑体之间提供固定连接的锚固件（32）。 使用电阻加热元件（R1，R2）和反馈控制系统将谐振器本体（34）保持在恒定温度。 选择用于将锚固件（32）热耦合到电阻加热元件（R1，R2）的位置，其在反馈控制的操作期间具有最低的温度对环境温度的依赖性。