公开(公告)号：US20080254532A1

公开(公告)日：2008-10-16

申请号：US11981155

申请日：2007-10-30

Applicant: Ronald Chang ,  Lee A. Christel ,  Gregory T.A. Kovacs ,  William A. McMillan ,  M. Allen Northrup ,  Kurt E. Petersen ,  Farzad Pourahmadi ,  Steven J. Young ,  Robert Yuan ,  Douglas B. Dority

Inventor： Ronald Chang ,  Lee A. Christel ,  Gregory T.A. Kovacs ,  William A. McMillan ,  M. Allen Northrup ,  Kurt E. Petersen ,  Farzad Pourahmadi ,  Steven J. Young ,  Robert Yuan ,  Douglas B. Dority

IPC: C12M1/02

CPC classification number: G01N21/75 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/0081 ,  B01J2219/00822 ,  B01J2219/00828 ,  B01J2219/00833 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01J2219/00986 ,  B01L3/505 ,  B01L3/508 ,  B01L7/52 ,  B01L2300/042 ,  B01L2300/0809 ,  B01L2300/0858 ,  B01L2300/168 ,  B01L2300/1805 ,  F28D2021/0077 ,  F28F3/12 ,  F28F21/04 ,  F28F2280/10 ,  G01N21/0303 ,  G01N21/0332 ,  G01N2035/00326

Abstract: A reaction vessel having a reaction chamber for holding a sample is fabricated by producing a housing having a rigid frame defining the minor walls of the chamber. The housing also defines a port for introducing fluid into the chamber. At least one sheet or film is attached to the rigid frame to form at least one major wall of the chamber. In preferred embodiments, two sheets or films are attached to opposite sides of the rigid frame to form two opposing major walls of the chamber, the major walls being connected to each other by the minor walls.

Abstract translation: 具有用于保持样品的反应室的反应容器通过制造具有限定室的小壁的刚性框架的壳体来制造。 壳体还限定了用于将流体引入腔室的端口。 至少一个片或薄膜连接到刚性框架以形成腔室的至少一个主壁。 在优选实施例中，两个片或膜连接到刚性框架的相对侧面以形成腔室的两个相对的主壁，主壁通过次壁彼此连接。

公开(公告)号：US07188001B2

公开(公告)日：2007-03-06

申请号：US10353186

申请日：2003-01-27

Applicant: Steven J. Young ,  Gregory T. A. Kovacs ,  M. Allen Northrup ,  Kurt E. Petersen ,  William A. McMillan ,  Konstantin Othmer ,  Lee A. Christel

Inventor： Steven J. Young ,  Gregory T. A. Kovacs ,  M. Allen Northrup ,  Kurt E. Petersen ,  William A. McMillan ,  Konstantin Othmer ,  Lee A. Christel

IPC: G05B21/00 ,  G01N25/20 ,  G01N21/00

CPC classification number: B01L7/52 ,  G05B13/021 ,  G05D23/1913 ,  Y10T436/12

Abstract: A system for controlling the temperature of a reaction mixture comprises at least one heating device for heating the mixture and a power regulator for regulating the amount of power supplied to the heating device. A controller in communication with the power regulator includes program instructions for heating the reaction mixture by setting a variable target temperature that initially exceeds a desired setpoint temperature for the mixture. When the heating device reaches a threshold temperature, the variable target temperature is decreased to the desired setpoint temperature. In another embodiment, the controller includes an adaptive control program for dynamically adjusting the duration or intensity of power pulses provided to the heating device.

Abstract translation: 用于控制反应混合物的温度的系统包括至少一个用于加热混合物的加热装置和用于调节供应给加热装置的功率量的功率调节器。 与功率调节器通信的控制器包括用于通过设定最初超过混合物的期望设定点温度的可变目标温度来加热反应混合物的程序指令。 当加热装置达到阈值温度时，可变目标温度降低到所需的设定点温度。 在另一个实施例中，控制器包括用于动态调整提供给加热装置的功率脉冲的持续时间或强度的自适应控制程序。

公开(公告)号：US06942971B2

公开(公告)日：2005-09-13

申请号：US10027404

申请日：2001-12-19

Applicant: William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

Inventor： William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

IPC: B01L7/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N9/98 ,  C12P19/34 ,  C12Q1/00 ,  C12Q1/68 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N35/00 ,  G06F19/00

CPC classification number: C12Q1/6851 ,  B01L7/52 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/0097 ,  G01N2201/062 ,  G01N2201/0627 ,  C12Q2537/165 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.

Abstract translation: 用于确定核酸扩增反应中的阈值（例如，阈值循环数或时间值）的装置包括检测机制，用于在扩增反应期间的多个不同时间测量至少一个强度为 与在反应中被扩增的核酸序列的量有关。 与检测机制通信的控制器被编程为存储定义核酸序列的生长曲线的信号值，确定生长曲线的导数，并计算与该衍生物的特征相关联的周期数或时间值。

公开(公告)号：US06911327B2

公开(公告)日：2005-06-28

申请号：US09808706

申请日：2001-03-14

Applicant: William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

Inventor： William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

IPC: B01L7/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N9/98 ,  C12P19/34 ,  C12Q1/00 ,  C12Q1/68 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N35/00 ,  G06F19/00

CPC classification number: C12Q1/6851 ,  B01L7/52 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/0097 ,  G01N2201/062 ,  G01N2201/0627 ,  C12Q2537/165 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample comprises amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample and known starting quantities of a calibration nucleic acid sequence in respective calibration samples; and determining a respective threshold value for each of the nucleic acid sequences using a derivative of a growth curve derived for the sequence. The starting quantity of the target nucleic acid sequence in the test sample is determined using the threshold value determined for the target sequence and a calibration curve derived from the threshold values determined for the known starting quantities of the calibration nucleic acid sequences.

Abstract translation: 用于测定测试样品中目标核酸序列的未知起始量的方法包括扩增测试样品中目标核酸序列的未知起始量和已知的各校准样品中校准核酸序列的起始量; 以及使用由该序列导出的生长曲线的衍生物来确定每个核酸序列的相应阈值。 使用针对靶序列确定的阈值和从针对校准核酸序列的已知起始量确定的阈值得到的校准曲线来确定测试样品中靶核酸序列的起始量。

公开(公告)号：US06783934B1

公开(公告)日：2004-08-31

申请号：US09562195

申请日：2000-05-01

Applicant: William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

Inventor： William A. McMillan ,  Lee A. Christel ,  David A. Borkholder ,  Steven J. Young

IPC: C12Q168

CPC classification number: C12Q1/6851 ,  B01L7/52 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/0097 ,  G01N2201/062 ,  G01N2201/0627 ,  C12Q2537/165 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: A method for determining a threshold cycle number or time value in a nucleic acid amplification reaction comprising the steps of amplifying a nucleic acid sequence in a reaction mixture; at a plurality of different times during the amplification reaction, measuring at least one signal whose intensity is related to the quantity of the nucleic acid sequence in the reaction mixture; and storing signal values defining a growth curve for the nucleic acid sequence. A derivative of the growth curve is determined and the threshold cycle number or time value is calculated as a cycle number or time value corresponding to a characteristic (e.g., maximum, minimum, or zero-crossing) of the derivative.

Abstract translation: 一种用于确定核酸扩增反应中阈值循环数或时间值的方法，包括以下步骤：扩增反应混合物中的核酸序列; 在扩增反应期间的多个不同时间，测量强度与反应混合物中的核酸序列的量相关的至少一个信号; 以及存储限定所述核酸序列的生长曲线的信号值。 确定生长曲线的导数，并且将阈值循环数或时间值计算为对应于该导数的特征（例如，最大值，最小值或过零点）的周期数或时间值。

公开(公告)号：US5645949A

公开(公告)日：1997-07-08

申请号：US482950

申请日：1995-06-07

Applicant: Steven J. Young

Inventor： Steven J. Young

IPC: G01R31/00 ,  G01R31/36 ,  H01M6/42 ,  H01M10/42 ,  H01M10/48 ,  H02J7/00 ,  H01M14/00

CPC classification number: G01R31/3648 ,  G01R31/3655 ,  H01M10/4257 ,  H01M10/48 ,  H01M10/486 ,  H02J7/0011 ,  H02J7/0018 ,  G01R31/006 ,  H01M6/42

Abstract: A battery cell having a positive terminal, a negative terminal and a power producing core section (e.g., electrolyte) for systems, such as computer systems, cellular phones, etc. The battery cell also includes an internal circuit to monitor the state of the battery cell. The state that is monitored may include the temperature, charge level of the battery core section, the discharge/charge rate. The circuit may control the battery cell (e.g., cause charging of the battery cell). This internal circuit may be an integrated circuit, such as a microprocessor.

公开(公告)号：US5622789A

公开(公告)日：1997-04-22

申请号：US310802

申请日：1994-09-12

Applicant: Steven J. Young

Inventor： Steven J. Young

IPC: G01R31/00 ,  G01R31/36 ,  H01M6/42 ,  H01M10/42 ,  H01M10/48 ,  H02J7/00 ,  H01M2/20 ,  H01M2/22 ,  H01M2/30

CPC classification number: G01R31/3648 ,  G01R31/3655 ,  H01M10/4257 ,  H01M10/48 ,  H01M10/486 ,  H02J7/0011 ,  H02J7/0018 ,  G01R31/006 ,  H01M6/42

Abstract: A battery cell having a positive terminal, a negative terminal and a power producing core section (e.g., electrolyte) for systems, such as computer systems, cellular phones, etc. The battery cell also includes an internal circuit to monitor the state of the battery cell. The state that is monitored may include the temperature, charge level of the battery core section, the discharge/charge rate. The circuit may control the battery cell (e.g., cause charging of the battery cell). This internal circuit may be an integrated circuit, such as a microprocessor.

Abstract translation: 具有用于诸如计算机系统，蜂窝电话等的系统的正极端子，负极端子和功率产生芯部分（例如，电解质）的电池单体。电池单元还包括用于监视电池状态的内部电路 细胞。 被监测的状态可以包括电池芯部分的温度，充电水平，放电/充电速率。 电路可以控制电池单元（例如，引起电池单元的充电）。 该内部电路可以是诸如微处理器的集成电路。