中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

94 records (took 0.024 seconds)

    Inkjet gene printing
    38.
    发明授权
    Inkjet gene printing 有权

    公开(公告)号：US09598704B2

    公开(公告)日：2017-03-21

    申请号：US14697704

    申请日：2015-04-28

    Applicant: Wake Forest University Health Sciences

    Inventor： Tao Xu ,  James J. Yoo ,  Anthony Atala

    IPC: C12N15/87 ,  C12N5/00 ,  C12N15/63 ,  C12N15/89 ,  A61K31/713

    CPC classification number: C12N15/895 ,  A61K31/713 ,  C12N15/63 ,  C12N15/87 ,  C12N15/89

    Abstract: Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

    ENCAPSULATED CELLS FOR HORMONE REPLACEMENT THERAPY
    39.
    发明申请
    ENCAPSULATED CELLS FOR HORMONE REPLACEMENT THERAPY 审中-公开
    用于激素替代治疗的包封细胞

    公开(公告)号：US20160206665A1

    公开(公告)日：2016-07-21

    申请号：US15014536

    申请日：2016-02-03

    Applicant: Wake Forest University Health Sciences

    Inventor： Emmanuel Opara ,  James J. Yoo ,  Justin M. Saul ,  Sittadjody Sivanandane ,  Anthony Atala

    IPC: A61K35/54 ,  A61K9/50

    CPC classification number: A61K35/54 ,  A61K9/167 ,  A61K9/5036 ,  A61K9/5073 ,  A61K31/565 ,  A61K31/57 ,  A61K2300/00

    Abstract: A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.

    Abstract translation: 描述了包含微胶囊的组合物，包含活哺乳动物卵巢颗粒细胞和活哺乳动物卵巢细胞的微胶囊。 在一些实施方案中，颗粒细胞和细胞被包含在组合物中的分离的微胶囊中; 在一些实施方案中，颗粒细胞和细胞在组合物中一起包含在相同的微胶囊中。组合物可用于雌激素，并且任选地也可用于孕酮，递送，因此优选是游离的或基本上不含卵母细胞。 还描述了使用它们的方法和含有它们的药物制剂。

    INTEGRATED ORGAN AND TISSUE PRINTING METHODS, SYSTEM AND APPARATUS
    40.
    发明申请
    INTEGRATED ORGAN AND TISSUE PRINTING METHODS, SYSTEM AND APPARATUS 审中-公开
    一体化组织和组织印刷方法，系统和装置

    公开(公告)号：US20150119994A1

    公开(公告)日：2015-04-30

    申请号：US14476359

    申请日：2014-09-03

    Applicant: Wake Forest University Health Sciences

    Inventor： Hyun-Wook Kang ,  Sang Jin Lee ,  Anthony Atala ,  James J. Yoo

    IPC: B29C67/00 ,  A61L27/52 ,  A61L27/26 ,  A61L27/38

    CPC classification number: B29C64/112 ,  A61K35/12 ,  A61L27/20 ,  A61L27/222 ,  A61L27/225 ,  A61L27/26 ,  A61L27/38 ,  A61L27/52 ,  B29C64/124 ,  B29C64/386 ,  B29C64/393 ,  B29K2105/0005 ,  B29K2267/04 ,  B29L2031/7532 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/50 ,  C12M33/00 ,  C08L5/08

    Abstract: A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

    Abstract translation: 一种制造器官或组织的方法包括：（a）提供含有结构支持聚合物的第一分配器和包含含活细胞组合物的第二分配器; （b）从所述第一和第二分配器在所述支撑件上沉积一层，所述层包括结构支撑聚合物和所述含细胞的组合物; 然后（c）重复地重复所述沉积步骤多次以形成多个层，其中每个所述层中的单独和离散区域包括所述支撑聚合物或所述含细胞组合物中的一种或另一种， 从而产生提供包含结构支持区域和含细胞区域的复合三维结构。 还描述了用于实施由该方法生产的方法和复合产品的装置。

Patent Agency Ranking